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Relationship of tumor marker CA125 and ovarian tumor stem cells: preliminary identification.

Zhang H, Yang Y, Wang Y, Gao X, Wang W, Liu H, He H, Liang Y, Pan K, Wu H, Shi J, Xue H, Liang L, Cai Z, Fan Y, Zhang Y - J Ovarian Res (2015)

Bottom Line: Quantities were judged to be significantly different P < 0.0001.CA125+/ lineage- cells, which may be ovarian cancer stem cells, were the source for tumor recurrence.The strategies designed to target this cell population may lead to more effective therapies.

View Article: PubMed Central - PubMed

Affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 15033217346@163.com.

ABSTRACT

Purpose: The purpose of this study is to identify a prospective association between CA125 and tumorigenic ovarian cancer cells, using the new method of orthotopic transplantation (1).

Method: After making the surgical ovarian cancer specimen into cell suspension, we separated the tumorigenic cells from the nontumorigenic cancer cells based on cell surface marker (cancer antigen CA125 and lineage markers) expression. We developed a SCID mice model in which the CA125+/ lineage- and CA125-/ lineage- cells were injected into ovarian parenchyma by use of a microinjector. As a measure of effectiveness of tumor-forming, tumor weight, abdominal distension, ascites volume and activity, subcutaneous fat were determined or observed. Immunohistochemistry was done to determine tumor cell markers.

Results: We found that the cells of CA125+/ lineage- were able to form new tumors; whereas, an equal quantity of CA125-/lineage- cells failed to form any tumors. The new generated tumor contained additional CA125-/lineage- tumorigenic cells as well as the phenotypically diverse population of nontumorigenic cells. Quantities were judged to be significantly different P < 0.0001.

Conclusion: CA125+/ lineage- cells, which may be ovarian cancer stem cells, were the source for tumor recurrence. The strategies designed to target this cell population may lead to more effective therapies.

No MeSH data available.


Related in: MedlinePlus

The pelvic muscle metastasis.
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Fig12: The pelvic muscle metastasis.

Mentions: As seen in data presented in Table 1, 8 tumors were found in the positive group. As shown in the follow Figures, no tumor was observed in the negative and control groups. Tumorigenic status were showed in Figures 4, 5. Figure 4 is the state of pre-anatomy after tumor formation, Figure 5 is the state after anatomy. Figure 6 illustrated the ovarian tumor on H&E staining of the vaccinated tumor cells (×40). One case had peritoneal metastasis (Figure 7). Figures 8 and 9 showed the immunohistochemical staining of the ovarian tumor for detecting expression of CA125 (brown color). Two cases had liver metastasis, as shown in Figures 10 and 11. Figure 11 was the tumor transferred to liver under the electron microscopy, where a tumor cell was at the upper right, and a solid nucleus liver cell was found at the left bottom. Two cases had pelvic muscle metastasis, (Figure 12). Figure 13 showed the expressions of human CA125 (red light) and mouse antigen (green light) of the ovarian tumor. Analysis by SAS FREQ showed that the difference among the positive groups with different CA125+ /lineage-magnitude was not significant, P=0.4444. The difference between CA125+ /lineage-group and CA125- /lineage-group was statistically significant, (P<0.0001) While the difference between CA125+ /lineage-group and the control group (blank control and control groups) was significant (P<0.0001), and the difference between CA125-/lineage-group and the control group was not significant.Table 1


Relationship of tumor marker CA125 and ovarian tumor stem cells: preliminary identification.

Zhang H, Yang Y, Wang Y, Gao X, Wang W, Liu H, He H, Liang Y, Pan K, Wu H, Shi J, Xue H, Liang L, Cai Z, Fan Y, Zhang Y - J Ovarian Res (2015)

The pelvic muscle metastasis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4383191&req=5

Fig12: The pelvic muscle metastasis.
Mentions: As seen in data presented in Table 1, 8 tumors were found in the positive group. As shown in the follow Figures, no tumor was observed in the negative and control groups. Tumorigenic status were showed in Figures 4, 5. Figure 4 is the state of pre-anatomy after tumor formation, Figure 5 is the state after anatomy. Figure 6 illustrated the ovarian tumor on H&E staining of the vaccinated tumor cells (×40). One case had peritoneal metastasis (Figure 7). Figures 8 and 9 showed the immunohistochemical staining of the ovarian tumor for detecting expression of CA125 (brown color). Two cases had liver metastasis, as shown in Figures 10 and 11. Figure 11 was the tumor transferred to liver under the electron microscopy, where a tumor cell was at the upper right, and a solid nucleus liver cell was found at the left bottom. Two cases had pelvic muscle metastasis, (Figure 12). Figure 13 showed the expressions of human CA125 (red light) and mouse antigen (green light) of the ovarian tumor. Analysis by SAS FREQ showed that the difference among the positive groups with different CA125+ /lineage-magnitude was not significant, P=0.4444. The difference between CA125+ /lineage-group and CA125- /lineage-group was statistically significant, (P<0.0001) While the difference between CA125+ /lineage-group and the control group (blank control and control groups) was significant (P<0.0001), and the difference between CA125-/lineage-group and the control group was not significant.Table 1

Bottom Line: Quantities were judged to be significantly different P < 0.0001.CA125+/ lineage- cells, which may be ovarian cancer stem cells, were the source for tumor recurrence.The strategies designed to target this cell population may lead to more effective therapies.

View Article: PubMed Central - PubMed

Affiliation: Affiliated Hospital of HeBei University, Baoding City, HeBei Province, China. 15033217346@163.com.

ABSTRACT

Purpose: The purpose of this study is to identify a prospective association between CA125 and tumorigenic ovarian cancer cells, using the new method of orthotopic transplantation (1).

Method: After making the surgical ovarian cancer specimen into cell suspension, we separated the tumorigenic cells from the nontumorigenic cancer cells based on cell surface marker (cancer antigen CA125 and lineage markers) expression. We developed a SCID mice model in which the CA125+/ lineage- and CA125-/ lineage- cells were injected into ovarian parenchyma by use of a microinjector. As a measure of effectiveness of tumor-forming, tumor weight, abdominal distension, ascites volume and activity, subcutaneous fat were determined or observed. Immunohistochemistry was done to determine tumor cell markers.

Results: We found that the cells of CA125+/ lineage- were able to form new tumors; whereas, an equal quantity of CA125-/lineage- cells failed to form any tumors. The new generated tumor contained additional CA125-/lineage- tumorigenic cells as well as the phenotypically diverse population of nontumorigenic cells. Quantities were judged to be significantly different P < 0.0001.

Conclusion: CA125+/ lineage- cells, which may be ovarian cancer stem cells, were the source for tumor recurrence. The strategies designed to target this cell population may lead to more effective therapies.

No MeSH data available.


Related in: MedlinePlus