Limits...
Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis.

Li Z, Jing W, Peng Y, Zhang XQ, Ma X, Huang LK, Yan YH - PLoS ONE (2015)

Bottom Line: The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars.Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought.Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.

ABSTRACT
The objective of this research was to analyse whether ameliorating drought stress through exogenously applied spermine (Spm) was related to carbohydrate metabolism, dehydrins accumulation and the transcription of genes encoding dehydrins in two white clovers (drought-susceptible cv. 'Ladino' and drought-resistant cv. 'Haifa') under controlled drying conditions for 10 days. The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars. Exogenous Spm led to accumulation of more water-soluble carbohydrates (WSC), sucrose, fructose and sorbitol in both cultivars under drought stress, and also significantly elevated glucose content in leaves of drought-resistant cv. 'Haifa', but had no effect on drought-susceptible cv. 'Ladino'. Accordingly, the key enzyme activities of sucrose and sorbitol metabolism changed along with the application of Spm under drought stress. Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought. In addition, the improved accumulation of dehydrins induced by exogenous Spm coincided with three genes expression which was responsible for dehydrins synthesis. But Spm-induced transcript level of dehydrin genes increased earlier in cv. 'Ladino' than that in cv. 'Haifa'. Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis. There are differences between drought-susceptible and -resistant white clover cultivars related to Spm regulation of WSC metabolism and dehydrins expression.

No MeSH data available.


Related in: MedlinePlus

The effect of exogenous spermine (Spm) on (A) water-soluble carbohydrates (WSC), (B) sucrose, (C) fructose, and (D) glucose content in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4383584&req=5

pone.0120708.g004: The effect of exogenous spermine (Spm) on (A) water-soluble carbohydrates (WSC), (B) sucrose, (C) fructose, and (D) glucose content in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.

Mentions: Drought stress strongly affected the accumulation of WSC in white clover leaves and content of WSC in four treatments reached to maximum after 10 d of drought stress. Compared to ‘L’ treatment, ‘L+Spm’ had 17% percent higher WSC content at the end of drought stress. ‘H+Spm’ showed significantly higher WSC content than ‘H’ treatment throughout the whole process of drought stress (Fig. 4A). In addition, sucrose contents of ‘L’ and ‘L+Spm’ treatment displayed a ~ 2-fold and ~ 3-fold increase after 5 d of drought, respectively. When drought stress lasted for 5 and 10 d, ‘L+Spm’ accumulated significantly higher sucrose content relative to ‘L’. With the development of drought stress, little change of sucrose content in cv. ‘Haifa’ was observed, while ‘H+Spm’ exhibited significantly higher sucrose content than ‘H’ at the end of drought stress (Fig. 4B). Plants of cv. ‘Haifa’ had significantly higher fructose content than plants of cv. ‘Ladino’ at the beginning. Although without statistically significant difference in fructose between ‘H+Spm’ and ‘H’ at 5 d of drought stress, ‘H+Spm’ displayed significantly higher fructose level after 10 d of water deficit relative to ‘H’ treatment. Fructose content in ‘L+Spm’ also maintained a significantly higher level under 5 and 10 d of drought stress as compared to that in ‘L’ (Fig. 4C). Exogenous Spm-treatment almost had no effect on glucose accumulation in cv. ‘Ladino’ under drought stress. However, glucose content in ‘H+Spm’ was considerably greater than that in ‘H’ under drought stress, and a peak value was visible in ‘H+Spm’ treatment at 5 d of stress (Fig. 4D).


Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis.

