Limits...
Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis.

Li Z, Jing W, Peng Y, Zhang XQ, Ma X, Huang LK, Yan YH - PLoS ONE (2015)

Bottom Line: The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars.Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought.Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.

ABSTRACT
The objective of this research was to analyse whether ameliorating drought stress through exogenously applied spermine (Spm) was related to carbohydrate metabolism, dehydrins accumulation and the transcription of genes encoding dehydrins in two white clovers (drought-susceptible cv. 'Ladino' and drought-resistant cv. 'Haifa') under controlled drying conditions for 10 days. The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars. Exogenous Spm led to accumulation of more water-soluble carbohydrates (WSC), sucrose, fructose and sorbitol in both cultivars under drought stress, and also significantly elevated glucose content in leaves of drought-resistant cv. 'Haifa', but had no effect on drought-susceptible cv. 'Ladino'. Accordingly, the key enzyme activities of sucrose and sorbitol metabolism changed along with the application of Spm under drought stress. Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought. In addition, the improved accumulation of dehydrins induced by exogenous Spm coincided with three genes expression which was responsible for dehydrins synthesis. But Spm-induced transcript level of dehydrin genes increased earlier in cv. 'Ladino' than that in cv. 'Haifa'. Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis. There are differences between drought-susceptible and -resistant white clover cultivars related to Spm regulation of WSC metabolism and dehydrins expression.

No MeSH data available.


Related in: MedlinePlus

The effect of exogenous spermine (Spm) on dehydrins synthesis in white clover under drought stress.The effect of exogenous spermine (Spm) on (A) dehydrins expression in leaves of cv. ‘Ladino’, (B) dehydrins expression in leaves of cv. ‘Haifa’, (C) integrated intensity of 33 kDa dehydrin, (D) integrated intensity of 23 kDa dehydrin, (E) integrated intensity of 22 kDa dehydrin under drought stress. L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4383584&req=5

pone.0120708.g008: The effect of exogenous spermine (Spm) on dehydrins synthesis in white clover under drought stress.The effect of exogenous spermine (Spm) on (A) dehydrins expression in leaves of cv. ‘Ladino’, (B) dehydrins expression in leaves of cv. ‘Haifa’, (C) integrated intensity of 33 kDa dehydrin, (D) integrated intensity of 23 kDa dehydrin, (E) integrated intensity of 22 kDa dehydrin under drought stress. L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.

Mentions: The drought-stress inducibility of dehydrins protein synthesis in whiter clover was analysed by using western blot. Drought stress and exogenous Spm induced accumulation of dehydrins in both cultivars. There were three types of dehydrins (33, 23 and 22 kDa) in cv. ‘Ladino’ when subjected to drought stress, while only two types of dehydrins (33 and 22 kDa) were detected in cv. ‘Haifa’ (Fig. 8A,B). Spm-treated plants (‘L+Spm’ and ‘H+Spm’) exhibited obviously higher integrated intensity of 33 kDa dehydrin than non-treated plants (‘L’ and ‘H’) throughout drought stress (Fig. 8C). A 23 kDa dehydrin was induced by drought stress in cv. ‘Ladino’, but not in cv. ‘Haifa’. However, significant difference in the level of 23 kDa dehydrin was not observed between ‘L’ and ‘L+Spm’ treatment under drought stress (Fig. 8D). The change of integrated intensity of 22 kDa dehydrin in both cultivars showed a similar trend as the 33 kDa dehydrin (Fig. 8E).


Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis.

Li Z, Jing W, Peng Y, Zhang XQ, Ma X, Huang LK, Yan YH - PLoS ONE (2015)

The effect of exogenous spermine (Spm) on dehydrins synthesis in white clover under drought stress.The effect of exogenous spermine (Spm) on (A) dehydrins expression in leaves of cv. ‘Ladino’, (B) dehydrins expression in leaves of cv. ‘Haifa’, (C) integrated intensity of 33 kDa dehydrin, (D) integrated intensity of 23 kDa dehydrin, (E) integrated intensity of 22 kDa dehydrin under drought stress. L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383584&req=5

pone.0120708.g008: The effect of exogenous spermine (Spm) on dehydrins synthesis in white clover under drought stress.The effect of exogenous spermine (Spm) on (A) dehydrins expression in leaves of cv. ‘Ladino’, (B) dehydrins expression in leaves of cv. ‘Haifa’, (C) integrated intensity of 33 kDa dehydrin, (D) integrated intensity of 23 kDa dehydrin, (E) integrated intensity of 22 kDa dehydrin under drought stress. L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
Mentions: The drought-stress inducibility of dehydrins protein synthesis in whiter clover was analysed by using western blot. Drought stress and exogenous Spm induced accumulation of dehydrins in both cultivars. There were three types of dehydrins (33, 23 and 22 kDa) in cv. ‘Ladino’ when subjected to drought stress, while only two types of dehydrins (33 and 22 kDa) were detected in cv. ‘Haifa’ (Fig. 8A,B). Spm-treated plants (‘L+Spm’ and ‘H+Spm’) exhibited obviously higher integrated intensity of 33 kDa dehydrin than non-treated plants (‘L’ and ‘H’) throughout drought stress (Fig. 8C). A 23 kDa dehydrin was induced by drought stress in cv. ‘Ladino’, but not in cv. ‘Haifa’. However, significant difference in the level of 23 kDa dehydrin was not observed between ‘L’ and ‘L+Spm’ treatment under drought stress (Fig. 8D). The change of integrated intensity of 22 kDa dehydrin in both cultivars showed a similar trend as the 33 kDa dehydrin (Fig. 8E).

Bottom Line: The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars.Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought.Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.

ABSTRACT
The objective of this research was to analyse whether ameliorating drought stress through exogenously applied spermine (Spm) was related to carbohydrate metabolism, dehydrins accumulation and the transcription of genes encoding dehydrins in two white clovers (drought-susceptible cv. 'Ladino' and drought-resistant cv. 'Haifa') under controlled drying conditions for 10 days. The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars. Exogenous Spm led to accumulation of more water-soluble carbohydrates (WSC), sucrose, fructose and sorbitol in both cultivars under drought stress, and also significantly elevated glucose content in leaves of drought-resistant cv. 'Haifa', but had no effect on drought-susceptible cv. 'Ladino'. Accordingly, the key enzyme activities of sucrose and sorbitol metabolism changed along with the application of Spm under drought stress. Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought. In addition, the improved accumulation of dehydrins induced by exogenous Spm coincided with three genes expression which was responsible for dehydrins synthesis. But Spm-induced transcript level of dehydrin genes increased earlier in cv. 'Ladino' than that in cv. 'Haifa'. Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis. There are differences between drought-susceptible and -resistant white clover cultivars related to Spm regulation of WSC metabolism and dehydrins expression.

No MeSH data available.


Related in: MedlinePlus