Limits...
Occurrence of West Nile virus antibodies in wild birds, horses, and humans in Poland.

Niczyporuk JS, Samorek-Salamonowicz E, Lecollinet S, Pancewicz SA, Kozdruń W, Czekaj H - Biomed Res Int (2015)

Bottom Line: West Nile virus (WNV) antibodies were detected using competition enzyme linked immunosorbent assays: ELISA-1 ID Screen West Nile Competition, IDvet, ELISA-2 ID Screen West Nile IgM Capture, and ELISA-3 Ingezim West Nile Compac.Fourteen (33.33%) out of 42 sera from patients were positive against WNV antigen and one serum was doubtful.Positive samples obtained in birds were next retested with virus microneutralisation test to confirm positive results and cross-reactions with other antigens of the Japanese encephalitis complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Viral Diseases, National Veterinary Research Institute Pulawy (NVRI), Al. Partyzantow 57, 24-100 Pulawy, Poland.

ABSTRACT
Serum samples of 474 wild birds, 378 horses, and 42 humans with meningitis and lymphocytic meningitis were collected between 2010 and 2014 from different areas of Poland. West Nile virus (WNV) antibodies were detected using competition enzyme linked immunosorbent assays: ELISA-1 ID Screen West Nile Competition, IDvet, ELISA-2 ID Screen West Nile IgM Capture, and ELISA-3 Ingezim West Nile Compac. The antibodies were found in 63 (13.29%) out of 474 wild bird serum samples and in one (0.26%) out of 378 horse serum samples. Fourteen (33.33%) out of 42 sera from patients were positive against WNV antigen and one serum was doubtful. Positive samples obtained in birds were next retested with virus microneutralisation test to confirm positive results and cross-reactions with other antigens of the Japanese encephalitis complex. We suspect that positive serological results in humans, birds, and horses indicate that WNV can be somehow closely related with the ecosystem in Poland.

Show MeSH

Related in: MedlinePlus

Map of Poland. Areas where the samples were collected.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4383358&req=5

fig1: Map of Poland. Areas where the samples were collected.

Mentions: Birds. Serum samples from 474 wild birds, 400 white storks (Ciconia ciconia), 21 common pheasants (Phasianus colchicus), 19 common chaffinches (Fringilla coelebs), nine wild ducks (Anas platyrhynchos), four white-tailed Eagles (Haliaeetus albicilla), two western capercaillies (Tetrao urogallus), six passenger pigeons (Ectopistes migratorius), three northern goshawks (Accipiter gentilis), two hooded crows (Corvus cornix), three northern goshawks (Accipiter gentilis), and one common swift (Apus apus), common blackbird (Turdus merula), common starling (Sturnus vulgaris), common raven (Corvus corax), and common buzzard (Buteo buteo), were collected in different locations in Poland (Zoological Gardens, Rehabilitation Centre of Protected Animals). However, most of the samples derived from Wild Birds Rehabilitation Center, Albatros Foundation. Samples have been collected during March–October, 2010–2014, when the mosquito's activity was the highest (Figure 1).


Occurrence of West Nile virus antibodies in wild birds, horses, and humans in Poland.

Niczyporuk JS, Samorek-Salamonowicz E, Lecollinet S, Pancewicz SA, Kozdruń W, Czekaj H - Biomed Res Int (2015)

Map of Poland. Areas where the samples were collected.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383358&req=5

fig1: Map of Poland. Areas where the samples were collected.
Mentions: Birds. Serum samples from 474 wild birds, 400 white storks (Ciconia ciconia), 21 common pheasants (Phasianus colchicus), 19 common chaffinches (Fringilla coelebs), nine wild ducks (Anas platyrhynchos), four white-tailed Eagles (Haliaeetus albicilla), two western capercaillies (Tetrao urogallus), six passenger pigeons (Ectopistes migratorius), three northern goshawks (Accipiter gentilis), two hooded crows (Corvus cornix), three northern goshawks (Accipiter gentilis), and one common swift (Apus apus), common blackbird (Turdus merula), common starling (Sturnus vulgaris), common raven (Corvus corax), and common buzzard (Buteo buteo), were collected in different locations in Poland (Zoological Gardens, Rehabilitation Centre of Protected Animals). However, most of the samples derived from Wild Birds Rehabilitation Center, Albatros Foundation. Samples have been collected during March–October, 2010–2014, when the mosquito's activity was the highest (Figure 1).

Bottom Line: West Nile virus (WNV) antibodies were detected using competition enzyme linked immunosorbent assays: ELISA-1 ID Screen West Nile Competition, IDvet, ELISA-2 ID Screen West Nile IgM Capture, and ELISA-3 Ingezim West Nile Compac.Fourteen (33.33%) out of 42 sera from patients were positive against WNV antigen and one serum was doubtful.Positive samples obtained in birds were next retested with virus microneutralisation test to confirm positive results and cross-reactions with other antigens of the Japanese encephalitis complex.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Viral Diseases, National Veterinary Research Institute Pulawy (NVRI), Al. Partyzantow 57, 24-100 Pulawy, Poland.

ABSTRACT
Serum samples of 474 wild birds, 378 horses, and 42 humans with meningitis and lymphocytic meningitis were collected between 2010 and 2014 from different areas of Poland. West Nile virus (WNV) antibodies were detected using competition enzyme linked immunosorbent assays: ELISA-1 ID Screen West Nile Competition, IDvet, ELISA-2 ID Screen West Nile IgM Capture, and ELISA-3 Ingezim West Nile Compac. The antibodies were found in 63 (13.29%) out of 474 wild bird serum samples and in one (0.26%) out of 378 horse serum samples. Fourteen (33.33%) out of 42 sera from patients were positive against WNV antigen and one serum was doubtful. Positive samples obtained in birds were next retested with virus microneutralisation test to confirm positive results and cross-reactions with other antigens of the Japanese encephalitis complex. We suspect that positive serological results in humans, birds, and horses indicate that WNV can be somehow closely related with the ecosystem in Poland.

Show MeSH
Related in: MedlinePlus