Li Z, Jing W, Peng Y, Zhang XQ, Ma X, Huang LK, Yan YH - PLoS ONE (2015)

The effect of exogenous spermine (Spm) on (A) water-soluble carbohydrates (WSC), (B) sucrose, (C) fructose, and (D) glucose content in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383584&req=5

pone.0120708.g004: The effect of exogenous spermine (Spm) on (A) water-soluble carbohydrates (WSC), (B) sucrose, (C) fructose, and (D) glucose content in leaves of white clover under drought stress.Vertical bars indicate ±SE of mean (n = 4). Different letters above columns indicate significant difference for comparison at a given day; the same number of asterisks above columns means significant differences for a particular treatment across days of drought stress don’t exist. LSD (P≤0.05). L, cv. ‘Ladino’; L+Spm, cv. ‘Ladino’ added exogenous Spm; H, cv. ‘Haifa’, H+Spm, cv. ‘Haifa’ added exogenous Spm.
Mentions: Drought stress strongly affected the accumulation of WSC in white clover leaves and content of WSC in four treatments reached to maximum after 10 d of drought stress. Compared to ‘L’ treatment, ‘L+Spm’ had 17% percent higher WSC content at the end of drought stress. ‘H+Spm’ showed significantly higher WSC content than ‘H’ treatment throughout the whole process of drought stress (Fig. 4A). In addition, sucrose contents of ‘L’ and ‘L+Spm’ treatment displayed a ~ 2-fold and ~ 3-fold increase after 5 d of drought, respectively. When drought stress lasted for 5 and 10 d, ‘L+Spm’ accumulated significantly higher sucrose content relative to ‘L’. With the development of drought stress, little change of sucrose content in cv. ‘Haifa’ was observed, while ‘H+Spm’ exhibited significantly higher sucrose content than ‘H’ at the end of drought stress (Fig. 4B). Plants of cv. ‘Haifa’ had significantly higher fructose content than plants of cv. ‘Ladino’ at the beginning. Although without statistically significant difference in fructose between ‘H+Spm’ and ‘H’ at 5 d of drought stress, ‘H+Spm’ displayed significantly higher fructose level after 10 d of water deficit relative to ‘H’ treatment. Fructose content in ‘L+Spm’ also maintained a significantly higher level under 5 and 10 d of drought stress as compared to that in ‘L’ (Fig. 4C). Exogenous Spm-treatment almost had no effect on glucose accumulation in cv. ‘Ladino’ under drought stress. However, glucose content in ‘H+Spm’ was considerably greater than that in ‘H’ under drought stress, and a peak value was visible in ‘H+Spm’ treatment at 5 d of stress (Fig. 4D).

Bottom Line: The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars.Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought.Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.

ABSTRACT
The objective of this research was to analyse whether ameliorating drought stress through exogenously applied spermine (Spm) was related to carbohydrate metabolism, dehydrins accumulation and the transcription of genes encoding dehydrins in two white clovers (drought-susceptible cv. 'Ladino' and drought-resistant cv. 'Haifa') under controlled drying conditions for 10 days. The results show that the application of Spm effectively alleviates negative effects caused by drought stress in both cultivars. Exogenous Spm led to accumulation of more water-soluble carbohydrates (WSC), sucrose, fructose and sorbitol in both cultivars under drought stress, and also significantly elevated glucose content in leaves of drought-resistant cv. 'Haifa', but had no effect on drought-susceptible cv. 'Ladino'. Accordingly, the key enzyme activities of sucrose and sorbitol metabolism changed along with the application of Spm under drought stress. Spm induced a significant increase in sucrose phosphate synthase (SPS) or sorbitol dehydrogenase (SDH) activity, but decrease in sucrose synthetase (SS) activity when two cultivars were subjected to drought. In addition, the improved accumulation of dehydrins induced by exogenous Spm coincided with three genes expression which was responsible for dehydrins synthesis. But Spm-induced transcript level of dehydrin genes increased earlier in cv. 'Ladino' than that in cv. 'Haifa'. Thus, these results suggest that ameliorating drought stress through exogenously applied Spm may be associated with increased carbohydrate accumulation and dehydrins synthesis. There are differences between drought-susceptible and -resistant white clover cultivars related to Spm regulation of WSC metabolism and dehydrins expression.

No MeSH data available.


Related in: MedlinePlus