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The diversity of karyotypes and genomes within section Syllinum of the Genus Linum (Linaceae) revealed by molecular cytogenetic markers and RAPD analysis.

Bolsheva NL, Zelenin AV, Nosova IV, Amosova AV, Samatadze TE, Yurkevich OY, Melnikova NV, Zelenina DA, Volkov AA, Muravenko OV - PLoS ONE (2015)

Bottom Line: The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon.RAPD analysis did not distinguish the species except L. nodiflorum.The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

View Article: PubMed Central - PubMed

Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon. To contribute to a better understanding of the genetic diversity and species relationships in this genus, comparative studies of karyotypes and genomes of species within section Syllinum Griseb. (2n = 26, 28) were carried out. Elongated with 9-aminoacridine chromosomes of 10 species of section Syllinum were investigated by C- and DAPI/С-banding, CMA and Ag-NOR-staining, FISH with probes of rDNA and of telomere repeats. RAPD analysis was also performed. All the chromosome pairs in karyotypes of the studied species were identified. Chromosome DAPI/C-banding patterns of 28-chromosomal species were highly similar. Two of the species differed from the others in chromosomal location of rDNA sites. B chromosomes were revealed in all the 28-chromosomal species. Chromosomes of Linum nodiflorum L. (2n = 26) and the 28-chromosomal species were similar in DAPI/C-banding pattern and localization of several rDNA sites, but they differed in chromosomal size and number. The karyotype of L. nodiflorum was characterized by an intercalary site of telomere repeat, one additional 26S rDNA site and also by the absence of B chromosomes. Structural similarities between different chromosome pairs in karyotypes of the studied species were found indicating their tetraploid origin. RAPD analysis did not distinguish the species except L. nodiflorum. The species of section Syllinum probably originated from a common tetraploid ancestor. The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

No MeSH data available.


Related in: MedlinePlus

The karyotypes and chromosome idiograms of species from sect. Syllinum.Inverted DAPI/C-banding pattern (grey). Chromosomes are numbered according to their sizes. Localization of 26S (green) and 5S (red) rDNA revealed by FISH (coloured).
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pone.0122015.g002: The karyotypes and chromosome idiograms of species from sect. Syllinum.Inverted DAPI/C-banding pattern (grey). Chromosomes are numbered according to their sizes. Localization of 26S (green) and 5S (red) rDNA revealed by FISH (coloured).

Mentions: DAPI staining carried out after FISH, which included denaturation and renaturation of chromosomal DNA, revealed C-banding-like patterns (DAPI/C-banding patterns) in karyotypes of the members of section Syllinum. Optimization of a hydrolysis procedure (see ‘Material and Methods‘ section) significantly improved the quality of chromosome spreads as well as the resolution of DAPI/C-banding patterns in karyotypes compared with the usual C-banding technique. The combination of DAPI/C-banding and FISH with 26S and 5S rDNA probes allowed us to identify all the individual chromosomes in karyotypes of the species from section Syllinum (Fig 2, S1 and S2 Tables).


The diversity of karyotypes and genomes within section Syllinum of the Genus Linum (Linaceae) revealed by molecular cytogenetic markers and RAPD analysis.

Bolsheva NL, Zelenin AV, Nosova IV, Amosova AV, Samatadze TE, Yurkevich OY, Melnikova NV, Zelenina DA, Volkov AA, Muravenko OV - PLoS ONE (2015)

The karyotypes and chromosome idiograms of species from sect. Syllinum.Inverted DAPI/C-banding pattern (grey). Chromosomes are numbered according to their sizes. Localization of 26S (green) and 5S (red) rDNA revealed by FISH (coloured).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383504&req=5

pone.0122015.g002: The karyotypes and chromosome idiograms of species from sect. Syllinum.Inverted DAPI/C-banding pattern (grey). Chromosomes are numbered according to their sizes. Localization of 26S (green) and 5S (red) rDNA revealed by FISH (coloured).
Mentions: DAPI staining carried out after FISH, which included denaturation and renaturation of chromosomal DNA, revealed C-banding-like patterns (DAPI/C-banding patterns) in karyotypes of the members of section Syllinum. Optimization of a hydrolysis procedure (see ‘Material and Methods‘ section) significantly improved the quality of chromosome spreads as well as the resolution of DAPI/C-banding patterns in karyotypes compared with the usual C-banding technique. The combination of DAPI/C-banding and FISH with 26S and 5S rDNA probes allowed us to identify all the individual chromosomes in karyotypes of the species from section Syllinum (Fig 2, S1 and S2 Tables).

Bottom Line: The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon.RAPD analysis did not distinguish the species except L. nodiflorum.The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

View Article: PubMed Central - PubMed

Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.

ABSTRACT
The wide variation in chromosome number found in species of the genus Linum (2n = 16, 18, 20, 26, 28, 30, 32, 36, 42, 72, 84) indicates that chromosomal mutations have played an important role in the speciation of this taxon. To contribute to a better understanding of the genetic diversity and species relationships in this genus, comparative studies of karyotypes and genomes of species within section Syllinum Griseb. (2n = 26, 28) were carried out. Elongated with 9-aminoacridine chromosomes of 10 species of section Syllinum were investigated by C- and DAPI/С-banding, CMA and Ag-NOR-staining, FISH with probes of rDNA and of telomere repeats. RAPD analysis was also performed. All the chromosome pairs in karyotypes of the studied species were identified. Chromosome DAPI/C-banding patterns of 28-chromosomal species were highly similar. Two of the species differed from the others in chromosomal location of rDNA sites. B chromosomes were revealed in all the 28-chromosomal species. Chromosomes of Linum nodiflorum L. (2n = 26) and the 28-chromosomal species were similar in DAPI/C-banding pattern and localization of several rDNA sites, but they differed in chromosomal size and number. The karyotype of L. nodiflorum was characterized by an intercalary site of telomere repeat, one additional 26S rDNA site and also by the absence of B chromosomes. Structural similarities between different chromosome pairs in karyotypes of the studied species were found indicating their tetraploid origin. RAPD analysis did not distinguish the species except L. nodiflorum. The species of section Syllinum probably originated from a common tetraploid ancestor. The 28-chromosomal species were closely related, but L. nodiflorum diverged significantly from the rest of the species probably due to chromosomal rearrangements occurring during evolution.

No MeSH data available.


Related in: MedlinePlus