Limits...
Pregabalin and tranexamic Acid evaluation by two simple and sensitive spectrophotometric methods.

Sher N, Fatima N, Perveen S, Siddiqui FA, Wafa Sial A - Int J Anal Chem (2015)

Bottom Line: Both drugs contain the amino group, and when they are reacted with 2,4-dinitrophenol and 2,4,6-trinitrophenol, they give rise to yellow colored complexes showing absorption maximum at 418 nm and 425 nm, respectively, based on the Lewis acid base reaction.Limit of detection was in range from 0.0041 to 0.0094 µgmL(-1) and limit of quantification was in the range from 0.0137 to 0.0302 µgmL(-1).Excellent recovery in Placebo spiked samples indicated that there is no interference from common excipients.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, University of Karachi, Karachi 75270, Pakistan.

ABSTRACT
This paper demonstrates colorimetric visible spectrophotometric quantification methods for amino acid, namely, tranexamic acid and pregabalin. Both drugs contain the amino group, and when they are reacted with 2,4-dinitrophenol and 2,4,6-trinitrophenol, they give rise to yellow colored complexes showing absorption maximum at 418 nm and 425 nm, respectively, based on the Lewis acid base reaction. Detailed optimization process and stoichiometric studies were conducted along with investigation of thermodynamic features, that is, association constant and standard free energy changes. The method was linear over the concentration range of 0.02-200 µgmL(-1) with correlation coefficient of more than 0.9990 in all of the cases. Limit of detection was in range from 0.0041 to 0.0094 µgmL(-1) and limit of quantification was in the range from 0.0137 to 0.0302 µgmL(-1). Excellent recovery in Placebo spiked samples indicated that there is no interference from common excipients. The analytical methods under proposal were successfully applied to determine tranexamic acid and pregabalin in commercial products. t-test and F ratio were evaluated without noticeable difference between the proposed and reference methods.

No MeSH data available.


Structure of PG and TXA.
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fig1: Structure of PG and TXA.

Mentions: Pregabalin (PG) chemically is 3-amino methyl hexanoic acid, with the chemical formula C8H17NO2. Its structural and pharmacological features correspond to the mammalian neurotransmitter gamma-aminobutyric acid (GABA), and it is primarily used as anticonvulsant drug. However, its effects are much broader being analgesic, antiepileptic, antidiabetic, and anti-inflammatory drug. Further more, it has been recommended for gastrointestinal damage, alcoholism, and insomnia [1]. Exact mechanism is not known; however, it has been an established fact that it binds to calcium channel in the central nervous system which decreases calcium influx at nerve endings and therefore reduces the liberation of several associated neurotransmitters which subsequently results in the above-mentioned activities [2]. Tranexamic acid (TXA) is chemically designed as trans-4-(aminomethyl) cyclohexanecarboxylic acid, with chemical formula C8H14NO2. It is the closed cyclic analogous structure of lysine. TXA has been very potent antifibrinolytic agent, vastly used in haemorrhagic diseases. Its significant use is to treat ovarian tumors and it is recommended to manage pregnancy and reduce blood lose in surgery. It is considered as the best substitute to surgery in cases of menorrhagia [3, 4]. Chemical structure of TXA and PG is shown in Figure 1.


Pregabalin and tranexamic Acid evaluation by two simple and sensitive spectrophotometric methods.

Sher N, Fatima N, Perveen S, Siddiqui FA, Wafa Sial A - Int J Anal Chem (2015)

Structure of PG and TXA.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383309&req=5

fig1: Structure of PG and TXA.
Mentions: Pregabalin (PG) chemically is 3-amino methyl hexanoic acid, with the chemical formula C8H17NO2. Its structural and pharmacological features correspond to the mammalian neurotransmitter gamma-aminobutyric acid (GABA), and it is primarily used as anticonvulsant drug. However, its effects are much broader being analgesic, antiepileptic, antidiabetic, and anti-inflammatory drug. Further more, it has been recommended for gastrointestinal damage, alcoholism, and insomnia [1]. Exact mechanism is not known; however, it has been an established fact that it binds to calcium channel in the central nervous system which decreases calcium influx at nerve endings and therefore reduces the liberation of several associated neurotransmitters which subsequently results in the above-mentioned activities [2]. Tranexamic acid (TXA) is chemically designed as trans-4-(aminomethyl) cyclohexanecarboxylic acid, with chemical formula C8H14NO2. It is the closed cyclic analogous structure of lysine. TXA has been very potent antifibrinolytic agent, vastly used in haemorrhagic diseases. Its significant use is to treat ovarian tumors and it is recommended to manage pregnancy and reduce blood lose in surgery. It is considered as the best substitute to surgery in cases of menorrhagia [3, 4]. Chemical structure of TXA and PG is shown in Figure 1.

Bottom Line: Both drugs contain the amino group, and when they are reacted with 2,4-dinitrophenol and 2,4,6-trinitrophenol, they give rise to yellow colored complexes showing absorption maximum at 418 nm and 425 nm, respectively, based on the Lewis acid base reaction.Limit of detection was in range from 0.0041 to 0.0094 µgmL(-1) and limit of quantification was in the range from 0.0137 to 0.0302 µgmL(-1).Excellent recovery in Placebo spiked samples indicated that there is no interference from common excipients.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Faculty of Science, University of Karachi, Karachi 75270, Pakistan.

ABSTRACT
This paper demonstrates colorimetric visible spectrophotometric quantification methods for amino acid, namely, tranexamic acid and pregabalin. Both drugs contain the amino group, and when they are reacted with 2,4-dinitrophenol and 2,4,6-trinitrophenol, they give rise to yellow colored complexes showing absorption maximum at 418 nm and 425 nm, respectively, based on the Lewis acid base reaction. Detailed optimization process and stoichiometric studies were conducted along with investigation of thermodynamic features, that is, association constant and standard free energy changes. The method was linear over the concentration range of 0.02-200 µgmL(-1) with correlation coefficient of more than 0.9990 in all of the cases. Limit of detection was in range from 0.0041 to 0.0094 µgmL(-1) and limit of quantification was in the range from 0.0137 to 0.0302 µgmL(-1). Excellent recovery in Placebo spiked samples indicated that there is no interference from common excipients. The analytical methods under proposal were successfully applied to determine tranexamic acid and pregabalin in commercial products. t-test and F ratio were evaluated without noticeable difference between the proposed and reference methods.

No MeSH data available.