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Construction of a CXCL12-KDEL fusion gene to inhibit head and neck squamous cell carcinoma metastasis by intracellular sequestration of CXCR4.

Zhang W, Wang X, Yue K, Liu S, Liu X - Biomed Res Int (2015)

Bottom Line: As such, CXCL12 was retained in the ER.Specific receptor CXCR4 binds to the CXCL12-KDEL, was also retained in the ER, and was thus prevented from reaching the oral squamous cancer cell surface.We reduced the cell surface level of CXCR4 and called the technique "intracellular sequestration." By this way, we have finished blocking of CXCL12-CXCR4 biological axis and inhibiting lymph node metastasis of oral carcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Maxillofacial and ENT, National Cancer Clinical Research Center, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.

ABSTRACT
The CXCL12-CXCR4 biological axis consisting of the chemotactic factor CXCL12 and its specific receptor CXCR4 plays an important role in oral cancer metastasis. High expression of CXCR4 may help oral squamous cancer cells invade local tissues and metastasize to lymph nodes. No obvious association was observed between CXCL12 expression and lymph node metastasis, suggesting that CXCL12 chemotaxis may only be related to CXCR4 expression on the tumor cell membrane. KDEL can be retained by receptors on the surface of the intracellular endoplasmic reticulum (ER) and also be called an ER retention signal sequence. So we adopted the KDEL sequence in this study to generate a CXCL12-KDEL fusion protein in combination with a traceable E-tag label. As such, CXCL12 was retained in the ER. Specific receptor CXCR4 binds to the CXCL12-KDEL, was also retained in the ER, and was thus prevented from reaching the oral squamous cancer cell surface. We reduced the cell surface level of CXCR4 and called the technique "intracellular sequestration." By this way, we have finished blocking of CXCL12-CXCR4 biological axis and inhibiting lymph node metastasis of oral carcinoma.

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CXCL12 and CXCR4 expression levels in neck lymph nodes.
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fig4: CXCL12 and CXCR4 expression levels in neck lymph nodes.

Mentions: Statistical analysis showed that the expression of CXCR4 in lymph nodes with metastatic tumor was significantly higher than that of the nonmetastatic lymph nodes (P < 0.05), and there was no significant difference of CXCL12 expression in lymph nodes between the two groups (P > 0.05) (as shown in Figure 4 and Table 3).


Construction of a CXCL12-KDEL fusion gene to inhibit head and neck squamous cell carcinoma metastasis by intracellular sequestration of CXCR4.

Zhang W, Wang X, Yue K, Liu S, Liu X - Biomed Res Int (2015)

CXCL12 and CXCR4 expression levels in neck lymph nodes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383411&req=5

fig4: CXCL12 and CXCR4 expression levels in neck lymph nodes.
Mentions: Statistical analysis showed that the expression of CXCR4 in lymph nodes with metastatic tumor was significantly higher than that of the nonmetastatic lymph nodes (P < 0.05), and there was no significant difference of CXCL12 expression in lymph nodes between the two groups (P > 0.05) (as shown in Figure 4 and Table 3).

Bottom Line: As such, CXCL12 was retained in the ER.Specific receptor CXCR4 binds to the CXCL12-KDEL, was also retained in the ER, and was thus prevented from reaching the oral squamous cancer cell surface.We reduced the cell surface level of CXCR4 and called the technique "intracellular sequestration." By this way, we have finished blocking of CXCL12-CXCR4 biological axis and inhibiting lymph node metastasis of oral carcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Maxillofacial and ENT, National Cancer Clinical Research Center, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.

ABSTRACT
The CXCL12-CXCR4 biological axis consisting of the chemotactic factor CXCL12 and its specific receptor CXCR4 plays an important role in oral cancer metastasis. High expression of CXCR4 may help oral squamous cancer cells invade local tissues and metastasize to lymph nodes. No obvious association was observed between CXCL12 expression and lymph node metastasis, suggesting that CXCL12 chemotaxis may only be related to CXCR4 expression on the tumor cell membrane. KDEL can be retained by receptors on the surface of the intracellular endoplasmic reticulum (ER) and also be called an ER retention signal sequence. So we adopted the KDEL sequence in this study to generate a CXCL12-KDEL fusion protein in combination with a traceable E-tag label. As such, CXCL12 was retained in the ER. Specific receptor CXCR4 binds to the CXCL12-KDEL, was also retained in the ER, and was thus prevented from reaching the oral squamous cancer cell surface. We reduced the cell surface level of CXCR4 and called the technique "intracellular sequestration." By this way, we have finished blocking of CXCL12-CXCR4 biological axis and inhibiting lymph node metastasis of oral carcinoma.

Show MeSH
Related in: MedlinePlus