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Bioorganic fertilizer enhances soil suppressive capacity against bacterial wilt of tomato.

Liu L, Sun C, Liu S, Chai R, Huang W, Liu X, Tang C, Zhang Y - PLoS ONE (2015)

Bottom Line: In this study, a novel bioorganic fertilizer revealed a higher suppressive ability against bacterial wilt compared with several soil management methods in the field over four growing seasons from March 2011 to July 2013.Redundancy analysis showed that bacterial community diversity and richness negatively related with bacterial wilt suppressiveness, while fungal community richness positively correlated with R. solanacearum population.We concluded that the alteration of soil physicochemical and biological properties in soil treated with the bioorganic fertilizer induced the soil suppressiveness against tomato bacterial wilt.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China.

ABSTRACT
Tomato bacterial wilt caused by Ralstonia solanacearum is one of the most destructive soil-borne diseases. Many strategies have been taken to improve soil suppressiveness against this destructive disease, but limited success has been achieved. In this study, a novel bioorganic fertilizer revealed a higher suppressive ability against bacterial wilt compared with several soil management methods in the field over four growing seasons from March 2011 to July 2013. The application of the bioorganic fertilizer significantly (P<0.05) reduced disease incidence of tomato and increased fruit yields in four independent trials. The association among the level of disease incidence, soil physicochemical and biological properties was investigated. The soil treated with the bioorganic fertilizer increased soil pH value, electric conductivity, organic carbon, NH4+-N, NO3--N and available K content, microbial activities and microbial biomass carbon content, which were positively related with soil suppressiveness. Bacterial and actinomycete populations assessed using classical plate counts were highest, whereas R. solanacearum and fungal populations were lowest in soil applied with the bioorganic fertilizer. Microbial community diversity and richness were assessed using denaturing gel gradient electrophoresis profile analysis. The soil treated with the bioorganic fertilizer exhibited higher bacterial community diversity but lower fungal community diversity. Redundancy analysis showed that bacterial community diversity and richness negatively related with bacterial wilt suppressiveness, while fungal community richness positively correlated with R. solanacearum population. We concluded that the alteration of soil physicochemical and biological properties in soil treated with the bioorganic fertilizer induced the soil suppressiveness against tomato bacterial wilt.

No MeSH data available.


Population of Ralstonia solanacearum in soil and disease incidence over time in Test 1 (a and c) and Test 2 (b and d).The dotted lines and ◇ represent the mean max temperature in the above time periods in different trials. CK: NPK fertilizer; O: NPK fertilizer + organic fertilizer; B: NPK fertilizer + bioorganic fertilizer; S: NPK fertilizer + soil disinfection; S+B: soil disinfection + NPK fertilizer + bioorganic fertilizer. * indicates the significant difference between the treatments at the 0.05 probability level according to the Duncan’s test.
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pone.0121304.g001: Population of Ralstonia solanacearum in soil and disease incidence over time in Test 1 (a and c) and Test 2 (b and d).The dotted lines and ◇ represent the mean max temperature in the above time periods in different trials. CK: NPK fertilizer; O: NPK fertilizer + organic fertilizer; B: NPK fertilizer + bioorganic fertilizer; S: NPK fertilizer + soil disinfection; S+B: soil disinfection + NPK fertilizer + bioorganic fertilizer. * indicates the significant difference between the treatments at the 0.05 probability level according to the Duncan’s test.

Mentions: The controlling efficacy of tomato bacterial wilt by different fertilizers/amendments at different growth stages was shown in Fig 1. R. solanacearum population in the B treatment was decreased by 23–24% in Trial 1 and by 8–36% in Trial 2 during the entire experiment period compared with the control (Fig 1A), while no difference was observed between the O and control treatments in both trial periods.


Bioorganic fertilizer enhances soil suppressive capacity against bacterial wilt of tomato.

Liu L, Sun C, Liu S, Chai R, Huang W, Liu X, Tang C, Zhang Y - PLoS ONE (2015)

Population of Ralstonia solanacearum in soil and disease incidence over time in Test 1 (a and c) and Test 2 (b and d).The dotted lines and ◇ represent the mean max temperature in the above time periods in different trials. CK: NPK fertilizer; O: NPK fertilizer + organic fertilizer; B: NPK fertilizer + bioorganic fertilizer; S: NPK fertilizer + soil disinfection; S+B: soil disinfection + NPK fertilizer + bioorganic fertilizer. * indicates the significant difference between the treatments at the 0.05 probability level according to the Duncan’s test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4382293&req=5

pone.0121304.g001: Population of Ralstonia solanacearum in soil and disease incidence over time in Test 1 (a and c) and Test 2 (b and d).The dotted lines and ◇ represent the mean max temperature in the above time periods in different trials. CK: NPK fertilizer; O: NPK fertilizer + organic fertilizer; B: NPK fertilizer + bioorganic fertilizer; S: NPK fertilizer + soil disinfection; S+B: soil disinfection + NPK fertilizer + bioorganic fertilizer. * indicates the significant difference between the treatments at the 0.05 probability level according to the Duncan’s test.
Mentions: The controlling efficacy of tomato bacterial wilt by different fertilizers/amendments at different growth stages was shown in Fig 1. R. solanacearum population in the B treatment was decreased by 23–24% in Trial 1 and by 8–36% in Trial 2 during the entire experiment period compared with the control (Fig 1A), while no difference was observed between the O and control treatments in both trial periods.

Bottom Line: In this study, a novel bioorganic fertilizer revealed a higher suppressive ability against bacterial wilt compared with several soil management methods in the field over four growing seasons from March 2011 to July 2013.Redundancy analysis showed that bacterial community diversity and richness negatively related with bacterial wilt suppressiveness, while fungal community richness positively correlated with R. solanacearum population.We concluded that the alteration of soil physicochemical and biological properties in soil treated with the bioorganic fertilizer induced the soil suppressiveness against tomato bacterial wilt.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China.

ABSTRACT
Tomato bacterial wilt caused by Ralstonia solanacearum is one of the most destructive soil-borne diseases. Many strategies have been taken to improve soil suppressiveness against this destructive disease, but limited success has been achieved. In this study, a novel bioorganic fertilizer revealed a higher suppressive ability against bacterial wilt compared with several soil management methods in the field over four growing seasons from March 2011 to July 2013. The application of the bioorganic fertilizer significantly (P<0.05) reduced disease incidence of tomato and increased fruit yields in four independent trials. The association among the level of disease incidence, soil physicochemical and biological properties was investigated. The soil treated with the bioorganic fertilizer increased soil pH value, electric conductivity, organic carbon, NH4+-N, NO3--N and available K content, microbial activities and microbial biomass carbon content, which were positively related with soil suppressiveness. Bacterial and actinomycete populations assessed using classical plate counts were highest, whereas R. solanacearum and fungal populations were lowest in soil applied with the bioorganic fertilizer. Microbial community diversity and richness were assessed using denaturing gel gradient electrophoresis profile analysis. The soil treated with the bioorganic fertilizer exhibited higher bacterial community diversity but lower fungal community diversity. Redundancy analysis showed that bacterial community diversity and richness negatively related with bacterial wilt suppressiveness, while fungal community richness positively correlated with R. solanacearum population. We concluded that the alteration of soil physicochemical and biological properties in soil treated with the bioorganic fertilizer induced the soil suppressiveness against tomato bacterial wilt.

No MeSH data available.