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A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE.

Pfannkoch EA, Stuff JR, Whitecavage JA, Blevins JM, Seely KA, Moran JH - Int J Anal Chem (2015)

Bottom Line: National Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels of crude oil into the Gulf of Mexico.Stir bar sorptive extraction (SBSE) is an effective technique to extract trace PAHs in water and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction strategy effectively extracts PAHs from complex food matrices.This study uses SBSE to concentrate PAHs and eliminate matrix interference from QuEChERS extracts of seafood, specifically oysters, fish, and shrimp.

View Article: PubMed Central - PubMed

Affiliation: GERSTEL Inc., 701 Digital Drive, Suite J, Linthicum, MD 21090, USA.

ABSTRACT
National Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels of crude oil into the Gulf of Mexico. This method has limited throughput capacity; hence, alternative methods are necessary to meet analytical demands after such events. Stir bar sorptive extraction (SBSE) is an effective technique to extract trace PAHs in water and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction strategy effectively extracts PAHs from complex food matrices. This study uses SBSE to concentrate PAHs and eliminate matrix interference from QuEChERS extracts of seafood, specifically oysters, fish, and shrimp. This method provides acceptable recovery (65-138%) linear calibrations and is sensitive (LOD = 0.02 ppb, LOQ = 0.06 ppb) while providing higher throughput and maintaining equivalency between NOAA 2004 as determined by analysis of NIST SRM 1974b mussel tissue.

No MeSH data available.


Related in: MedlinePlus

Flowchart demonstrating high throughput application of QuEChERS/SBSE method with one technician completing about 60 samples per workday.
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fig3: Flowchart demonstrating high throughput application of QuEChERS/SBSE method with one technician completing about 60 samples per workday.

Mentions: The QuEChERS/SBSE method is inexpensive since the stir bars can be cleaned and reused for 30–50 samples, resulting in an estimated cost of <$10 per sample for all consumables, including QuEChERS kits. In addition, we propose that the QuEChERS/SBSE procedure can provide high sample throughput. The workflow possible by a single analyst is illustrated in Figure 3. The red bars represent the sample processing steps for a single batch of 20 samples prepared by first performing QuEChERS extractions on a batch of 20 samples followed by unattended SBSE for 90 minutes. Samples are loaded onto the GC/MS and an automated analysis is started. It is possible for a single analyst to prepare a second batch of 20 samples (blue bars) and even a third 20-sample batch (green bars) per day which can be added to the automated GC/MS analysis. The length of the GC/MS method, or other analysis, may vary, but results can be obtained for about 60 samples per day.


A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE.

Pfannkoch EA, Stuff JR, Whitecavage JA, Blevins JM, Seely KA, Moran JH - Int J Anal Chem (2015)

Flowchart demonstrating high throughput application of QuEChERS/SBSE method with one technician completing about 60 samples per workday.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383357&req=5

fig3: Flowchart demonstrating high throughput application of QuEChERS/SBSE method with one technician completing about 60 samples per workday.
Mentions: The QuEChERS/SBSE method is inexpensive since the stir bars can be cleaned and reused for 30–50 samples, resulting in an estimated cost of <$10 per sample for all consumables, including QuEChERS kits. In addition, we propose that the QuEChERS/SBSE procedure can provide high sample throughput. The workflow possible by a single analyst is illustrated in Figure 3. The red bars represent the sample processing steps for a single batch of 20 samples prepared by first performing QuEChERS extractions on a batch of 20 samples followed by unattended SBSE for 90 minutes. Samples are loaded onto the GC/MS and an automated analysis is started. It is possible for a single analyst to prepare a second batch of 20 samples (blue bars) and even a third 20-sample batch (green bars) per day which can be added to the automated GC/MS analysis. The length of the GC/MS method, or other analysis, may vary, but results can be obtained for about 60 samples per day.

Bottom Line: National Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels of crude oil into the Gulf of Mexico.Stir bar sorptive extraction (SBSE) is an effective technique to extract trace PAHs in water and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction strategy effectively extracts PAHs from complex food matrices.This study uses SBSE to concentrate PAHs and eliminate matrix interference from QuEChERS extracts of seafood, specifically oysters, fish, and shrimp.

View Article: PubMed Central - PubMed

Affiliation: GERSTEL Inc., 701 Digital Drive, Suite J, Linthicum, MD 21090, USA.

ABSTRACT
National Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels of crude oil into the Gulf of Mexico. This method has limited throughput capacity; hence, alternative methods are necessary to meet analytical demands after such events. Stir bar sorptive extraction (SBSE) is an effective technique to extract trace PAHs in water and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction strategy effectively extracts PAHs from complex food matrices. This study uses SBSE to concentrate PAHs and eliminate matrix interference from QuEChERS extracts of seafood, specifically oysters, fish, and shrimp. This method provides acceptable recovery (65-138%) linear calibrations and is sensitive (LOD = 0.02 ppb, LOQ = 0.06 ppb) while providing higher throughput and maintaining equivalency between NOAA 2004 as determined by analysis of NIST SRM 1974b mussel tissue.

No MeSH data available.


Related in: MedlinePlus