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Protecting intestinal epithelial cell number 6 against fission neutron irradiation through NF-κB signaling pathway.

Chang GM, Gao YB, Wang SM, Xu XP, Zhao L, Zhang J, Li JF, Wang YL, Peng RY - Biomed Res Int (2015)

Bottom Line: The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002.Except the control group, the other groups were irradiated by neutron of 4 Gy.At 6 h and 24 h after neutron irradiation of 4 Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China ; Department of Medical Oncology, Air Force PLA General Hospital, Fucheng Road No. 30, Haidian District, Beijing 100142, China.

ABSTRACT
The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002. Three groups of IEC-6 cell lines were given: control group, neutron irradiation of 4 Gy group, and neutron irradiation of 4 Gy with LY294002 treatment group. Except the control group, the other groups were irradiated by neutron of 4 Gy. LY294002 was given before 24 hours of neutron irradiation. At 6 h and 24 h after neutron irradiation, the morphologic changes, proliferation ability, apoptosis, and necrosis rates of the IEC-6 cell lines were assayed and the changes of NF-κB and PI3K/Akt pathway were detected. At 6 h and 24 h after neutron irradiation of 4 Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found. The injuries in LY294002 treatment and neutron irradiation group were more serious than those in control and neutron irradiation groups. The results suggest that IEC-6 cells were obviously damaged and induced serious apoptosis and necrosis by neutron irradiation of 4Gy; the NF-κB signaling pathway in IEC-6 was activated by neutron irradiation which could protect IEC-6 against injury by neutron irradiation; LY294002 could inhibit the activity of IEC-6 cells.

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The expressions of key molecules of NF-κB signaling pathway in IEC-6 cell lines after neutron irradiation of 4Gy and using of LY294002 (C: control group; N: neutron irradiation group; LY: neutron irradiation of 4Gy with LY294002 treatment group).
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fig4: The expressions of key molecules of NF-κB signaling pathway in IEC-6 cell lines after neutron irradiation of 4Gy and using of LY294002 (C: control group; N: neutron irradiation group; LY: neutron irradiation of 4Gy with LY294002 treatment group).

Mentions: The apoptosis and necrosis rates of the IEC-6 cells were increased obviously at 6 h and 24 h after neutron irradiation of 4Gy. The apoptosis rates of the IEC-6 cells were the peak value at 6 h after neutron irradiation, while most of the cells appeared necrosis at 24 h after neutron irradiation. The apoptosis and necrosis rates of the LY294002 treatment group IEC-6 cells were higher than those of the neutron irradiation group cells. Figure 3(a) (A, B, C, D, E, F) showed the scatterplot of apoptosis and necrosis of IEC-6 exposed to neutron irradiation of 4Gy and treated by LY294002. Figures 3(b) and 3(c) showed the results of statistical analysis.


Protecting intestinal epithelial cell number 6 against fission neutron irradiation through NF-κB signaling pathway.

Chang GM, Gao YB, Wang SM, Xu XP, Zhao L, Zhang J, Li JF, Wang YL, Peng RY - Biomed Res Int (2015)

The expressions of key molecules of NF-κB signaling pathway in IEC-6 cell lines after neutron irradiation of 4Gy and using of LY294002 (C: control group; N: neutron irradiation group; LY: neutron irradiation of 4Gy with LY294002 treatment group).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383408&req=5

fig4: The expressions of key molecules of NF-κB signaling pathway in IEC-6 cell lines after neutron irradiation of 4Gy and using of LY294002 (C: control group; N: neutron irradiation group; LY: neutron irradiation of 4Gy with LY294002 treatment group).
Mentions: The apoptosis and necrosis rates of the IEC-6 cells were increased obviously at 6 h and 24 h after neutron irradiation of 4Gy. The apoptosis rates of the IEC-6 cells were the peak value at 6 h after neutron irradiation, while most of the cells appeared necrosis at 24 h after neutron irradiation. The apoptosis and necrosis rates of the LY294002 treatment group IEC-6 cells were higher than those of the neutron irradiation group cells. Figure 3(a) (A, B, C, D, E, F) showed the scatterplot of apoptosis and necrosis of IEC-6 exposed to neutron irradiation of 4Gy and treated by LY294002. Figures 3(b) and 3(c) showed the results of statistical analysis.

Bottom Line: The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002.Except the control group, the other groups were irradiated by neutron of 4 Gy.At 6 h and 24 h after neutron irradiation of 4 Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China ; Department of Medical Oncology, Air Force PLA General Hospital, Fucheng Road No. 30, Haidian District, Beijing 100142, China.

ABSTRACT
The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002. Three groups of IEC-6 cell lines were given: control group, neutron irradiation of 4 Gy group, and neutron irradiation of 4 Gy with LY294002 treatment group. Except the control group, the other groups were irradiated by neutron of 4 Gy. LY294002 was given before 24 hours of neutron irradiation. At 6 h and 24 h after neutron irradiation, the morphologic changes, proliferation ability, apoptosis, and necrosis rates of the IEC-6 cell lines were assayed and the changes of NF-κB and PI3K/Akt pathway were detected. At 6 h and 24 h after neutron irradiation of 4 Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found. The injuries in LY294002 treatment and neutron irradiation group were more serious than those in control and neutron irradiation groups. The results suggest that IEC-6 cells were obviously damaged and induced serious apoptosis and necrosis by neutron irradiation of 4Gy; the NF-κB signaling pathway in IEC-6 was activated by neutron irradiation which could protect IEC-6 against injury by neutron irradiation; LY294002 could inhibit the activity of IEC-6 cells.

Show MeSH
Related in: MedlinePlus