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The diversity of karyotypes and genomes within section Syllinum of the Genus Linum (Linaceae) revealed by molecular cytogenetic markers and RAPD analysis.

Bolsheva NL, Zelenin AV, Nosova IV, Amosova AV, Samatadze TE, Yurkevich OY, Melnikova NV, Zelenina DA, Volkov AA, Muravenko OV - PLoS ONE (2015)

Bottom Line: The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon.RAPD analysis did not distinguish the species except L. nodiflorum.The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

View Article: PubMed Central - PubMed

Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon. To contribute to a better understanding of the genetic diversity and species relationships in this genus, comparative studies of karyotypes and genomes of species within section Syllinum Griseb. (2n = 26, 28) were carried out. Elongated with 9-aminoacridine chromosomes of 10 species of section Syllinum were investigated by C- and DAPI/С-banding, CMA and Ag-NOR-staining, FISH with probes of rDNA and of telomere repeats. RAPD analysis was also performed. All the chromosome pairs in karyotypes of the studied species were identified. Chromosome DAPI/C-banding patterns of 28-chromosomal species were highly similar. Two of the species differed from the others in chromosomal location of rDNA sites. B chromosomes were revealed in all the 28-chromosomal species. Chromosomes of Linum nodiflorum L. (2n = 26) and the 28-chromosomal species were similar in DAPI/C-banding pattern and localization of several rDNA sites, but they differed in chromosomal size and number. The karyotype of L. nodiflorum was characterized by an intercalary site of telomere repeat, one additional 26S rDNA site and also by the absence of B chromosomes. Structural similarities between different chromosome pairs in karyotypes of the studied species were found indicating their tetraploid origin. RAPD analysis did not distinguish the species except L. nodiflorum. The species of section Syllinum probably originated from a common tetraploid ancestor. The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

No MeSH data available.


Related in: MedlinePlus

The UPGMA dendrogram of genomic relationships between species within sect. Syllinum and RAPD spectra obtained with POA10 and POY02 primers.“ind”—DNA obtained from individual plant; “sum”—bulk DNA obtained from ten individual plants.
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pone.0122015.g005: The UPGMA dendrogram of genomic relationships between species within sect. Syllinum and RAPD spectra obtained with POA10 and POY02 primers.“ind”—DNA obtained from individual plant; “sum”—bulk DNA obtained from ten individual plants.

Mentions: For estimating the value of intergenomic divergence of species from section Syllinum, a variant of RAPD method based on the use of polyacrylamide gel electrophoresis for the separation of PCR products was performed. The employed technique, which revealed a lot more electrophoretic bands in comparison with the use of agarose gel electrophoresis, allowed us to use only two PCR primers for genomic analysis. The total number of the bands amounted to 117, and 101 of which were polymorphic. The number of fragments in the individual RAPD profiles ranged from 41 to 57. The UPGMA dendrogram of individual RAPD profile similarities of the species of section Syllinum is illustrated in Fig 5. As the dendrogram shows, the species from section Syllinum formed two main clusters. The first cluster comprised only the accessions of L. nodiflorum. The second large cluster combined the 28-chromosomal species L. flavum, L. capitatum, L. campanulatum, L. thracicum, L. tauricum, L. ucrainicum and L. elegans. Their RAPD spectra were rather similar (Pearson correlation coefficient r ≥ 55%). Inside this large cluster, the DNA samples of the plants belonging to one accession often formed subclusters. Occasionally, the RAPD spectra of individual plants were comparatively more like the RAPD spectra of other species than to the spectra of the rest plants of its own accession (such as in L. thracicum accession LIN 1553). The DNA samples of the plants belonging to different accessions of one species might cluster together, for instance, it was found in three accessions of L. flavum LIN 97, LIN 99 and LIN 1527. However, they might be at a considerable distance from each other (e.g., the fourth accession of L. flavum LIN 1633 or two accessions of L. tauricum LIN 1604 and LIN 1611).


