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The diversity of karyotypes and genomes within section Syllinum of the Genus Linum (Linaceae) revealed by molecular cytogenetic markers and RAPD analysis.

Bolsheva NL, Zelenin AV, Nosova IV, Amosova AV, Samatadze TE, Yurkevich OY, Melnikova NV, Zelenina DA, Volkov AA, Muravenko OV - PLoS ONE (2015)

Bottom Line: The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon.RAPD analysis did not distinguish the species except L. nodiflorum.The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

View Article: PubMed Central - PubMed

Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon. To contribute to a better understanding of the genetic diversity and species relationships in this genus, comparative studies of karyotypes and genomes of species within section Syllinum Griseb. (2n = 26, 28) were carried out. Elongated with 9-aminoacridine chromosomes of 10 species of section Syllinum were investigated by C- and DAPI/С-banding, CMA and Ag-NOR-staining, FISH with probes of rDNA and of telomere repeats. RAPD analysis was also performed. All the chromosome pairs in karyotypes of the studied species were identified. Chromosome DAPI/C-banding patterns of 28-chromosomal species were highly similar. Two of the species differed from the others in chromosomal location of rDNA sites. B chromosomes were revealed in all the 28-chromosomal species. Chromosomes of Linum nodiflorum L. (2n = 26) and the 28-chromosomal species were similar in DAPI/C-banding pattern and localization of several rDNA sites, but they differed in chromosomal size and number. The karyotype of L. nodiflorum was characterized by an intercalary site of telomere repeat, one additional 26S rDNA site and also by the absence of B chromosomes. Structural similarities between different chromosome pairs in karyotypes of the studied species were found indicating their tetraploid origin. RAPD analysis did not distinguish the species except L. nodiflorum. The species of section Syllinum probably originated from a common tetraploid ancestor. The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

No MeSH data available.


Related in: MedlinePlus

AgNOR staining and localization of telomeric repeats.AgNOR staining.of metaphase chromosomes of L. flavum (A) and L. nodiflorum (C). Superposition of AgNOR (dark) and DAPI staining (blue) of the same metaphase plates (B, D). Localization of telomeric repeats (green) on metaphase plates of L. flavum (E) and L. nodiflorum (G) revealed by FISH. Inverted DAPI staining (grey) of the same metaphase plates (F, H). Arrows point to intercalary loci of telomere repeats on chromosomes 3 of L. nodiflorum. B—B-chromosomes. Scale bar—5 μm.
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pone.0122015.g004: AgNOR staining and localization of telomeric repeats.AgNOR staining.of metaphase chromosomes of L. flavum (A) and L. nodiflorum (C). Superposition of AgNOR (dark) and DAPI staining (blue) of the same metaphase plates (B, D). Localization of telomeric repeats (green) on metaphase plates of L. flavum (E) and L. nodiflorum (G) revealed by FISH. Inverted DAPI staining (grey) of the same metaphase plates (F, H). Arrows point to intercalary loci of telomere repeats on chromosomes 3 of L. nodiflorum. B—B-chromosomes. Scale bar—5 μm.

Mentions: For investigating the level of transcriptional activity of the revealed rDNA sites, the examined species were studied using Ag-NOR staining. It was found that the secondary constriction of chromosome 7 in karyotypes of L. flavum, L. campanulatum, L. elegans, L. tauricum, L. thracicum, L. capitatum, L. dolomiticum, L. ucrainicum and L. czernjajevii was stained by silver nitrate. At the same time, B chromosomes were not stained by silver nitrate (Figs. 4A, 4B) suggesting that they comprised no transcriptionally active rRNA genes. In metaphase of L. nodiflorum, two pairs of chromosomes were silver stained (Figs. 4C, 4D).


The diversity of karyotypes and genomes within section Syllinum of the Genus Linum (Linaceae) revealed by molecular cytogenetic markers and RAPD analysis.

Bolsheva NL, Zelenin AV, Nosova IV, Amosova AV, Samatadze TE, Yurkevich OY, Melnikova NV, Zelenina DA, Volkov AA, Muravenko OV - PLoS ONE (2015)

AgNOR staining and localization of telomeric repeats.AgNOR staining.of metaphase chromosomes of L. flavum (A) and L. nodiflorum (C). Superposition of AgNOR (dark) and DAPI staining (blue) of the same metaphase plates (B, D). Localization of telomeric repeats (green) on metaphase plates of L. flavum (E) and L. nodiflorum (G) revealed by FISH. Inverted DAPI staining (grey) of the same metaphase plates (F, H). Arrows point to intercalary loci of telomere repeats on chromosomes 3 of L. nodiflorum. B—B-chromosomes. Scale bar—5 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383504&req=5

pone.0122015.g004: AgNOR staining and localization of telomeric repeats.AgNOR staining.of metaphase chromosomes of L. flavum (A) and L. nodiflorum (C). Superposition of AgNOR (dark) and DAPI staining (blue) of the same metaphase plates (B, D). Localization of telomeric repeats (green) on metaphase plates of L. flavum (E) and L. nodiflorum (G) revealed by FISH. Inverted DAPI staining (grey) of the same metaphase plates (F, H). Arrows point to intercalary loci of telomere repeats on chromosomes 3 of L. nodiflorum. B—B-chromosomes. Scale bar—5 μm.
Mentions: For investigating the level of transcriptional activity of the revealed rDNA sites, the examined species were studied using Ag-NOR staining. It was found that the secondary constriction of chromosome 7 in karyotypes of L. flavum, L. campanulatum, L. elegans, L. tauricum, L. thracicum, L. capitatum, L. dolomiticum, L. ucrainicum and L. czernjajevii was stained by silver nitrate. At the same time, B chromosomes were not stained by silver nitrate (Figs. 4A, 4B) suggesting that they comprised no transcriptionally active rRNA genes. In metaphase of L. nodiflorum, two pairs of chromosomes were silver stained (Figs. 4C, 4D).

Bottom Line: The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon.RAPD analysis did not distinguish the species except L. nodiflorum.The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

View Article: PubMed Central - PubMed

Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon. To contribute to a better understanding of the genetic diversity and species relationships in this genus, comparative studies of karyotypes and genomes of species within section Syllinum Griseb. (2n = 26, 28) were carried out. Elongated with 9-aminoacridine chromosomes of 10 species of section Syllinum were investigated by C- and DAPI/С-banding, CMA and Ag-NOR-staining, FISH with probes of rDNA and of telomere repeats. RAPD analysis was also performed. All the chromosome pairs in karyotypes of the studied species were identified. Chromosome DAPI/C-banding patterns of 28-chromosomal species were highly similar. Two of the species differed from the others in chromosomal location of rDNA sites. B chromosomes were revealed in all the 28-chromosomal species. Chromosomes of Linum nodiflorum L. (2n = 26) and the 28-chromosomal species were similar in DAPI/C-banding pattern and localization of several rDNA sites, but they differed in chromosomal size and number. The karyotype of L. nodiflorum was characterized by an intercalary site of telomere repeat, one additional 26S rDNA site and also by the absence of B chromosomes. Structural similarities between different chromosome pairs in karyotypes of the studied species were found indicating their tetraploid origin. RAPD analysis did not distinguish the species except L. nodiflorum. The species of section Syllinum probably originated from a common tetraploid ancestor. The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

No MeSH data available.


Related in: MedlinePlus