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The expression and distributions of ANP32A in the developing brain.

Wang S, Wang Y, Lu Q, Liu X, Wang F, Ma X, Cui C, Shi C, Li J, Zhang D - Biomed Res Int (2015)

Bottom Line: However, whether ANP32A has an effect on the mammalian developing brain is still in question.The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord.The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

View Article: PubMed Central - PubMed

Affiliation: Weifang Medical University, Weifang 261042, China.

ABSTRACT
Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A), has multiple functions involved in neuritogenesis, transcriptional regulation, and apoptosis. However, whether ANP32A has an effect on the mammalian developing brain is still in question. In this study, it was shown that brain was the organ that expressed the most abundant ANP32A by human multiple tissue expression (MTE) array. The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord. The expression of ANP32A was higher in the adult brain than in the fetal brain of not only humans but also mice in a time-dependent manner. ANP32A signals were dispersed accordantly in embryonic mouse brain. However, ANP32A was abundant in the granular layer of the cerebellum and the cerebral cortex when the mice were growing up, as well as in the Purkinje cells of the cerebellum. The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

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Distribution of human ANP32A transcripts in brain. Human MTE array was probed with a 750 bp human ANP32A radiolabeled probe as described under “experimental procedures.” mRNA levels were determined by densitometric scanning of autoradiographs. Whole brain and fetal brain are shown as red column, and different anatomical region is shown as blue column.
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fig1: Distribution of human ANP32A transcripts in brain. Human MTE array was probed with a 750 bp human ANP32A radiolabeled probe as described under “experimental procedures.” mRNA levels were determined by densitometric scanning of autoradiographs. Whole brain and fetal brain are shown as red column, and different anatomical region is shown as blue column.

Mentions: The differences of the levels of ANP32A mRNA between various areas of human brain were tested by the spot hybridized with the human MTE assay. The MTE array provides a fast way to simultaneously compare the relative abundance of ANP32A mRNA in a wide array of tissues, normalized and immobilized in separate dots, along with several controls. It was shown that brain was the organ that expressed the most abundant ANP32A, followed with heart, liver, and kidney. As shown with blue column in Figure 1, the expression levels of ANP32A mRNA were fluctuated in different areas of human brain. Among 20 different brain tissues, cerebellum right is the area with most abundant ANP32A; next is temporal lobe followed with cerebellum left, nucleus accumbens, substantia nigra, and cerebral cortex. Although ANP32A was abundant in the most brain areas, it was hardly detected in the pons. ANP32A could be slightly detected in medulla oblongata and spinal cord, a little higher than amygdala and parietal lobe.


The expression and distributions of ANP32A in the developing brain.

Wang S, Wang Y, Lu Q, Liu X, Wang F, Ma X, Cui C, Shi C, Li J, Zhang D - Biomed Res Int (2015)

Distribution of human ANP32A transcripts in brain. Human MTE array was probed with a 750 bp human ANP32A radiolabeled probe as described under “experimental procedures.” mRNA levels were determined by densitometric scanning of autoradiographs. Whole brain and fetal brain are shown as red column, and different anatomical region is shown as blue column.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383345&req=5

fig1: Distribution of human ANP32A transcripts in brain. Human MTE array was probed with a 750 bp human ANP32A radiolabeled probe as described under “experimental procedures.” mRNA levels were determined by densitometric scanning of autoradiographs. Whole brain and fetal brain are shown as red column, and different anatomical region is shown as blue column.
Mentions: The differences of the levels of ANP32A mRNA between various areas of human brain were tested by the spot hybridized with the human MTE assay. The MTE array provides a fast way to simultaneously compare the relative abundance of ANP32A mRNA in a wide array of tissues, normalized and immobilized in separate dots, along with several controls. It was shown that brain was the organ that expressed the most abundant ANP32A, followed with heart, liver, and kidney. As shown with blue column in Figure 1, the expression levels of ANP32A mRNA were fluctuated in different areas of human brain. Among 20 different brain tissues, cerebellum right is the area with most abundant ANP32A; next is temporal lobe followed with cerebellum left, nucleus accumbens, substantia nigra, and cerebral cortex. Although ANP32A was abundant in the most brain areas, it was hardly detected in the pons. ANP32A could be slightly detected in medulla oblongata and spinal cord, a little higher than amygdala and parietal lobe.

Bottom Line: However, whether ANP32A has an effect on the mammalian developing brain is still in question.The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord.The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

View Article: PubMed Central - PubMed

Affiliation: Weifang Medical University, Weifang 261042, China.

ABSTRACT
Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A), has multiple functions involved in neuritogenesis, transcriptional regulation, and apoptosis. However, whether ANP32A has an effect on the mammalian developing brain is still in question. In this study, it was shown that brain was the organ that expressed the most abundant ANP32A by human multiple tissue expression (MTE) array. The distribution of ANP32A in the different adult brain areas was diverse dramatically, with high expression in cerebellum, temporal lobe, and cerebral cortex and with low expression in pons, medulla oblongata, and spinal cord. The expression of ANP32A was higher in the adult brain than in the fetal brain of not only humans but also mice in a time-dependent manner. ANP32A signals were dispersed accordantly in embryonic mouse brain. However, ANP32A was abundant in the granular layer of the cerebellum and the cerebral cortex when the mice were growing up, as well as in the Purkinje cells of the cerebellum. The variation of expression levels and distribution of ANP32A in the developing brain would imply that ANP32A may play an important role in mammalian brain development, especially in the differentiation and function of neurons in the cerebellum and the cerebral cortex.

Show MeSH