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Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus

SEM of cells on substrates.Representative SEM micrographs of cells incubated on substrates for 3 days, a) C-HA and b) F-HA at low magnification (1000x); c) C-HA and b) F-HA at higher magnification (3000x). Arrows pointing on some of the cells.
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pone.0120381.g005: SEM of cells on substrates.Representative SEM micrographs of cells incubated on substrates for 3 days, a) C-HA and b) F-HA at low magnification (1000x); c) C-HA and b) F-HA at higher magnification (3000x). Arrows pointing on some of the cells.

Mentions: Macrophage morphology on the HA discs was assessed at day 3 using SEM. Low magnification micrographs clearly show C-HA surface being covered by a large number of cells (Fig. 5A), whereas only a few cells adhered to the F-HA (Fig. 5B). Moreover, a difference in morphology could also be seen at higher magnifications. Cells on C-HA substrates were more spread out compared to the rounder morphology of macrophages cultured on F-HA (Fig. 5D).


Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

SEM of cells on substrates.Representative SEM micrographs of cells incubated on substrates for 3 days, a) C-HA and b) F-HA at low magnification (1000x); c) C-HA and b) F-HA at higher magnification (3000x). Arrows pointing on some of the cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383585&req=5

pone.0120381.g005: SEM of cells on substrates.Representative SEM micrographs of cells incubated on substrates for 3 days, a) C-HA and b) F-HA at low magnification (1000x); c) C-HA and b) F-HA at higher magnification (3000x). Arrows pointing on some of the cells.
Mentions: Macrophage morphology on the HA discs was assessed at day 3 using SEM. Low magnification micrographs clearly show C-HA surface being covered by a large number of cells (Fig. 5A), whereas only a few cells adhered to the F-HA (Fig. 5B). Moreover, a difference in morphology could also be seen at higher magnifications. Cells on C-HA substrates were more spread out compared to the rounder morphology of macrophages cultured on F-HA (Fig. 5D).

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus