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The expression and distributions of ANP32A in the developing brain.

Wang S, Wang Y, Lu Q, Liu X, Wang F, Ma X, Cui C, Shi C, Li J, Zhang D - Biomed Res Int (2015)

Bottom Line: However, whether ANP32A has an effect on the mammalian developing brain is still in question.The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord.The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

View Article: PubMed Central - PubMed

Affiliation: Weifang Medical University, Weifang 261042, China.

ABSTRACT
Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A), has multiple functions involved in neuritogenesis, transcriptional regulation, and apoptosis. However, whether ANP32A has an effect on the mammalian developing brain is still in question. In this study, it was shown that brain was the organ that expressed the most abundant ANP32A by human multiple tissue expression (MTE) array. The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord. The expression of ANP32A was higher in the adult brain than in the fetal brain of not only humans but also mice in a time-dependent manner. ANP32A signals were dispersed accordantly in embryonic mouse brain. However, ANP32A was abundant in the granular layer of the cerebellum and the cerebral cortex when the mice were growing up, as well as in the Purkinje cells of the cerebellum. The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

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Immunohistochemical study of ANP32A in mouse cerebral cortex. ANP32A signal was visualized with DAB in C57 BL/6 brain. E12 and E16, embryonic days 12 and 16, respectively; P0, P5, and P12, postnatal days 0, 5, and 12, respectively; 5-6 W, adult brain from 5-6 weeks old C57 BL/6. Scale bar = 200 μm. Granule cells were marked by red arrow.
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fig3: Immunohistochemical study of ANP32A in mouse cerebral cortex. ANP32A signal was visualized with DAB in C57 BL/6 brain. E12 and E16, embryonic days 12 and 16, respectively; P0, P5, and P12, postnatal days 0, 5, and 12, respectively; 5-6 W, adult brain from 5-6 weeks old C57 BL/6. Scale bar = 200 μm. Granule cells were marked by red arrow.

Mentions: Compared with the embryonic mouse, ANP32A protein was expressed along with the differential cerebral cortex's layer, more and more significantly, after birth. The changes of morphology of the positive stained cells were also coincidental to the development of the neuron in the nervous system. In the embryonic period, the layer of cerebral cortex is not clear. All positive staining cells are small and numerous, and the expression of ANP32A seemed to be nothing special. When the mice were birthed and growing, the positive staining cells were bigger and more strongly stained in external granular layer of the cerebral cortex (Figure 3).


The expression and distributions of ANP32A in the developing brain.

Wang S, Wang Y, Lu Q, Liu X, Wang F, Ma X, Cui C, Shi C, Li J, Zhang D - Biomed Res Int (2015)

Immunohistochemical study of ANP32A in mouse cerebral cortex. ANP32A signal was visualized with DAB in C57 BL/6 brain. E12 and E16, embryonic days 12 and 16, respectively; P0, P5, and P12, postnatal days 0, 5, and 12, respectively; 5-6 W, adult brain from 5-6 weeks old C57 BL/6. Scale bar = 200 μm. Granule cells were marked by red arrow.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383345&req=5

fig3: Immunohistochemical study of ANP32A in mouse cerebral cortex. ANP32A signal was visualized with DAB in C57 BL/6 brain. E12 and E16, embryonic days 12 and 16, respectively; P0, P5, and P12, postnatal days 0, 5, and 12, respectively; 5-6 W, adult brain from 5-6 weeks old C57 BL/6. Scale bar = 200 μm. Granule cells were marked by red arrow.
Mentions: Compared with the embryonic mouse, ANP32A protein was expressed along with the differential cerebral cortex's layer, more and more significantly, after birth. The changes of morphology of the positive stained cells were also coincidental to the development of the neuron in the nervous system. In the embryonic period, the layer of cerebral cortex is not clear. All positive staining cells are small and numerous, and the expression of ANP32A seemed to be nothing special. When the mice were birthed and growing, the positive staining cells were bigger and more strongly stained in external granular layer of the cerebral cortex (Figure 3).

Bottom Line: However, whether ANP32A has an effect on the mammalian developing brain is still in question.The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord.The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

View Article: PubMed Central - PubMed

Affiliation: Weifang Medical University, Weifang 261042, China.

ABSTRACT
Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A), has multiple functions involved in neuritogenesis, transcriptional regulation, and apoptosis. However, whether ANP32A has an effect on the mammalian developing brain is still in question. In this study, it was shown that brain was the organ that expressed the most abundant ANP32A by human multiple tissue expression (MTE) array. The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord. The expression of ANP32A was higher in the adult brain than in the fetal brain of not only humans but also mice in a time-dependent manner. ANP32A signals were dispersed accordantly in embryonic mouse brain. However, ANP32A was abundant in the granular layer of the cerebellum and the cerebral cortex when the mice were growing up, as well as in the Purkinje cells of the cerebellum. The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

Show MeSH