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Application of an activated carbon-based support for magnetic solid phase extraction followed by spectrophotometric determination of tartrazine in commercial beverages.

Rodríguez JA, Escamilla-Lara KA, Guevara-Lara A, Miranda JM, Páez-Hernández ME - Int J Anal Chem (2015)

Bottom Line: The tartrazine eluted from the magnetic support was determined by spectrophotometry.Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1).A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Laboratorio 2, Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, Mexico.

ABSTRACT
A method is presented for magnetic solid phase extraction of tartrazine from nonalcoholic beverages. The method involves the extraction and clean-up by activated carbon covered with magnetite dispersed in the sample, followed by the magnetic isolation and desorption of the analyte by basified methanol. The tartrazine eluted from the magnetic support was determined by spectrophotometry. Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1). The method was validated by comparing the results with those obtained by HPLC. A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

No MeSH data available.


Related in: MedlinePlus

Plot of average effects in the tartrazine recovery. VM: sample volume; VE: eluent volume; mC: magnetic modified carbon; and CE: NaOH concentration in the eluent.
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fig5: Plot of average effects in the tartrazine recovery. VM: sample volume; VE: eluent volume; mC: magnetic modified carbon; and CE: NaOH concentration in the eluent.

Mentions: Figure 5 shows a typical graph obtained by plotting the average percentage of recovery for each factor against each of its levels. According to this, the most suitable conditions for tartrazine adsorption-elution were 20 mL sample, 1 mL of eluent, 75 mg of magnetic modified carbon, and a concentration of 0.25 mol L−1 NaOH for the basified methanol solution. These conditions correspond to experiment number 4, which has the highest percentage of dye recovery.


Application of an activated carbon-based support for magnetic solid phase extraction followed by spectrophotometric determination of tartrazine in commercial beverages.

Rodríguez JA, Escamilla-Lara KA, Guevara-Lara A, Miranda JM, Páez-Hernández ME - Int J Anal Chem (2015)

Plot of average effects in the tartrazine recovery. VM: sample volume; VE: eluent volume; mC: magnetic modified carbon; and CE: NaOH concentration in the eluent.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383302&req=5

fig5: Plot of average effects in the tartrazine recovery. VM: sample volume; VE: eluent volume; mC: magnetic modified carbon; and CE: NaOH concentration in the eluent.
Mentions: Figure 5 shows a typical graph obtained by plotting the average percentage of recovery for each factor against each of its levels. According to this, the most suitable conditions for tartrazine adsorption-elution were 20 mL sample, 1 mL of eluent, 75 mg of magnetic modified carbon, and a concentration of 0.25 mol L−1 NaOH for the basified methanol solution. These conditions correspond to experiment number 4, which has the highest percentage of dye recovery.

Bottom Line: The tartrazine eluted from the magnetic support was determined by spectrophotometry.Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1).A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Laboratorio 2, Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184 Mineral de la Reforma, HGO, Mexico.

ABSTRACT
A method is presented for magnetic solid phase extraction of tartrazine from nonalcoholic beverages. The method involves the extraction and clean-up by activated carbon covered with magnetite dispersed in the sample, followed by the magnetic isolation and desorption of the analyte by basified methanol. The tartrazine eluted from the magnetic support was determined by spectrophotometry. Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L(-1), with a limit of detection of 1 mg L(-1). The method was validated by comparing the results with those obtained by HPLC. A precision of <5.0% was obtained in all cases and no significant differences were observed (P < 0.05).

No MeSH data available.


Related in: MedlinePlus