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Urinary angiopoietin-2 is associated with albuminuria in patients with type 2 diabetes mellitus.

Chen S, Li H, Zhang C, Li Z, Wang Q, Guo J, Luo C, Wang Y - Int J Endocrinol (2015)

Bottom Line: Increased urinary Ang-2 level was positively associated with the degree of albuminuria.The levels of urinary VEGF increased in the albuminuria subgroup, though serum levels of Ang-1 and VEGF did not change.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei 430022, China.

ABSTRACT
Aims. To evaluate the levels of angiopoietin-1 (Ang-1), Ang-2, and vascular endothelial growth factor (VEGF) in serum and urine, and their association with albuminuria in patients with type 2 diabetes mellitus. Methods. In 113 type 2 diabetic patients with normoalbuminuria, microalbuminuria, and macroalbuminuria and 30 healthy controls, the levels of Ang-1, Ang-2, and VEGF in serum and urine were measured by enzyme-linked immunosorbent assay (ELISA). Results. Urinary and serum levels of Ang-2 were significantly higher in diabetic patients with normoalbuminuria than in healthy controls. Increased urinary Ang-2 level was positively associated with the degree of albuminuria. Urinary Ang-1 levels were significantly higher in normoalbuminuria patients and lower in macroalbuminuria patients than in controls. The levels of urinary VEGF increased in the albuminuria subgroup, though serum levels of Ang-1 and VEGF did not change. Urinary Ang-2 levels were correlated positively with albuminuria and negatively with glomerular filtration rate (GFR). Stepwise multiple regression analysis identified albuminuria (P < 0.001) and GFR (P = 0.001) as significant predictors of urinary Ang-2. Conclusions. Our data suggest that urinary Ang-2 is stepwise increased with renal damage in patients with type 2 diabetes mellitus and is associated with albuminuria.

No MeSH data available.


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Serum and urinary angiogenic growth factor levels in diabetic patients and controls. (a) Statistical analysis showed increased serum concentrations of Ang-2 in diabetic patients compared with controls. (b) The level of urinary Ang-2 showed a stepwise increase in diabetic patients compared to controls according to the degree of albuminuria. (c) Urinary Ang-1 level was significantly higher in the DN1 group and lower in the DN3 group when compared with control subjects. Patients in the DN1 and DN2 groups exhibited significantly higher urinary Ang-1 levels than those in the DN3 group. (d) Subjects with diabetes mellitus showed significantly higher urinary VEGF levels than control subjects. Patients were divided into DN1 (normal-albuminuria), DN2 (microalbuminuria), and DN3 (macroalbuminuria) groups. *P < 0.05 versus NC; ***P < 0.001 versus NC; ###P < 0.001 versus DN1; &&&P < 0.001 versus DN2.
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fig1: Serum and urinary angiogenic growth factor levels in diabetic patients and controls. (a) Statistical analysis showed increased serum concentrations of Ang-2 in diabetic patients compared with controls. (b) The level of urinary Ang-2 showed a stepwise increase in diabetic patients compared to controls according to the degree of albuminuria. (c) Urinary Ang-1 level was significantly higher in the DN1 group and lower in the DN3 group when compared with control subjects. Patients in the DN1 and DN2 groups exhibited significantly higher urinary Ang-1 levels than those in the DN3 group. (d) Subjects with diabetes mellitus showed significantly higher urinary VEGF levels than control subjects. Patients were divided into DN1 (normal-albuminuria), DN2 (microalbuminuria), and DN3 (macroalbuminuria) groups. *P < 0.05 versus NC; ***P < 0.001 versus NC; ###P < 0.001 versus DN1; &&&P < 0.001 versus DN2.

Mentions: Serum levels of Ang-2 were markedly increased in diabetic patients compared with values in the control group (P < 0.001; Figure 1(a)). Moreover, serum Ang-2 was significantly higher in patients with macroalbuminuria (DN3) than those in the DN1 and DN2 groups (P < 0.001; Figure 1(a)). Diabetic patients exhibited higher levels of urinary Ang-2 than controls (P < 0.001; Figure 1(b)), and urinary Ang-2 increased in a stepwise manner with increasing degrees of albuminuria in the three diabetic groups (P < 0.001; Figure 1(b)).