The diversity of karyotypes and genomes within section Syllinum of the Genus Linum (Linaceae) revealed by molecular cytogenetic markers and RAPD analysis.

Bolsheva NL, Zelenin AV, Nosova IV, Amosova AV, Samatadze TE, Yurkevich OY, Melnikova NV, Zelenina DA, Volkov AA, Muravenko OV - PLoS ONE (2015)

The UPGMA dendrogram of genomic relationships between species within sect. Syllinum and RAPD spectra obtained with POA10 and POY02 primers.“ind”—DNA obtained from individual plant; “sum”—bulk DNA obtained from ten individual plants.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383504&req=5

pone.0122015.g005: The UPGMA dendrogram of genomic relationships between species within sect. Syllinum and RAPD spectra obtained with POA10 and POY02 primers.“ind”—DNA obtained from individual plant; “sum”—bulk DNA obtained from ten individual plants.
Mentions: For estimating the value of intergenomic divergence of species from section Syllinum, a variant of RAPD method based on the use of polyacrylamide gel electrophoresis for the separation of PCR products was performed. The employed technique, which revealed a lot more electrophoretic bands in comparison with the use of agarose gel electrophoresis, allowed us to use only two PCR primers for genomic analysis. The total number of the bands amounted to 117, and 101 of which were polymorphic. The number of fragments in the individual RAPD profiles ranged from 41 to 57. The UPGMA dendrogram of individual RAPD profile similarities of the species of section Syllinum is illustrated in Fig 5. As the dendrogram shows, the species from section Syllinum formed two main clusters. The first cluster comprised only the accessions of L. nodiflorum. The second large cluster combined the 28-chromosomal species L. flavum, L. capitatum, L. campanulatum, L. thracicum, L. tauricum, L. ucrainicum and L. elegans. Their RAPD spectra were rather similar (Pearson correlation coefficient r ≥ 55%). Inside this large cluster, the DNA samples of the plants belonging to one accession often formed subclusters. Occasionally, the RAPD spectra of individual plants were comparatively more like the RAPD spectra of other species than to the spectra of the rest plants of its own accession (such as in L. thracicum accession LIN 1553). The DNA samples of the plants belonging to different accessions of one species might cluster together, for instance, it was found in three accessions of L. flavum LIN 97, LIN 99 and LIN 1527. However, they might be at a considerable distance from each other (e.g., the fourth accession of L. flavum LIN 1633 or two accessions of L. tauricum LIN 1604 and LIN 1611).

Bottom Line: The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon.RAPD analysis did not distinguish the species except L. nodiflorum.The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

View Article: PubMed Central - PubMed

Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon. To contribute to a better understanding of the genetic diversity and species relationships in this genus, comparative studies of karyotypes and genomes of species within section Syllinum Griseb. (2n = 26, 28) were carried out. Elongated with 9-aminoacridine chromosomes of 10 species of section Syllinum were investigated by C- and DAPI/С-banding, CMA and Ag-NOR-staining, FISH with probes of rDNA and of telomere repeats. RAPD analysis was also performed. All the chromosome pairs in karyotypes of the studied species were identified. Chromosome DAPI/C-banding patterns of 28-chromosomal species were highly similar. Two of the species differed from the others in chromosomal location of rDNA sites. B chromosomes were revealed in all the 28-chromosomal species. Chromosomes of Linum nodiflorum L. (2n = 26) and the 28-chromosomal species were similar in DAPI/C-banding pattern and localization of several rDNA sites, but they differed in chromosomal size and number. The karyotype of L. nodiflorum was characterized by an intercalary site of telomere repeat, one additional 26S rDNA site and also by the absence of B chromosomes. Structural similarities between different chromosome pairs in karyotypes of the studied species were found indicating their tetraploid origin. RAPD analysis did not distinguish the species except L. nodiflorum. The species of section Syllinum probably originated from a common tetraploid ancestor. The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

No MeSH data available.


Related in: MedlinePlus