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Characterization of human bladder cell membrane during cancer transformation.

Dobrzyńska I, Szachowicz-Petelska B, Darewicz B, Figaszewski ZA - J. Membr. Biol. (2015)

Bottom Line: The surface charge density of human bladder cell membranes was determined using electrophoresis.Our results show that cancer transformation is associated with increased phospholipid levels and a decreased level of integral proteins.In conclusion, this study demonstrates that cell membrane structure and function are modified in bladder cancer cells and that further work in this area is warranted.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4, 15-443, Białystok, Poland, izadob@uwb.edu.pl.

ABSTRACT
Phenomena associated with changes in cell membranes are thought to play an important role in the cancer transformation. We hypothesized that the electrical charge of tumor cells can indirectly represent membrane-based changes that have occurred during cell transformation and may indicate tumor cell status. Here, we describe work showing that phospholipids, proteins content, and electric charge, are all altered in the cell membranes of pT2 stage/grade G3 bladder cancer. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were identified using high-performance liquid chromatography. Protein composition was determined using selective hydrolysis of isolated bladder cell membrane proteins and peptide resolution. The surface charge density of human bladder cell membranes was determined using electrophoresis. Our results show that cancer transformation is associated with increased phospholipid levels and a decreased level of integral proteins. Moreover, the process of cancer transformation significantly enhanced changes in the surface charge density of the human bladder cell membrane. In conclusion, this study demonstrates that cell membrane structure and function are modified in bladder cancer cells and that further work in this area is warranted.

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The dependence of surface charge density on pH of non-neoplastic and bladder cancer cells from several patients
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Related In: Results  -  Collection


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Fig2: The dependence of surface charge density on pH of non-neoplastic and bladder cancer cells from several patients

Mentions: The effects of surface charge density on pH are similar between normal and bladder cancer cell membranes (Fig. 2). There is an increase in positive surface charge density at low pH values but only up to a point. Conversely at high pH values, the negative charge of the cells is increased until it too reaches a plateau. Overall, compared to unaffected cells, human bladder cancer cells are more negatively charged at low pH values, and more positively charged at high pH values.Fig. 2


Characterization of human bladder cell membrane during cancer transformation.

Dobrzyńska I, Szachowicz-Petelska B, Darewicz B, Figaszewski ZA - J. Membr. Biol. (2015)

The dependence of surface charge density on pH of non-neoplastic and bladder cancer cells from several patients
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4381039&req=5

Fig2: The dependence of surface charge density on pH of non-neoplastic and bladder cancer cells from several patients
Mentions: The effects of surface charge density on pH are similar between normal and bladder cancer cell membranes (Fig. 2). There is an increase in positive surface charge density at low pH values but only up to a point. Conversely at high pH values, the negative charge of the cells is increased until it too reaches a plateau. Overall, compared to unaffected cells, human bladder cancer cells are more negatively charged at low pH values, and more positively charged at high pH values.Fig. 2

Bottom Line: The surface charge density of human bladder cell membranes was determined using electrophoresis.Our results show that cancer transformation is associated with increased phospholipid levels and a decreased level of integral proteins.In conclusion, this study demonstrates that cell membrane structure and function are modified in bladder cancer cells and that further work in this area is warranted.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4, 15-443, Białystok, Poland, izadob@uwb.edu.pl.

ABSTRACT
Phenomena associated with changes in cell membranes are thought to play an important role in the cancer transformation. We hypothesized that the electrical charge of tumor cells can indirectly represent membrane-based changes that have occurred during cell transformation and may indicate tumor cell status. Here, we describe work showing that phospholipids, proteins content, and electric charge, are all altered in the cell membranes of pT2 stage/grade G3 bladder cancer. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were identified using high-performance liquid chromatography. Protein composition was determined using selective hydrolysis of isolated bladder cell membrane proteins and peptide resolution. The surface charge density of human bladder cell membranes was determined using electrophoresis. Our results show that cancer transformation is associated with increased phospholipid levels and a decreased level of integral proteins. Moreover, the process of cancer transformation significantly enhanced changes in the surface charge density of the human bladder cell membrane. In conclusion, this study demonstrates that cell membrane structure and function are modified in bladder cancer cells and that further work in this area is warranted.

Show MeSH
Related in: MedlinePlus