Urinary angiopoietin-2 is associated with albuminuria in patients with type 2 diabetes mellitus.

Chen S, Li H, Zhang C, Li Z, Wang Q, Guo J, Luo C, Wang Y - Int J Endocrinol (2015)

Serum and urinary angiogenic growth factor levels in diabetic patients and controls. (a) Statistical analysis showed increased serum concentrations of Ang-2 in diabetic patients compared with controls. (b) The level of urinary Ang-2 showed a stepwise increase in diabetic patients compared to controls according to the degree of albuminuria. (c) Urinary Ang-1 level was significantly higher in the DN1 group and lower in the DN3 group when compared with control subjects. Patients in the DN1 and DN2 groups exhibited significantly higher urinary Ang-1 levels than those in the DN3 group. (d) Subjects with diabetes mellitus showed significantly higher urinary VEGF levels than control subjects. Patients were divided into DN1 (normal-albuminuria), DN2 (microalbuminuria), and DN3 (macroalbuminuria) groups. *P < 0.05 versus NC; ***P < 0.001 versus NC; ###P < 0.001 versus DN1; &&&P < 0.001 versus DN2.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4383519&req=5

fig1: Serum and urinary angiogenic growth factor levels in diabetic patients and controls. (a) Statistical analysis showed increased serum concentrations of Ang-2 in diabetic patients compared with controls. (b) The level of urinary Ang-2 showed a stepwise increase in diabetic patients compared to controls according to the degree of albuminuria. (c) Urinary Ang-1 level was significantly higher in the DN1 group and lower in the DN3 group when compared with control subjects. Patients in the DN1 and DN2 groups exhibited significantly higher urinary Ang-1 levels than those in the DN3 group. (d) Subjects with diabetes mellitus showed significantly higher urinary VEGF levels than control subjects. Patients were divided into DN1 (normal-albuminuria), DN2 (microalbuminuria), and DN3 (macroalbuminuria) groups. *P < 0.05 versus NC; ***P < 0.001 versus NC; ###P < 0.001 versus DN1; &&&P < 0.001 versus DN2.
Mentions: Serum levels of Ang-2 were markedly increased in diabetic patients compared with values in the control group (P < 0.001; Figure 1(a)). Moreover, serum Ang-2 was significantly higher in patients with macroalbuminuria (DN3) than those in the DN1 and DN2 groups (P < 0.001; Figure 1(a)). Diabetic patients exhibited higher levels of urinary Ang-2 than controls (P < 0.001; Figure 1(b)), and urinary Ang-2 increased in a stepwise manner with increasing degrees of albuminuria in the three diabetic groups (P < 0.001; Figure 1(b)).

Bottom Line: Increased urinary Ang-2 level was positively associated with the degree of albuminuria.The levels of urinary VEGF increased in the albuminuria subgroup, though serum levels of Ang-1 and VEGF did not change.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei 430022, China.

ABSTRACT
Aims. To evaluate the levels of angiopoietin-1 (Ang-1), Ang-2, and vascular endothelial growth factor (VEGF) in serum and urine, and their association with albuminuria in patients with type 2 diabetes mellitus. Methods. In 113 type 2 diabetic patients with normoalbuminuria, microalbuminuria, and macroalbuminuria and 30 healthy controls, the levels of Ang-1, Ang-2, and VEGF in serum and urine were measured by enzyme-linked immunosorbent assay (ELISA). Results. Urinary and serum levels of Ang-2 were significantly higher in diabetic patients with normoalbuminuria than in healthy controls. Increased urinary Ang-2 level was positively associated with the degree of albuminuria. Urinary Ang-1 levels were significantly higher in normoalbuminuria patients and lower in macroalbuminuria patients than in controls. The levels of urinary VEGF increased in the albuminuria subgroup, though serum levels of Ang-1 and VEGF did not change. Urinary Ang-2 levels were correlated positively with albuminuria and negatively with glomerular filtration rate (GFR). Stepwise multiple regression analysis identified albuminuria (P < 0.001) and GFR (P = 0.001) as significant predictors of urinary Ang-2. Conclusions. Our data suggest that urinary Ang-2 is stepwise increased with renal damage in patients with type 2 diabetes mellitus and is associated with albuminuria.

No MeSH data available.


Related in: MedlinePlus