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Experimental study of sutureless vascular anastomosis with use of glued prosthesis in rabbits.

Vokrri L, Qavdarbasha A, Rudari H, Ahmetaj H, Manxhuka-Kërliu S, Hyseni N, Porcu P, Cinquin P, Sessa C - Vasc Health Risk Manag (2015)

Bottom Line: Macroscopically, the BioGlue did not demonstrate any adhesion to the surrounding tissue as it was covered by the vascular prosthesis.This technique may provide a feasible and successful alternative in vascular surgery.However, further long-term studies are necessary to elucidate the break pressure and degree of inflammation at the anastomotic site.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo ; University of Grenoble Alpes/CNRS/TIMC-IMAG UMR 5525 (GMCAO team), Grenoble, 38000, France.

ABSTRACT

Objective: The objective of this study is to explore the feasibility and efficacy of a new technique for sutureless vascular anastomosis, using glued prosthesis, as a sole anastomosis fixation method in rabbits.

Methods: Ten rabbits were randomly selected to conduct the experiment. Five rabbits underwent direct anastomosis of infrarenal abdominal aorta, with glued prosthesis. In five other rabbits, reconstruction was done by sutured anastomosis. All animals were immediately examined by echo-Doppler for patency of anastomosis. The burst pressure of the glued anastomosis was measured and compared with that of a sutured artery. The animals were euthanized, and tissue samples were taken for histological examination immediately after the experiment.

Results: Compared to conventional anastomoses, sutureless vascular anastomoses required shorter time of creation and significantly reduced blood loss (P<5%). There was no significant difference on the average blood flow through the anastomosis between two groups at the end of surgery. All anastomoses with glued prosthesis, examined by echo-Doppler, were patent at the anastomotic site, except one, which was stenosed immediately after surgery. In the control group, except one with stenosis, all conventional anastomoses were patent. Mean burst pressure at the anastomotic site for sutureless anastomoses was lower than in control group. Macroscopically, the BioGlue did not demonstrate any adhesion to the surrounding tissue as it was covered by the vascular prosthesis. Histological examination showed low-grade inflammatory reaction in glued anastomoses versus no inflammatory reaction at the sutured anastomoses.

Conclusion: This technique may provide a feasible and successful alternative in vascular surgery. However, further long-term studies are necessary to elucidate the break pressure and degree of inflammation at the anastomotic site.

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The average blood flow of anastomosis (n=10, P=0.398).
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f6-vhrm-11-211: The average blood flow of anastomosis (n=10, P=0.398).

Mentions: The average blood flow through anastomosis with glued prosthesis at the end of operation was 39±2.55 mL/min and 40.4±2.41 mL/min in the sutureless and sutured anastomoses, respectively, in the control group. The color-Doppler shows the laminar flow and the absence of blood turbulences at the anastomotic site of sutureless aortic anastomosis. One anastomosis by the glued prosthesis was found to be stenosed immediately, probably due to exceeded doses of applied BioGlue. In three other anastomoses, a low degree of inflammation was macroscopically visible. In the control group, all conventional sutured anastomoses were patent. One anastomose showed approximately 50% stenosis, which occurred during suturing (Table 1). By the high magnitude of the t-statistic, we can conclude that the difference among groups is statistically insignificant. Our results have shown that there is no statistically significant difference between the two groups in terms of blood flow through the anastomosis (Figure 6).


Experimental study of sutureless vascular anastomosis with use of glued prosthesis in rabbits.

Vokrri L, Qavdarbasha A, Rudari H, Ahmetaj H, Manxhuka-Kërliu S, Hyseni N, Porcu P, Cinquin P, Sessa C - Vasc Health Risk Manag (2015)

The average blood flow of anastomosis (n=10, P=0.398).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383148&req=5

f6-vhrm-11-211: The average blood flow of anastomosis (n=10, P=0.398).
Mentions: The average blood flow through anastomosis with glued prosthesis at the end of operation was 39±2.55 mL/min and 40.4±2.41 mL/min in the sutureless and sutured anastomoses, respectively, in the control group. The color-Doppler shows the laminar flow and the absence of blood turbulences at the anastomotic site of sutureless aortic anastomosis. One anastomosis by the glued prosthesis was found to be stenosed immediately, probably due to exceeded doses of applied BioGlue. In three other anastomoses, a low degree of inflammation was macroscopically visible. In the control group, all conventional sutured anastomoses were patent. One anastomose showed approximately 50% stenosis, which occurred during suturing (Table 1). By the high magnitude of the t-statistic, we can conclude that the difference among groups is statistically insignificant. Our results have shown that there is no statistically significant difference between the two groups in terms of blood flow through the anastomosis (Figure 6).

Bottom Line: Macroscopically, the BioGlue did not demonstrate any adhesion to the surrounding tissue as it was covered by the vascular prosthesis.This technique may provide a feasible and successful alternative in vascular surgery.However, further long-term studies are necessary to elucidate the break pressure and degree of inflammation at the anastomotic site.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, University Clinical Center of Kosovo, Republic of Kosovo ; Medical Faculty, University of Pristina, Republic of Kosovo ; Veterinary Institute, Pristina, Republic of Kosovo ; University of Grenoble Alpes/CNRS/TIMC-IMAG UMR 5525 (GMCAO team), Grenoble, 38000, France.

ABSTRACT

Objective: The objective of this study is to explore the feasibility and efficacy of a new technique for sutureless vascular anastomosis, using glued prosthesis, as a sole anastomosis fixation method in rabbits.

Methods: Ten rabbits were randomly selected to conduct the experiment. Five rabbits underwent direct anastomosis of infrarenal abdominal aorta, with glued prosthesis. In five other rabbits, reconstruction was done by sutured anastomosis. All animals were immediately examined by echo-Doppler for patency of anastomosis. The burst pressure of the glued anastomosis was measured and compared with that of a sutured artery. The animals were euthanized, and tissue samples were taken for histological examination immediately after the experiment.

Results: Compared to conventional anastomoses, sutureless vascular anastomoses required shorter time of creation and significantly reduced blood loss (P<5%). There was no significant difference on the average blood flow through the anastomosis between two groups at the end of surgery. All anastomoses with glued prosthesis, examined by echo-Doppler, were patent at the anastomotic site, except one, which was stenosed immediately after surgery. In the control group, except one with stenosis, all conventional anastomoses were patent. Mean burst pressure at the anastomotic site for sutureless anastomoses was lower than in control group. Macroscopically, the BioGlue did not demonstrate any adhesion to the surrounding tissue as it was covered by the vascular prosthesis. Histological examination showed low-grade inflammatory reaction in glued anastomoses versus no inflammatory reaction at the sutured anastomoses.

Conclusion: This technique may provide a feasible and successful alternative in vascular surgery. However, further long-term studies are necessary to elucidate the break pressure and degree of inflammation at the anastomotic site.

Show MeSH
Related in: MedlinePlus