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The intrauterine and nursing period is a window of susceptibility for development of obesity and intestinal tumorigenesis by a high fat diet in Min/+ mice as adults.

Ngo HT, Hetland RB, Steffensen IL - J Obes (2015)

Bottom Line: We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice.In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did.The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

View Article: PubMed Central - PubMed

Affiliation: Department of Food, Water and Cosmetics, Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, 0403 Oslo, Norway.

ABSTRACT
We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice. The mice were given a 10% fat diet throughout life (negative control) or a 45% fat diet in utero, during nursing, during both in utero and nursing, during adult life, or during their whole life-span, and terminated at 11 weeks for tumorigenesis (Min/+) or 23 weeks for obesogenic effect (wild-type). Body weight at 11 weeks was increased after a 45% fat diet during nursing, during both in utero and nursing, and throughout life, but had normalized at 23 weeks. In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did. However, a 45% fat diet during nursing or during in utero and nursing increased the number of small intestinal tumors. So did exposures to a 45% fat diet in adult life or throughout life, but without increasing the tumor numbers further. The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

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Related in: MedlinePlus

The feed intake of the dams during (a) pregnancy, that is, from mating to the end of week 3 of pregnancy (n = 37–119), and (b) the three week lactation period (n = 12–69), recorded as gram diet per gram body weight per week (mean ± SD). During pregnancy, the dams were given either a 10% fat (white columns) or a 45% fat diet (black columns). The data for the lactation period were stratified according to the four different combinations of the 10% fat or 45% fat diet during pregnancy and the 10% fat or 45% fat diets during the lactation period; 10+10+ (white columns), 45+10+ (light grey columns), 10+45+ (dark grey columns), 45+45+ (black columns), as explained in the legend to Figure 1. (a) a, bSignificantly higher with a 10% fat diet versus a 45% fat diet within the same week. (b) a, b, cSignificantly higher with a 10% fat diet during both pregnancy and nursing periods versus a 45% fat diet in the same periods within the same week. (b) d, eSignificantly higher with a 45% fat diet during pregnancy and a 10% fat diet during nursing versus a 45% fat diet in both periods within the same week.
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fig2: The feed intake of the dams during (a) pregnancy, that is, from mating to the end of week 3 of pregnancy (n = 37–119), and (b) the three week lactation period (n = 12–69), recorded as gram diet per gram body weight per week (mean ± SD). During pregnancy, the dams were given either a 10% fat (white columns) or a 45% fat diet (black columns). The data for the lactation period were stratified according to the four different combinations of the 10% fat or 45% fat diet during pregnancy and the 10% fat or 45% fat diets during the lactation period; 10+10+ (white columns), 45+10+ (light grey columns), 10+45+ (dark grey columns), 45+45+ (black columns), as explained in the legend to Figure 1. (a) a, bSignificantly higher with a 10% fat diet versus a 45% fat diet within the same week. (b) a, b, cSignificantly higher with a 10% fat diet during both pregnancy and nursing periods versus a 45% fat diet in the same periods within the same week. (b) d, eSignificantly higher with a 45% fat diet during pregnancy and a 10% fat diet during nursing versus a 45% fat diet in both periods within the same week.

Mentions: The feed intake of the mice dams was recorded as gram feed per gram body weight per week for each of the three weeks of pregnancy (Figure 2(a)). The dams had a significantly higher feed intake per gram body weight in both week 1 and 2 compared with week 3, of both the 10% fat and 45% fat diets (P < 0.001 for all comparisons), and in week 1 compared with week 2 for the 45% fat diet (P < 0.001). The intake of feed per gram body weight was higher for the 10% fat diet than the 45% fat diet for all three weeks together, and for weeks 2 and 3 separately (P < 0.001 for all comparisons).


The intrauterine and nursing period is a window of susceptibility for development of obesity and intestinal tumorigenesis by a high fat diet in Min/+ mice as adults.

