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In vivo expression patterns of microRNAs of Gallid herpesvirus 2 (GaHV-2) during the virus life cycle and development of Marek's disease lymphomas.

Zhao P, Li XJ, Teng M, Dang L, Yu ZH, Chi JQ, Su JW, Zhang GP, Luo J - Virus Genes (2015)

Bottom Line: The biological role of most GaHV-2 miRNAs remains unclear.According to their distinct in vivo expression patterns, the GaHV-2 miRNAs can be divided into three groups: 12 miRNAs in group I, including miR-M4-5p, displayed a typical expression pattern potentially correlated to the latent, late cytolytic, and/or the proliferative phases in the cycle of GaHV-2 pathogenesis; group II consisting of another 12 miRNAs with expression correlated to the early cytolytic and/or latent phases in GaHV-2's life cycle; while the other two miRNAs in group III showed no identical expression features.Our findings may provide meaningful clues in the search for further potential functions of viral miRNAs in GaHV-2 biology.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China.

ABSTRACT
In the past decade, a large number of microRNAs (miRNAs) have been identified in the viral genome of Gallid herpesvirus 2 (GaHV-2), which is historically known as Marek's disease virus type 1. The biological role of most GaHV-2 miRNAs remains unclear. In the present study, we have performed an overall gene expression profile of GaHV-2 miRNAs during the virus life cycle at each phase of the developing disease, a highly contagious, lymphoproliferative disorder, and neoplastic immunosuppressive disease of poultry known as the Marek's disease. According to their distinct in vivo expression patterns, the GaHV-2 miRNAs can be divided into three groups: 12 miRNAs in group I, including miR-M4-5p, displayed a typical expression pattern potentially correlated to the latent, late cytolytic, and/or the proliferative phases in the cycle of GaHV-2 pathogenesis; group II consisting of another 12 miRNAs with expression correlated to the early cytolytic and/or latent phases in GaHV-2's life cycle; while the other two miRNAs in group III showed no identical expression features. Our findings may provide meaningful clues in the search for further potential functions of viral miRNAs in GaHV-2 biology.

No MeSH data available.


Related in: MedlinePlus

Relative expression levels of the GaHV-2 protein-coding genes analyzed by qRT-PCR. agB, the gene of glycoprotein B; bICP4, the gene of infected cell protein 4; cpp38, the gene of 38 KD phosphorylated protein; dMeq, the oncogene of MDV EcoRI-Q. The endogenous chicken GAPDH gene was used as a standard and the relative quantification of mRNA expression was calculated with the  method. Columns represent the mean of relative expression levels in the spleens of three randomly selected birds, determined in triplicate. Error bars indicate 1× SD. The star indicates significant difference (p < 0.05) compared to that determined at 3 dpi
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Fig1: Relative expression levels of the GaHV-2 protein-coding genes analyzed by qRT-PCR. agB, the gene of glycoprotein B; bICP4, the gene of infected cell protein 4; cpp38, the gene of 38 KD phosphorylated protein; dMeq, the oncogene of MDV EcoRI-Q. The endogenous chicken GAPDH gene was used as a standard and the relative quantification of mRNA expression was calculated with the method. Columns represent the mean of relative expression levels in the spleens of three randomly selected birds, determined in triplicate. Error bars indicate 1× SD. The star indicates significant difference (p < 0.05) compared to that determined at 3 dpi

Mentions: Using the pattern of disease progress described in the ‘Cornell Model’ [25, 26], the GaHV-2 protein-coding genes, including gB, ICP4, pp38, and meq, gave expression patterns as expected in the spleens of GX0101-challenged birds during the early stages of GaHV-2 infection (Fig. 1). The relative expression levels of all four viral genes increased first at 7 dpi (the early cytolytic phase), decreased at 14 dpi (the latent phase), and then peaked at 21 dpi (the late cytolytic phase). At the late time period of 30–60 dpi (the transformation and proliferative phase), the expressions of the genes of gB, ICP4, and pp38 were gradually reduced (Fig. 1a–c) but meq remained expressed at a higher level (Fig. 1d), reflecting its role as a viral oncogene [26].Fig. 1


In vivo expression patterns of microRNAs of Gallid herpesvirus 2 (GaHV-2) during the virus life cycle and development of Marek's disease lymphomas.

