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Post-mortem interval estimation of human skeletal remains by micro-computed tomography, mid-infrared microscopic imaging and energy dispersive X-ray mapping.

Longato S, Wöss C, Hatzer-Grubwieser P, Bauer C, Parson W, Unterberger SH, Kuhn V, Pemberger N, Pallua AK, Recheis W, Lackner R, Stalder R, Pallua JD - Anal Methods (2015)

Bottom Line: In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved.It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation.Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

View Article: PubMed Central - PubMed

Affiliation: Institute of Legal Medicine , Medical University of Innsbruck , Müllerstraße 44 , 6020 Innsbruck , Austria . Email: Johannes.Pallua@i-med.ac.at.

ABSTRACT

In this study different state-of-the-art visualization methods such as micro-computed tomography (micro-CT), mid-infrared (MIR) microscopic imaging and energy dispersive X-ray (EDS) mapping were evaluated to study human skeletal remains for the determination of the post-mortem interval (PMI). PMI specific features were identified and visualized by overlaying molecular imaging data and morphological tissue structures generated by radiological techniques and microscopic images gained from confocal microscopy (Infinite Focus (IFM)). In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved. It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation. Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

No MeSH data available.


IR spectra from a bone sample, displayed in the range of 4000 cm–1 to 850 cm–1. Major absorption bands are indicated. Spectra demonstrate a slight increase in noise as the aperture size decreases. The C–H stretching vibrations between 2956 cm–1 and 2800 cm–1 can only be recorded by mapping with an aperture size of 140 μm × 140 μm. The absorption feature between 2300 and 2400 cm–1 is due to atmospheric CO2 and does not interfere with bands relevant in this study.
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fig3: IR spectra from a bone sample, displayed in the range of 4000 cm–1 to 850 cm–1. Major absorption bands are indicated. Spectra demonstrate a slight increase in noise as the aperture size decreases. The C–H stretching vibrations between 2956 cm–1 and 2800 cm–1 can only be recorded by mapping with an aperture size of 140 μm × 140 μm. The absorption feature between 2300 and 2400 cm–1 is due to atmospheric CO2 and does not interfere with bands relevant in this study.

Mentions: To illustrate the effects of the beam focus on spectra quality, representative spectra chosen from the same region of one individual bone sample recorded by the two methods are compared (Fig. 3). It can be demonstrated, that there is no detrimental effect of the measurement settings on the spectra quality in the range of 1800 cm–1 to 850 cm–1. The C–H stretching vibrations of phospholipids, protein side chains as well as nucleic acid sugars between 2956 cm–1 and 2800 cm–1 can only be recorded by mapping with an aperture size of 140 μm × 140 μm. Therefore, further mapping measurements were performed with an aperture size of 140 μm × 140 μm. With this measurement mode it was possible (a) to record the C–H stretching vibrations between 2956 cm–1 and 2800 cm–1, (b) to reduce the scan time, (c) to collect spectra in the detector range of 4000 cm–1 to 550 cm–1 and (d) to measure large area of the sample, which simplified a correlation with micro-CT results.


Post-mortem interval estimation of human skeletal remains by micro-computed tomography, mid-infrared microscopic imaging and energy dispersive X-ray mapping.

Longato S, Wöss C, Hatzer-Grubwieser P, Bauer C, Parson W, Unterberger SH, Kuhn V, Pemberger N, Pallua AK, Recheis W, Lackner R, Stalder R, Pallua JD - Anal Methods (2015)

IR spectra from a bone sample, displayed in the range of 4000 cm–1 to 850 cm–1. Major absorption bands are indicated. Spectra demonstrate a slight increase in noise as the aperture size decreases. The C–H stretching vibrations between 2956 cm–1 and 2800 cm–1 can only be recorded by mapping with an aperture size of 140 μm × 140 μm. The absorption feature between 2300 and 2400 cm–1 is due to atmospheric CO2 and does not interfere with bands relevant in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383336&req=5

fig3: IR spectra from a bone sample, displayed in the range of 4000 cm–1 to 850 cm–1. Major absorption bands are indicated. Spectra demonstrate a slight increase in noise as the aperture size decreases. The C–H stretching vibrations between 2956 cm–1 and 2800 cm–1 can only be recorded by mapping with an aperture size of 140 μm × 140 μm. The absorption feature between 2300 and 2400 cm–1 is due to atmospheric CO2 and does not interfere with bands relevant in this study.
Mentions: To illustrate the effects of the beam focus on spectra quality, representative spectra chosen from the same region of one individual bone sample recorded by the two methods are compared (Fig. 3). It can be demonstrated, that there is no detrimental effect of the measurement settings on the spectra quality in the range of 1800 cm–1 to 850 cm–1. The C–H stretching vibrations of phospholipids, protein side chains as well as nucleic acid sugars between 2956 cm–1 and 2800 cm–1 can only be recorded by mapping with an aperture size of 140 μm × 140 μm. Therefore, further mapping measurements were performed with an aperture size of 140 μm × 140 μm. With this measurement mode it was possible (a) to record the C–H stretching vibrations between 2956 cm–1 and 2800 cm–1, (b) to reduce the scan time, (c) to collect spectra in the detector range of 4000 cm–1 to 550 cm–1 and (d) to measure large area of the sample, which simplified a correlation with micro-CT results.

Bottom Line: In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved.It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation.Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

View Article: PubMed Central - PubMed

Affiliation: Institute of Legal Medicine , Medical University of Innsbruck , Müllerstraße 44 , 6020 Innsbruck , Austria . Email: Johannes.Pallua@i-med.ac.at.

ABSTRACT

In this study different state-of-the-art visualization methods such as micro-computed tomography (micro-CT), mid-infrared (MIR) microscopic imaging and energy dispersive X-ray (EDS) mapping were evaluated to study human skeletal remains for the determination of the post-mortem interval (PMI). PMI specific features were identified and visualized by overlaying molecular imaging data and morphological tissue structures generated by radiological techniques and microscopic images gained from confocal microscopy (Infinite Focus (IFM)). In this way, a more distinct picture concerning processes during the PMI as well as a more realistic approximation of the PMI were achieved. It could be demonstrated that the gained result in combination with multivariate data analysis can be used to predict the Ca/C ratio and bone volume (BV) over total volume (TV) for PMI estimation. Statistical limitation of this study is the small sample size, and future work will be based on more specimens to develop a screening tool for PMI based on the outcome of this multidimensional approach.

No MeSH data available.