Limits...
Alterations in Salivary Proteome following Single Twenty-Minute Session of Yogic Breathing.

Balasubramanian S, Janech MG, Warren GW - Evid Based Complement Alternat Med (2015)

Bottom Line: Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes.There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001).IGLC2 also showed a significant increase in YB group as measured by Western Blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA.

ABSTRACT
Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes. However, alterations after YB at the molecular level are not well established. Twenty healthy volunteers were randomized into two groups (N = 10 per group): YB or attention controls (AC). The YB group performed two YB exercises, each for ten minutes, for a total of twenty minutes in a single session. AC group read a text of their choice for 20 minutes. Saliva was collected at baseline and at 5, 10, 15, and 20 minutes. Using Mass Spectrometry (MS), we initially found that 22 proteins were differentially expressed and then validated deleted in malignant brain tumor-1 (DMBT1) and Ig lambda-2 chain C region (IGLC2) using Western Blotting. DMBT1 was elevated in 7 of YB group by 10-fold and 11-fold at 10 and 15 minutes, respectively, whereas it was undetectable in the time-matched AC group (P < 0.05). There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001). IGLC2 also showed a significant increase in YB group as measured by Western Blotting. These data are the first to demonstrate the feasibility of stimulating and detecting salivary protein biomarkers in response to an acute Yoga exercise. This trial is registered with ClincalTrials.gov NCT02108769.

No MeSH data available.


Related in: MedlinePlus

CONSORT flowchart. Details of the overall trial design.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4383272&req=5

fig1: CONSORT flowchart. Details of the overall trial design.

Mentions: Enrolled participants were randomized to one of 2 conditions: Yogic breathing (YB) arm versus the Attention Control (AC) arm (see Figure 1 for CONSORT flowchart). Randomization was conducted in collaboration with a biostatistician to ensure equal gender distribution in the 2 experimental groups (YB versus AC). All the participants were tested one-on-one with a trained Yoga instructor. Prior to exercise and sample collection, the Yoga instructor taught each subject how to perform YB.


Alterations in Salivary Proteome following Single Twenty-Minute Session of Yogic Breathing.

Balasubramanian S, Janech MG, Warren GW - Evid Based Complement Alternat Med (2015)

CONSORT flowchart. Details of the overall trial design.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383272&req=5

fig1: CONSORT flowchart. Details of the overall trial design.
Mentions: Enrolled participants were randomized to one of 2 conditions: Yogic breathing (YB) arm versus the Attention Control (AC) arm (see Figure 1 for CONSORT flowchart). Randomization was conducted in collaboration with a biostatistician to ensure equal gender distribution in the 2 experimental groups (YB versus AC). All the participants were tested one-on-one with a trained Yoga instructor. Prior to exercise and sample collection, the Yoga instructor taught each subject how to perform YB.

Bottom Line: Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes.There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001).IGLC2 also showed a significant increase in YB group as measured by Western Blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA.

ABSTRACT
Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes. However, alterations after YB at the molecular level are not well established. Twenty healthy volunteers were randomized into two groups (N = 10 per group): YB or attention controls (AC). The YB group performed two YB exercises, each for ten minutes, for a total of twenty minutes in a single session. AC group read a text of their choice for 20 minutes. Saliva was collected at baseline and at 5, 10, 15, and 20 minutes. Using Mass Spectrometry (MS), we initially found that 22 proteins were differentially expressed and then validated deleted in malignant brain tumor-1 (DMBT1) and Ig lambda-2 chain C region (IGLC2) using Western Blotting. DMBT1 was elevated in 7 of YB group by 10-fold and 11-fold at 10 and 15 minutes, respectively, whereas it was undetectable in the time-matched AC group (P < 0.05). There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001). IGLC2 also showed a significant increase in YB group as measured by Western Blotting. These data are the first to demonstrate the feasibility of stimulating and detecting salivary protein biomarkers in response to an acute Yoga exercise. This trial is registered with ClincalTrials.gov NCT02108769.

No MeSH data available.


Related in: MedlinePlus