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Classical olfactory conditioning in the oriental fruit fly, Bactrocera dorsalis.

Liu JL, Chen XY, Zeng XN - PLoS ONE (2015)

Bottom Line: Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant.The acquisition of the proboscis extension reflex was used as the criterion for conditioning.A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong, China.

ABSTRACT
The oriental fruit fly, Bactrocera dorsalis, is a serious pest of fruits and vegetables. Methyl eugenol (ME), a male attractant, is used to against this fly by mass trapping. Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant. To collect the behavioral evidence, studies on the capability of this fly for olfactory learning are necessary. We investigated olfactory learning in male flies with a classical olfactory conditioning procedure using restrained individuals under laboratory conditions. The acquisition of the proboscis extension reflex was used as the criterion for conditioning. A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired. We also found that the conditioning performance was influenced by the odor concentration, intertrial interval, and starvation time. A slight sensitization elicited by imbibing sucrose was observed. These results indicate that oriental fruit flies have a high capacity to form an olfactory memory as a result of classical conditioning.

No MeSH data available.


Related in: MedlinePlus

Olfactory conditioning of the proboscis extension response in B. dorsalis.Percentages of B. dorsalis showed conditioned response to the CS (% conditioned response) in 6 trials with paired training (n = 58) and unpaired training (n = 55). The results of statistical comparison paired and unpaired conditioning in each training trial are shown as asterisks (G—test, **p < 0.01)
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pone.0122155.g002: Olfactory conditioning of the proboscis extension response in B. dorsalis.Percentages of B. dorsalis showed conditioned response to the CS (% conditioned response) in 6 trials with paired training (n = 58) and unpaired training (n = 55). The results of statistical comparison paired and unpaired conditioning in each training trial are shown as asterisks (G—test, **p < 0.01)

Mentions: We first investigated whether the frequency of conditioned response increased during CS presentations with paired training. The first trial was excluded from analysis because it was designed to have a response, and response first trial in other experiments also was excluded from analysis. The percentage of conditioned response to the CS significantly increased with the subsequent five successive conditioning trials (Cochran’s test Q = 15.77 df = 4, p < 0.01; Fig 2) and reached a maximum of 77.6% at the fourth trial (after three training trials). Of the 58 flies, 7 failed to show the conditioned response to the peppermint odor.


Classical olfactory conditioning in the oriental fruit fly, Bactrocera dorsalis.

Liu JL, Chen XY, Zeng XN - PLoS ONE (2015)

Olfactory conditioning of the proboscis extension response in B. dorsalis.Percentages of B. dorsalis showed conditioned response to the CS (% conditioned response) in 6 trials with paired training (n = 58) and unpaired training (n = 55). The results of statistical comparison paired and unpaired conditioning in each training trial are shown as asterisks (G—test, **p < 0.01)
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4383412&req=5

pone.0122155.g002: Olfactory conditioning of the proboscis extension response in B. dorsalis.Percentages of B. dorsalis showed conditioned response to the CS (% conditioned response) in 6 trials with paired training (n = 58) and unpaired training (n = 55). The results of statistical comparison paired and unpaired conditioning in each training trial are shown as asterisks (G—test, **p < 0.01)
Mentions: We first investigated whether the frequency of conditioned response increased during CS presentations with paired training. The first trial was excluded from analysis because it was designed to have a response, and response first trial in other experiments also was excluded from analysis. The percentage of conditioned response to the CS significantly increased with the subsequent five successive conditioning trials (Cochran’s test Q = 15.77 df = 4, p < 0.01; Fig 2) and reached a maximum of 77.6% at the fourth trial (after three training trials). Of the 58 flies, 7 failed to show the conditioned response to the peppermint odor.

Bottom Line: Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant.The acquisition of the proboscis extension reflex was used as the criterion for conditioning.A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong, China.

ABSTRACT
The oriental fruit fly, Bactrocera dorsalis, is a serious pest of fruits and vegetables. Methyl eugenol (ME), a male attractant, is used to against this fly by mass trapping. Control effect may be influenced by learning, which could modify the olfactory response of the fly to this attractant. To collect the behavioral evidence, studies on the capability of this fly for olfactory learning are necessary. We investigated olfactory learning in male flies with a classical olfactory conditioning procedure using restrained individuals under laboratory conditions. The acquisition of the proboscis extension reflex was used as the criterion for conditioning. A high conditioned response level was found in oriental fruit flies when an odor was presented in paired association with a sucrose reward but not when the odor and sucrose were presented unpaired. We also found that the conditioning performance was influenced by the odor concentration, intertrial interval, and starvation time. A slight sensitization elicited by imbibing sucrose was observed. These results indicate that oriental fruit flies have a high capacity to form an olfactory memory as a result of classical conditioning.

No MeSH data available.


Related in: MedlinePlus