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Identification of the alternative splicing of the UL49 locus of human cytomegalovirus.

Yang G, Li W, Liao W, Zhang X, Zou Y, Dai J, Li Y, Jing C, Zhou T - Biomed Res Int (2015)

Bottom Line: All the new EST sequences and UL49Y cDNA sequence have been deposited in the GenBank database (GenBank Accession nos.GW314860-GW314900 and GU376796).This study provides us with very important clues for revealing the importance of the UL49 locus alternative splicing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China ; Department of Parasitology, Medical School, Jinan University, Guangzhou 510632, China.

ABSTRACT
The UL49 ORF of human cytomegalovirus (HCMV) is essential for viral replication; conserved among all herpes viruses; however, the function is unclear. Once the UL49 ORF was precisely deleted from the start to stop codon, the mutant did not yield infectious progeny. In this study, we find out many alternatively processed ESTs in UL49 locus in HCMV-infected cells, in which there are two novel transcription termination sites in UL49 locus. Most of these ESTs are rare transcripts that contain directed repeat sequences in the intron splicing regions. There is a typical GU-AG intron splicing site in UL49Y transcripts. The 1847 bp UL49Y cDNA spans an ORF from 335 to 1618 and encodes a putative protein of 427 amino acids with a predicted molecular mass of 47.1 kDa. All the new EST sequences and UL49Y cDNA sequence have been deposited in the GenBank database (GenBank Accession nos. GW314860-GW314900 and GU376796). This study provides us with very important clues for revealing the importance of the UL49 locus alternative splicing.

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Related in: MedlinePlus

Nucleotide and deduced amino acid sequence of the UL49Y gene. Numbers on the left refer to the first nucleotide in each corresponding line. An upstream inframe stop codon (TGA) at 5′ to the start codon is shaded in gray. An asterisk indicates the stop codon. The DNA sequence has been deposited in the GenBank database (GenBank Accession no. GU376796).
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fig3: Nucleotide and deduced amino acid sequence of the UL49Y gene. Numbers on the left refer to the first nucleotide in each corresponding line. An upstream inframe stop codon (TGA) at 5′ to the start codon is shaded in gray. An asterisk indicates the stop codon. The DNA sequence has been deposited in the GenBank database (GenBank Accession no. GU376796).

Mentions: The 1847 bp UL49Y cDNA spanned an ORF from 335 to 1618 and encoded a putative protein of 427 amino acids with a predicted molecular mass of 47.1 kDa and an isoelectric point (pI) 9.35. There is an inframe stop codon at −72 prior to the first initiation codon. UL49Y cDNA contained a 95 bp intron and the intron conformed to the GU-AG rule (GGCTGgtgtg…tacagCATGGA). UL49Y is the truncated form of UL49, which lacks 143 aa in the 5′ end of UL49 (Figure 3).


Identification of the alternative splicing of the UL49 locus of human cytomegalovirus.

Yang G, Li W, Liao W, Zhang X, Zou Y, Dai J, Li Y, Jing C, Zhou T - Biomed Res Int (2015)

Nucleotide and deduced amino acid sequence of the UL49Y gene. Numbers on the left refer to the first nucleotide in each corresponding line. An upstream inframe stop codon (TGA) at 5′ to the start codon is shaded in gray. An asterisk indicates the stop codon. The DNA sequence has been deposited in the GenBank database (GenBank Accession no. GU376796).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383306&req=5

fig3: Nucleotide and deduced amino acid sequence of the UL49Y gene. Numbers on the left refer to the first nucleotide in each corresponding line. An upstream inframe stop codon (TGA) at 5′ to the start codon is shaded in gray. An asterisk indicates the stop codon. The DNA sequence has been deposited in the GenBank database (GenBank Accession no. GU376796).
Mentions: The 1847 bp UL49Y cDNA spanned an ORF from 335 to 1618 and encoded a putative protein of 427 amino acids with a predicted molecular mass of 47.1 kDa and an isoelectric point (pI) 9.35. There is an inframe stop codon at −72 prior to the first initiation codon. UL49Y cDNA contained a 95 bp intron and the intron conformed to the GU-AG rule (GGCTGgtgtg…tacagCATGGA). UL49Y is the truncated form of UL49, which lacks 143 aa in the 5′ end of UL49 (Figure 3).

Bottom Line: All the new EST sequences and UL49Y cDNA sequence have been deposited in the GenBank database (GenBank Accession nos.GW314860-GW314900 and GU376796).This study provides us with very important clues for revealing the importance of the UL49 locus alternative splicing.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China ; Department of Parasitology, Medical School, Jinan University, Guangzhou 510632, China.

ABSTRACT
The UL49 ORF of human cytomegalovirus (HCMV) is essential for viral replication; conserved among all herpes viruses; however, the function is unclear. Once the UL49 ORF was precisely deleted from the start to stop codon, the mutant did not yield infectious progeny. In this study, we find out many alternatively processed ESTs in UL49 locus in HCMV-infected cells, in which there are two novel transcription termination sites in UL49 locus. Most of these ESTs are rare transcripts that contain directed repeat sequences in the intron splicing regions. There is a typical GU-AG intron splicing site in UL49Y transcripts. The 1847 bp UL49Y cDNA spans an ORF from 335 to 1618 and encodes a putative protein of 427 amino acids with a predicted molecular mass of 47.1 kDa. All the new EST sequences and UL49Y cDNA sequence have been deposited in the GenBank database (GenBank Accession nos. GW314860-GW314900 and GU376796). This study provides us with very important clues for revealing the importance of the UL49 locus alternative splicing.

Show MeSH
Related in: MedlinePlus