Ngo HT, Hetland RB, Steffensen IL - J Obes (2015)

The feed intake of the dams during (a) pregnancy, that is, from mating to the end of week 3 of pregnancy (n = 37–119), and (b) the three week lactation period (n = 12–69), recorded as gram diet per gram body weight per week (mean ± SD). During pregnancy, the dams were given either a 10% fat (white columns) or a 45% fat diet (black columns). The data for the lactation period were stratified according to the four different combinations of the 10% fat or 45% fat diet during pregnancy and the 10% fat or 45% fat diets during the lactation period; 10+10+ (white columns), 45+10+ (light grey columns), 10+45+ (dark grey columns), 45+45+ (black columns), as explained in the legend to Figure 1. (a) a, bSignificantly higher with a 10% fat diet versus a 45% fat diet within the same week. (b) a, b, cSignificantly higher with a 10% fat diet during both pregnancy and nursing periods versus a 45% fat diet in the same periods within the same week. (b) d, eSignificantly higher with a 45% fat diet during pregnancy and a 10% fat diet during nursing versus a 45% fat diet in both periods within the same week.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4383426&req=5

fig2: The feed intake of the dams during (a) pregnancy, that is, from mating to the end of week 3 of pregnancy (n = 37–119), and (b) the three week lactation period (n = 12–69), recorded as gram diet per gram body weight per week (mean ± SD). During pregnancy, the dams were given either a 10% fat (white columns) or a 45% fat diet (black columns). The data for the lactation period were stratified according to the four different combinations of the 10% fat or 45% fat diet during pregnancy and the 10% fat or 45% fat diets during the lactation period; 10+10+ (white columns), 45+10+ (light grey columns), 10+45+ (dark grey columns), 45+45+ (black columns), as explained in the legend to Figure 1. (a) a, bSignificantly higher with a 10% fat diet versus a 45% fat diet within the same week. (b) a, b, cSignificantly higher with a 10% fat diet during both pregnancy and nursing periods versus a 45% fat diet in the same periods within the same week. (b) d, eSignificantly higher with a 45% fat diet during pregnancy and a 10% fat diet during nursing versus a 45% fat diet in both periods within the same week.
Mentions: The feed intake of the mice dams was recorded as gram feed per gram body weight per week for each of the three weeks of pregnancy (Figure 2(a)). The dams had a significantly higher feed intake per gram body weight in both week 1 and 2 compared with week 3, of both the 10% fat and 45% fat diets (P < 0.001 for all comparisons), and in week 1 compared with week 2 for the 45% fat diet (P < 0.001). The intake of feed per gram body weight was higher for the 10% fat diet than the 45% fat diet for all three weeks together, and for weeks 2 and 3 separately (P < 0.001 for all comparisons).

Bottom Line: We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice.In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did.The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

View Article: PubMed Central - PubMed

Affiliation: Department of Food, Water and Cosmetics, Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, 0403 Oslo, Norway.

ABSTRACT
We studied how obesogenic conditions during various life periods affected obesity and intestinal tumorigenesis in adult C57BL/6J-Min (multiple intestinal neoplasia)/+ mice. The mice were given a 10% fat diet throughout life (negative control) or a 45% fat diet in utero, during nursing, during both in utero and nursing, during adult life, or during their whole life-span, and terminated at 11 weeks for tumorigenesis (Min/+) or 23 weeks for obesogenic effect (wild-type). Body weight at 11 weeks was increased after a 45% fat diet during nursing, during both in utero and nursing, and throughout life, but had normalized at 23 weeks. In the glucose tolerance test, the early exposure to a 45% fat diet in utero, during nursing, or during both in utero and nursing, did not affect blood glucose, whereas a 45% fat diet given to adults or throughout life did. However, a 45% fat diet during nursing or during in utero and nursing increased the number of small intestinal tumors. So did exposures to a 45% fat diet in adult life or throughout life, but without increasing the tumor numbers further. The intrauterine and nursing period is a window of susceptibility for dietary fat-induced obesity and intestinal tumor development.

Show MeSH
Related in: MedlinePlus