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The intrauterine and nursing period is a window of susceptibility for development of obesity and intestinal tumorigenesis by a high fat diet in Min/+ mice as adults.

Ngo HT, Hetland RB, Steffensen IL - J Obes (2015)

Bottom Line: We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice.In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did.The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

View Article: PubMed Central - PubMed

Affiliation: Department of Food, Water and Cosmetics, Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, 0403 Oslo, Norway.

ABSTRACT
We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice. The mice were given a 10% fat diet throughout life (negative control) or a 45% fat diet in utero, during nursing, during both in utero and nursing, during adult life, or during their whole life-span, and terminated at 11 weeks for tumorigenesis (Min/+) or 23 weeks for obesogenic effect (wild-type). Body weight at 11 weeks was increased after a 45% fat diet during nursing, during both in utero and nursing, and throughout life, but had normalized at 23 weeks. In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did. However, a 45% fat diet during nursing or during in utero and nursing increased the number of small intestinal tumors. So did exposures to a 45% fat diet in adult life or throughout life, but without increasing the tumor numbers further. The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

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Related in: MedlinePlus

Body weight development (in gram) of female and male mice of all treatment groups is illustrated for (a) Min/+ and (b) wild-type mice from age 3-4 days to 11 weeks, and for (c) wild-type mice from age 12 to 23 weeks. The open white symbols are for untreated female mice; 10+10+10 (○), 45+10+10 (△), 10+45+10 (▽), 45+45+10 (□), 10+10+45 (◊), 45+45+45 (open hexagon), and the same filled black symbols are for untreated male mice. The PhIP-treated groups are marked with grey symbols; 45+45+10 PhIP (○) and 45+45+45 PhIP (□) in females, and 45+45+10 PhIP (△), 45+45+45 PhIP (▽) in males. The experimental groups are as explained in the legend to Figure 1. n = 20–46.
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fig5: Body weight development (in gram) of female and male mice of all treatment groups is illustrated for (a) Min/+ and (b) wild-type mice from age 3-4 days to 11 weeks, and for (c) wild-type mice from age 12 to 23 weeks. The open white symbols are for untreated female mice; 10+10+10 (○), 45+10+10 (△), 10+45+10 (▽), 45+45+10 (□), 10+10+45 (◊), 45+45+45 (open hexagon), and the same filled black symbols are for untreated male mice. The PhIP-treated groups are marked with grey symbols; 45+45+10 PhIP (○) and 45+45+45 PhIP (□) in females, and 45+45+10 PhIP (△), 45+45+45 PhIP (▽) in males. The experimental groups are as explained in the legend to Figure 1. n = 20–46.

Mentions: Body weight development (in gram) for both female and male Min/+ (Figure 5(a)) and wild-type (Figure 5(b)) mice of all treatment groups is shown from age 3-4 days to 11 weeks. The body weight development over time of the mice offspring was evaluated statistically as area under the curve (AUC) from day 3-4 to week 11 for Min/+ (Figures 6(a) and 6(b)) and wild-type (Figures 6(c) and 6(d)) mice for each dietary group. The Min/+ mice had a lower AUC compared with the wild-type mice in both females and males, and in mice both with and without PhIP treatment (P < 0.001 for all comparisons). Both Min/+ and wild-type male mice had larger AUC than females (P < 0.001 both comparisons), which was apparent in all dietary groups (P < 0.001 for all comparisons). Min/+ mice exposed to PhIP had lower body weight than mice not exposed to PhIP (P = 0.027), but PhIP did not affect the body weight in the wild-type mice.


The intrauterine and nursing period is a window of susceptibility for development of obesity and intestinal tumorigenesis by a high fat diet in Min/+ mice as adults.

Ngo HT, Hetland RB, Steffensen IL - J Obes (2015)

Body weight development (in gram) of female and male mice of all treatment groups is illustrated for (a) Min/+ and (b) wild-type mice from age 3-4 days to 11 weeks, and for (c) wild-type mice from age 12 to 23 weeks. The open white symbols are for untreated female mice; 10+10+10 (○), 45+10+10 (△), 10+45+10 (▽), 45+45+10 (□), 10+10+45 (◊), 45+45+45 (open hexagon), and the same filled black symbols are for untreated male mice. The PhIP-treated groups are marked with grey symbols; 45+45+10 PhIP (○) and 45+45+45 PhIP (□) in females, and 45+45+10 PhIP (△), 45+45+45 PhIP (▽) in males. The experimental groups are as explained in the legend to Figure 1. n = 20–46.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383426&req=5

fig5: Body weight development (in gram) of female and male mice of all treatment groups is illustrated for (a) Min/+ and (b) wild-type mice from age 3-4 days to 11 weeks, and for (c) wild-type mice from age 12 to 23 weeks. The open white symbols are for untreated female mice; 10+10+10 (○), 45+10+10 (△), 10+45+10 (▽), 45+45+10 (□), 10+10+45 (◊), 45+45+45 (open hexagon), and the same filled black symbols are for untreated male mice. The PhIP-treated groups are marked with grey symbols; 45+45+10 PhIP (○) and 45+45+45 PhIP (□) in females, and 45+45+10 PhIP (△), 45+45+45 PhIP (▽) in males. The experimental groups are as explained in the legend to Figure 1. n = 20–46.
Mentions: Body weight development (in gram) for both female and male Min/+ (Figure 5(a)) and wild-type (Figure 5(b)) mice of all treatment groups is shown from age 3-4 days to 11 weeks. The body weight development over time of the mice offspring was evaluated statistically as area under the curve (AUC) from day 3-4 to week 11 for Min/+ (Figures 6(a) and 6(b)) and wild-type (Figures 6(c) and 6(d)) mice for each dietary group. The Min/+ mice had a lower AUC compared with the wild-type mice in both females and males, and in mice both with and without PhIP treatment (P < 0.001 for all comparisons). Both Min/+ and wild-type male mice had larger AUC than females (P < 0.001 both comparisons), which was apparent in all dietary groups (P < 0.001 for all comparisons). Min/+ mice exposed to PhIP had lower body weight than mice not exposed to PhIP (P = 0.027), but PhIP did not affect the body weight in the wild-type mice.

Bottom Line: We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice.In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did.The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

View Article: PubMed Central - PubMed

Affiliation: Department of Food, Water and Cosmetics, Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, 0403 Oslo, Norway.

ABSTRACT
We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice. The mice were given a 10% fat diet throughout life (negative control) or a 45% fat diet in utero, during nursing, during both in utero and nursing, during adult life, or during their whole life-span, and terminated at 11 weeks for tumorigenesis (Min/+) or 23 weeks for obesogenic effect (wild-type). Body weight at 11 weeks was increased after a 45% fat diet during nursing, during both in utero and nursing, and throughout life, but had normalized at 23 weeks. In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did. However, a 45% fat diet during nursing or during in utero and nursing increased the number of small intestinal tumors. So did exposures to a 45% fat diet in adult life or throughout life, but without increasing the tumor numbers further. The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

Show MeSH
Related in: MedlinePlus