Limits...
Classical olfactory conditioning in the oriental fruit fly, Bactrocera dorsalis.

Liu JL, Chen XY, Zeng XN - PLoS ONE (2015)

Bottom Line: Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant.The acquisition of the proboscis extension reflex was used as the criterion for conditioning.A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong, China.

ABSTRACT
The oriental fruit fly, Bactrocera dorsalis, is a serious pest of fruits and vegetables. Methyl eugenol (ME), a male attractant, is used to against this fly by mass trapping. Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant. To collect the behavioral evidence, studies on the capability of this fly for olfactory learning are necessary. We investigated olfactory learning in male flies with a classical olfactory conditioning procedure using restrained individuals under laboratory conditions. The acquisition of the proboscis extension reflex was used as the criterion for conditioning. A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired. We also found that the conditioning performance was influenced by the odor concentration, intertrial interval, and starvation time. A slight sensitization elicited by imbibing sucrose was observed. These results indicate that oriental fruit flies have a high capacity to form an olfactory memory as a result of classical conditioning.

No MeSH data available.


Related in: MedlinePlus

Effects of intertrial intervals on conditioning.Percentage of B. dorsalis showed conditioned response to the CS (% Conditioned response) in 6 conditioning trials with intertrial intervals of 1, 3, 5, 10, and 15 min (n = 59, 64, 64, 58, and 63, respectively). Different letters indicate significant pairwise differences in learning performance (Scheffé test after the Kruskal-Wallis test).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4383412&req=5

pone.0122155.g005: Effects of intertrial intervals on conditioning.Percentage of B. dorsalis showed conditioned response to the CS (% Conditioned response) in 6 conditioning trials with intertrial intervals of 1, 3, 5, 10, and 15 min (n = 59, 64, 64, 58, and 63, respectively). Different letters indicate significant pairwise differences in learning performance (Scheffé test after the Kruskal-Wallis test).

Mentions: The effect of ITI on conditioning was investigated in the training of five groups of flies in six conditioning trials with ITIs of 1, 3, 5, 10, and 15 min, respectively. In these groups, the percentages of conditioned response increased significantly with successive trials (1 min: Q = 10; 3 min: Q = 30.02; 5 min: Q = 34.8; 10 min: Q = 16.79; 15 min: Q = 13.7; all df = 4, p < 0.05; Fig 5), reaching a maximum of 68.9%, 48.4%, 64.1%, 77.6%, and 63.6%, at the second trial in the 1-min group, the fourth trial in the 3-min group, the fifth trial in the 5-min group, the fourth trial in the 10-min group, and the fifth trial in the 15-min group, respectively.


Classical olfactory conditioning in the oriental fruit fly, Bactrocera dorsalis.

Liu JL, Chen XY, Zeng XN - PLoS ONE (2015)

Effects of intertrial intervals on conditioning.Percentage of B. dorsalis showed conditioned response to the CS (% Conditioned response) in 6 conditioning trials with intertrial intervals of 1, 3, 5, 10, and 15 min (n = 59, 64, 64, 58, and 63, respectively). Different letters indicate significant pairwise differences in learning performance (Scheffé test after the Kruskal-Wallis test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383412&req=5

pone.0122155.g005: Effects of intertrial intervals on conditioning.Percentage of B. dorsalis showed conditioned response to the CS (% Conditioned response) in 6 conditioning trials with intertrial intervals of 1, 3, 5, 10, and 15 min (n = 59, 64, 64, 58, and 63, respectively). Different letters indicate significant pairwise differences in learning performance (Scheffé test after the Kruskal-Wallis test).
Mentions: The effect of ITI on conditioning was investigated in the training of five groups of flies in six conditioning trials with ITIs of 1, 3, 5, 10, and 15 min, respectively. In these groups, the percentages of conditioned response increased significantly with successive trials (1 min: Q = 10; 3 min: Q = 30.02; 5 min: Q = 34.8; 10 min: Q = 16.79; 15 min: Q = 13.7; all df = 4, p < 0.05; Fig 5), reaching a maximum of 68.9%, 48.4%, 64.1%, 77.6%, and 63.6%, at the second trial in the 1-min group, the fourth trial in the 3-min group, the fifth trial in the 5-min group, the fourth trial in the 10-min group, and the fifth trial in the 15-min group, respectively.

Bottom Line: Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant.The acquisition of the proboscis extension reflex was used as the criterion for conditioning.A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong, China.

ABSTRACT
The oriental fruit fly, Bactrocera dorsalis, is a serious pest of fruits and vegetables. Methyl eugenol (ME), a male attractant, is used to against this fly by mass trapping. Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant. To collect the behavioral evidence, studies on the capability of this fly for olfactory learning are necessary. We investigated olfactory learning in male flies with a classical olfactory conditioning procedure using restrained individuals under laboratory conditions. The acquisition of the proboscis extension reflex was used as the criterion for conditioning. A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired. We also found that the conditioning performance was influenced by the odor concentration, intertrial interval, and starvation time. A slight sensitization elicited by imbibing sucrose was observed. These results indicate that oriental fruit flies have a high capacity to form an olfactory memory as a result of classical conditioning.

No MeSH data available.


Related in: MedlinePlus