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Alterations in Salivary Proteome following Single Twenty-Minute Session of Yogic Breathing.

Balasubramanian S, Janech MG, Warren GW - Evid Based Complement Alternat Med (2015)

Bottom Line: Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes.There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001).IGLC2 also showed a significant increase in YB group as measured by Western Blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA.

ABSTRACT
Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes. However, alterations after YB at the molecular level are not well established. Twenty healthy volunteers were randomized into two groups (N = 10 per group): YB or attention controls (AC). The YB group performed two YB exercises, each for ten minutes, for a total of twenty minutes in a single session. AC group read a text of their choice for 20 minutes. Saliva was collected at baseline and at 5, 10, 15, and 20 minutes. Using Mass Spectrometry (MS), we initially found that 22 proteins were differentially expressed and then validated deleted in malignant brain tumor-1 (DMBT1) and Ig lambda-2 chain C region (IGLC2) using Western Blotting. DMBT1 was elevated in 7 of YB group by 10-fold and 11-fold at 10 and 15 minutes, respectively, whereas it was undetectable in the time-matched AC group (P < 0.05). There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001). IGLC2 also showed a significant increase in YB group as measured by Western Blotting. These data are the first to demonstrate the feasibility of stimulating and detecting salivary protein biomarkers in response to an acute Yoga exercise. This trial is registered with ClincalTrials.gov NCT02108769.

No MeSH data available.


Related in: MedlinePlus

Increased salivary DMBT1 abundance in Yogic breathing participants. Saliva from Yogic breathing group participants (n = 10) was collected at indicated time points. Equal volumes of saliva were loaded and analyzed by Western Blotting with DMBT1 antibody as described under Methods. DMBT1 band around 220 kDa is shown (a). The same membrane was stained with Ponceau S and shown for loading control (b).
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fig3: Increased salivary DMBT1 abundance in Yogic breathing participants. Saliva from Yogic breathing group participants (n = 10) was collected at indicated time points. Equal volumes of saliva were loaded and analyzed by Western Blotting with DMBT1 antibody as described under Methods. DMBT1 band around 220 kDa is shown (a). The same membrane was stained with Ponceau S and shown for loading control (b).

Mentions: Expression of salivary DMBT1 using Western Blot analysis is shown for all of the samples from YB group (Figure 3(a)) and AC group (Figure 4(a)). Elevated expression of DMBT1 was observed in 7 out of 10 subjects from the YB group and none in the AC group. Elevations in DMBT1 level were observed as early as 5 min and sustained through 15 min in all participants who demonstrated elevated DMBT1 following YB. In 4 of the 7 participants with elevated DMBT1, the increase in expression was reversed by 20 min following YB suggesting that the effects of YB on DMBT1 expression may be acute.


Alterations in Salivary Proteome following Single Twenty-Minute Session of Yogic Breathing.

Balasubramanian S, Janech MG, Warren GW - Evid Based Complement Alternat Med (2015)

Increased salivary DMBT1 abundance in Yogic breathing participants. Saliva from Yogic breathing group participants (n = 10) was collected at indicated time points. Equal volumes of saliva were loaded and analyzed by Western Blotting with DMBT1 antibody as described under Methods. DMBT1 band around 220 kDa is shown (a). The same membrane was stained with Ponceau S and shown for loading control (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383272&req=5

fig3: Increased salivary DMBT1 abundance in Yogic breathing participants. Saliva from Yogic breathing group participants (n = 10) was collected at indicated time points. Equal volumes of saliva were loaded and analyzed by Western Blotting with DMBT1 antibody as described under Methods. DMBT1 band around 220 kDa is shown (a). The same membrane was stained with Ponceau S and shown for loading control (b).
Mentions: Expression of salivary DMBT1 using Western Blot analysis is shown for all of the samples from YB group (Figure 3(a)) and AC group (Figure 4(a)). Elevated expression of DMBT1 was observed in 7 out of 10 subjects from the YB group and none in the AC group. Elevations in DMBT1 level were observed as early as 5 min and sustained through 15 min in all participants who demonstrated elevated DMBT1 following YB. In 4 of the 7 participants with elevated DMBT1, the increase in expression was reversed by 20 min following YB suggesting that the effects of YB on DMBT1 expression may be acute.

Bottom Line: Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes.There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001).IGLC2 also showed a significant increase in YB group as measured by Western Blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA.

ABSTRACT
Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes. However, alterations after YB at the molecular level are not well established. Twenty healthy volunteers were randomized into two groups (N = 10 per group): YB or attention controls (AC). The YB group performed two YB exercises, each for ten minutes, for a total of twenty minutes in a single session. AC group read a text of their choice for 20 minutes. Saliva was collected at baseline and at 5, 10, 15, and 20 minutes. Using Mass Spectrometry (MS), we initially found that 22 proteins were differentially expressed and then validated deleted in malignant brain tumor-1 (DMBT1) and Ig lambda-2 chain C region (IGLC2) using Western Blotting. DMBT1 was elevated in 7 of YB group by 10-fold and 11-fold at 10 and 15 minutes, respectively, whereas it was undetectable in the time-matched AC group (P < 0.05). There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001). IGLC2 also showed a significant increase in YB group as measured by Western Blotting. These data are the first to demonstrate the feasibility of stimulating and detecting salivary protein biomarkers in response to an acute Yoga exercise. This trial is registered with ClincalTrials.gov NCT02108769.

No MeSH data available.


Related in: MedlinePlus