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Post-mortem interval estimation of human skeletal remains by micro-computed tomography, mid-infrared microscopic imaging and energy dispersive X-ray mapping.

Longato S, Wöss C, Hatzer-Grubwieser P, Bauer C, Parson W, Unterberger SH, Kuhn V, Pemberger N, Pallua AK, Recheis W, Lackner R, Stalder R, Pallua JD - Anal Methods (2015)

Bottom Line: In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved.It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation.Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

View Article: PubMed Central - PubMed

Affiliation: Institute of Legal Medicine , Medical University of Innsbruck , Müllerstraße 44 , 6020 Innsbruck , Austria . Email: Johannes.Pallua@i-med.ac.at.

ABSTRACT

In this study different state-of-the-art visualization methods such as micro-computed tomography (micro-CT), mid-infrared (MIR) microscopic imaging and energy dispersive X-ray (EDS) mapping were evaluated to study human skeletal remains for the determination of the post-mortem interval (PMI). PMI specific features were identified and visualized by overlaying molecular imaging data and morphological tissue structures generated by radiological techniques and microscopic images gained from confocal microscopy (Infinite Focus (IFM)). In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved. It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation. Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

No MeSH data available.


Related in: MedlinePlus

(A) IFM micrographs of individual patient's tissue samples. (B and C) Infrared spectroscopic maps of the bone surface obtained for the fluoridation at 605 cm–1 and bone mineral at 1042 cm–1.
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fig4: (A) IFM micrographs of individual patient's tissue samples. (B and C) Infrared spectroscopic maps of the bone surface obtained for the fluoridation at 605 cm–1 and bone mineral at 1042 cm–1.

Mentions: Analyses of the resulting MIR microscopic imaging datasets were performed using the mentioned software packages. In this study, 6 bone samples with different PMIs were analysed by individual chemi-map representations, spectra-analysis and principle component analyses (PCA) (Fig. 4 and 5).


Post-mortem interval estimation of human skeletal remains by micro-computed tomography, mid-infrared microscopic imaging and energy dispersive X-ray mapping.

Longato S, Wöss C, Hatzer-Grubwieser P, Bauer C, Parson W, Unterberger SH, Kuhn V, Pemberger N, Pallua AK, Recheis W, Lackner R, Stalder R, Pallua JD - Anal Methods (2015)

(A) IFM micrographs of individual patient's tissue samples. (B and C) Infrared spectroscopic maps of the bone surface obtained for the fluoridation at 605 cm–1 and bone mineral at 1042 cm–1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383336&req=5

fig4: (A) IFM micrographs of individual patient's tissue samples. (B and C) Infrared spectroscopic maps of the bone surface obtained for the fluoridation at 605 cm–1 and bone mineral at 1042 cm–1.
Mentions: Analyses of the resulting MIR microscopic imaging datasets were performed using the mentioned software packages. In this study, 6 bone samples with different PMIs were analysed by individual chemi-map representations, spectra-analysis and principle component analyses (PCA) (Fig. 4 and 5).

Bottom Line: In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved.It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation.Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

View Article: PubMed Central - PubMed

Affiliation: Institute of Legal Medicine , Medical University of Innsbruck , Müllerstraße 44 , 6020 Innsbruck , Austria . Email: Johannes.Pallua@i-med.ac.at.

ABSTRACT

In this study different state-of-the-art visualization methods such as micro-computed tomography (micro-CT), mid-infrared (MIR) microscopic imaging and energy dispersive X-ray (EDS) mapping were evaluated to study human skeletal remains for the determination of the post-mortem interval (PMI). PMI specific features were identified and visualized by overlaying molecular imaging data and morphological tissue structures generated by radiological techniques and microscopic images gained from confocal microscopy (Infinite Focus (IFM)). In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved. It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation. Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

No MeSH data available.


Related in: MedlinePlus