Zhao P, Li XJ, Teng M, Dang L, Yu ZH, Chi JQ, Su JW, Zhang GP, Luo J - Virus Genes (2015)

Relative expression levels of the GaHV-2 protein-coding genes analyzed by qRT-PCR. agB, the gene of glycoprotein B; bICP4, the gene of infected cell protein 4; cpp38, the gene of 38 KD phosphorylated protein; dMeq, the oncogene of MDV EcoRI-Q. The endogenous chicken GAPDH gene was used as a standard and the relative quantification of mRNA expression was calculated with the  method. Columns represent the mean of relative expression levels in the spleens of three randomly selected birds, determined in triplicate. Error bars indicate 1× SD. The star indicates significant difference (p < 0.05) compared to that determined at 3 dpi
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4381040&req=5

Fig1: Relative expression levels of the GaHV-2 protein-coding genes analyzed by qRT-PCR. agB, the gene of glycoprotein B; bICP4, the gene of infected cell protein 4; cpp38, the gene of 38 KD phosphorylated protein; dMeq, the oncogene of MDV EcoRI-Q. The endogenous chicken GAPDH gene was used as a standard and the relative quantification of mRNA expression was calculated with the method. Columns represent the mean of relative expression levels in the spleens of three randomly selected birds, determined in triplicate. Error bars indicate 1× SD. The star indicates significant difference (p < 0.05) compared to that determined at 3 dpi
Mentions: Using the pattern of disease progress described in the ‘Cornell Model’ [25, 26], the GaHV-2 protein-coding genes, including gB, ICP4, pp38, and meq, gave expression patterns as expected in the spleens of GX0101-challenged birds during the early stages of GaHV-2 infection (Fig. 1). The relative expression levels of all four viral genes increased first at 7 dpi (the early cytolytic phase), decreased at 14 dpi (the latent phase), and then peaked at 21 dpi (the late cytolytic phase). At the late time period of 30–60 dpi (the transformation and proliferative phase), the expressions of the genes of gB, ICP4, and pp38 were gradually reduced (Fig. 1a–c) but meq remained expressed at a higher level (Fig. 1d), reflecting its role as a viral oncogene [26].Fig. 1

Bottom Line: The biological role of most GaHV-2 miRNAs remains unclear.According to their distinct in vivo expression patterns, the GaHV-2 miRNAs can be divided into three groups: 12 miRNAs in group I, including miR-M4-5p, displayed a typical expression pattern potentially correlated to the latent, late cytolytic, and/or the proliferative phases in the cycle of GaHV-2 pathogenesis; group II consisting of another 12 miRNAs with expression correlated to the early cytolytic and/or latent phases in GaHV-2's life cycle; while the other two miRNAs in group III showed no identical expression features.Our findings may provide meaningful clues in the search for further potential functions of viral miRNAs in GaHV-2 biology.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, People's Republic of China.

ABSTRACT
In the past decade, a large number of microRNAs (miRNAs) have been identified in the viral genome of Gallid herpesvirus 2 (GaHV-2), which is historically known as Marek's disease virus type 1. The biological role of most GaHV-2 miRNAs remains unclear. In the present study, we have performed an overall gene expression profile of GaHV-2 miRNAs during the virus life cycle at each phase of the developing disease, a highly contagious, lymphoproliferative disorder, and neoplastic immunosuppressive disease of poultry known as the Marek's disease. According to their distinct in vivo expression patterns, the GaHV-2 miRNAs can be divided into three groups: 12 miRNAs in group I, including miR-M4-5p, displayed a typical expression pattern potentially correlated to the latent, late cytolytic, and/or the proliferative phases in the cycle of GaHV-2 pathogenesis; group II consisting of another 12 miRNAs with expression correlated to the early cytolytic and/or latent phases in GaHV-2's life cycle; while the other two miRNAs in group III showed no identical expression features. Our findings may provide meaningful clues in the search for further potential functions of viral miRNAs in GaHV-2 biology.

No MeSH data available.


Related in: MedlinePlus