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Application of an activated carbon-based support for magnetic solid phase extraction followed by spectrophotometric determination of tartrazine in commercial beverages.

Rodríguez JA, Escamilla-Lara KA, Guevara-Lara A, Miranda JM, Páez-Hernández ME - Int J Anal Chem (2015)

Bottom Line: The tartrazine eluted from the magnetic support was determined by spectrophotometry.Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1).A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Laboratorio 2, Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, Mexico.

ABSTRACT
A method is presented for magnetic solid phase extraction of tartrazine from nonalcoholic beverages. The method involves the extraction and clean-up by activated carbon covered with magnetite dispersed in the sample, followed by the magnetic isolation and desorption of the analyte by basified methanol. The tartrazine eluted from the magnetic support was determined by spectrophotometry. Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1). The method was validated by comparing the results with those obtained by HPLC. A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

No MeSH data available.


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Chromatograms of (a) original sample and (b) eluted tartrazine solution after using MSPE developed method.
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fig6: Chromatograms of (a) original sample and (b) eluted tartrazine solution after using MSPE developed method.

Mentions: Following the developed and optimized method, the tartrazine concentration for each beverage is shown in Table 7. This value represents the average of three independent determinations. Additionally, in Table 7 it is possible to observe the results for the analysis of samples using the HPLC reference method and MSPE-HPLC (Figure 6). For each beverage, the average concentration of tartrazine obtained using both methods was compared using a t-test with 2 degrees of freedom and 95% of confidence (ttab = 4.3). This analysis revealed no significant differences between the results from each method. Therefore, the methodology of MSPE is comparable with the reference methodology. Additionally, the MSPE is a robust preconcentration technique that can be coupled even to spectrophotometry or HPLC.


Application of an activated carbon-based support for magnetic solid phase extraction followed by spectrophotometric determination of tartrazine in commercial beverages.

Rodríguez JA, Escamilla-Lara KA, Guevara-Lara A, Miranda JM, Páez-Hernández ME - Int J Anal Chem (2015)

Chromatograms of (a) original sample and (b) eluted tartrazine solution after using MSPE developed method.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383302&req=5

fig6: Chromatograms of (a) original sample and (b) eluted tartrazine solution after using MSPE developed method.
Mentions: Following the developed and optimized method, the tartrazine concentration for each beverage is shown in Table 7. This value represents the average of three independent determinations. Additionally, in Table 7 it is possible to observe the results for the analysis of samples using the HPLC reference method and MSPE-HPLC (Figure 6). For each beverage, the average concentration of tartrazine obtained using both methods was compared using a t-test with 2 degrees of freedom and 95% of confidence (ttab = 4.3). This analysis revealed no significant differences between the results from each method. Therefore, the methodology of MSPE is comparable with the reference methodology. Additionally, the MSPE is a robust preconcentration technique that can be coupled even to spectrophotometry or HPLC.

Bottom Line: The tartrazine eluted from the magnetic support was determined by spectrophotometry.Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1).A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Laboratorio 2, Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, Mexico.

ABSTRACT
A method is presented for magnetic solid phase extraction of tartrazine from nonalcoholic beverages. The method involves the extraction and clean-up by activated carbon covered with magnetite dispersed in the sample, followed by the magnetic isolation and desorption of the analyte by basified methanol. The tartrazine eluted from the magnetic support was determined by spectrophotometry. Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1). The method was validated by comparing the results with those obtained by HPLC. A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

No MeSH data available.


Related in: MedlinePlus