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Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis.

Li Z, Jing W, Peng Y, Zhang XQ, Ma X, Huang LK, Yan YH - PLoS ONE (2015)

Bottom Line: The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars.Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought.Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.

ABSTRACT
The objective of this research was to analyse whether ameliorating drought stress through exogenously applied spermine (Spm) was related to carbohydrate metabolism, dehydrins accumulation and the transcription of genes encoding dehydrins in two white clovers (drought-susceptible cv. 'Ladino' and drought-resistant cv. 'Haifa') under controlled drying conditions for 10 days. The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars. Exogenous Spm led to accumulation of more water-soluble carbohydrates (WSC), sucrose, fructose and sorbitol in both cultivars under drought stress, and also significantly elevated glucose content in leaves of drought-resistant cv. 'Haifa', but had no effect on drought-susceptible cv. 'Ladino'. Accordingly, the key enzyme activities of sucrose and sorbitol metabolism changed along with the application of Spm under drought stress. Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought. In addition, the improved accumulation of dehydrins induced by exogenous Spm coincided with three genes expression which was responsible for dehydrins synthesis. But Spm-induced transcript level of dehydrin genes increased earlier in cv. 'Ladino' than that in cv. 'Haifa'. Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis. There are differences between drought-susceptible and -resistant white clover cultivars related to Spm regulation of WSC metabolism and dehydrins expression.

No MeSH data available.


Related in: MedlinePlus

The effect of exogenous spermine (Spm) on (A) sucrose synthetase (SS) and (B) sucrose phosphate synthase (SPS) activity in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
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pone.0120708.g005: The effect of exogenous spermine (Spm) on (A) sucrose synthetase (SS) and (B) sucrose phosphate synthase (SPS) activity in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.

Mentions: Exogenous Spm-treatment in both cultivars decreased SS activity from 0 to 10 d of drought stress. This decreased percentage was 13%, 23% and 22% in cv. ‘Ladino’ at 0, 5, and 10 d of treatment, respectively, and 13%, 14% and 14% in cv. ‘Haifa’, correspondingly (Fig. 5A). SPS activity was relatively unchanged or slightly increased in non-treated plants (‘H’ or ‘L’) during drought stress, but obviously increased by 69% or 33% in ‘L+Spm’ or ‘H+Spm’ treatment after 10 d of drought stress. Accordingly, significant differences of SPS activities between Spm-treated plants (‘L+Spm’ or ‘H+Spm’) and non-treated plants (‘L’ or ‘H’) were observed at 5 and 10 d of drought stress (Fig. 5B).


Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis.

Li Z, Jing W, Peng Y, Zhang XQ, Ma X, Huang LK, Yan YH - PLoS ONE (2015)

The effect of exogenous spermine (Spm) on (A) sucrose synthetase (SS) and (B) sucrose phosphate synthase (SPS) activity in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383584&req=5

pone.0120708.g005: The effect of exogenous spermine (Spm) on (A) sucrose synthetase (SS) and (B) sucrose phosphate synthase (SPS) activity in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
Mentions: Exogenous Spm-treatment in both cultivars decreased SS activity from 0 to 10 d of drought stress. This decreased percentage was 13%, 23% and 22% in cv. ‘Ladino’ at 0, 5, and 10 d of treatment, respectively, and 13%, 14% and 14% in cv. ‘Haifa’, correspondingly (Fig. 5A). SPS activity was relatively unchanged or slightly increased in non-treated plants (‘H’ or ‘L’) during drought stress, but obviously increased by 69% or 33% in ‘L+Spm’ or ‘H+Spm’ treatment after 10 d of drought stress. Accordingly, significant differences of SPS activities between Spm-treated plants (‘L+Spm’ or ‘H+Spm’) and non-treated plants (‘L’ or ‘H’) were observed at 5 and 10 d of drought stress (Fig. 5B).

Bottom Line: The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars.Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought.Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.

ABSTRACT
The objective of this research was to analyse whether ameliorating drought stress through exogenously applied spermine (Spm) was related to carbohydrate metabolism, dehydrins accumulation and the transcription of genes encoding dehydrins in two white clovers (drought-susceptible cv. 'Ladino' and drought-resistant cv. 'Haifa') under controlled drying conditions for 10 days. The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars. Exogenous Spm led to accumulation of more water-soluble carbohydrates (WSC), sucrose, fructose and sorbitol in both cultivars under drought stress, and also significantly elevated glucose content in leaves of drought-resistant cv. 'Haifa', but had no effect on drought-susceptible cv. 'Ladino'. Accordingly, the key enzyme activities of sucrose and sorbitol metabolism changed along with the application of Spm under drought stress. Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought. In addition, the improved accumulation of dehydrins induced by exogenous Spm coincided with three genes expression which was responsible for dehydrins synthesis. But Spm-induced transcript level of dehydrin genes increased earlier in cv. 'Ladino' than that in cv. 'Haifa'. Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis. There are differences between drought-susceptible and -resistant white clover cultivars related to Spm regulation of WSC metabolism and dehydrins expression.

No MeSH data available.


Related in: MedlinePlus