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Oocyte vitrification modifies nucleolar remodeling and zygote kinetics-a sibling study.

Chamayou S, Romano S, Alecci C, Storaci G, Ragolia C, Palagiano A, Guglielmino A - J. Assist. Reprod. Genet. (2015)

Bottom Line: Our aim was to study the effect of oocyte vitrification on zygote-embryo kinetics (pl).A 44-hours time-lapse analysis, from the time of ICSI (t0), of 179 fertilized fresh oocytes was compared to 168 fertilized sibling vitrified/warmed oocytes.Therefore, the predictive morphokinetic parameters on embryo competence found from fresh oocytes must be revised when applied on embryos from vitrified/warmed oocytes.

View Article: PubMed Central - PubMed

Affiliation: Unità Di Medicina della Riproduzione - Istituto HERA, via Barriera del Bosco n. 51/53, 95030, Sant'Agata Li Battiati, Catania, Italy, s.chamayou@yahoo.fr.

ABSTRACT

Purpose: Oocyte vitrification does not affect embryo quality after oocyte warming, making this method effective in the preservation of female fertility. Morphokinetic parameters can be used to predict the competence of an embryo produced from fresh oocytes. Our aim was to study the effect of oocyte vitrification on zygote-embryo kinetics (pl).

Methods: The embryo-kinetics of fresh and sibling vitrified/warmed oocytes were compared to determine the consequences of oocyte preservation on the timing of embryo development. A 44-hours time-lapse analysis, from the time of ICSI (t0), of 179 fertilized fresh oocytes was compared to 168 fertilized sibling vitrified/warmed oocytes.

Results: Oocyte vitrification accelerated pronuclear disappearance, one-cell stage timing and modified nucleoli activity by increasing their number and decreasing their diameter at the zygote stage. In contrast, embryo kinetics during cleavage were similar to those observed for fresh sibling oocytes based on the parameters examined in this study.

Conclusions: At the zygote stage, oocyte vitrification induces changes in pronuclei stability, probably due to pronuclei envelop instability as well as modifications in nucleoli functionality. Therefore, the predictive morphokinetic parameters on embryo competence found from fresh oocytes must be revised when applied on embryos from vitrified/warmed oocytes.

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Related in: MedlinePlus

Embryokinetic from fresh oocytes and sibling vitrified/warmed oocytes from time 0 to 44 h
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4380896&req=5

Fig1: Embryokinetic from fresh oocytes and sibling vitrified/warmed oocytes from time 0 to 44 h

Mentions: The morphokinetic parameters of zygotes and embryos from fresh and vitrified/warmed oocytes in the studied population are shown in Fig. 1.Fig. 1


Oocyte vitrification modifies nucleolar remodeling and zygote kinetics-a sibling study.

Chamayou S, Romano S, Alecci C, Storaci G, Ragolia C, Palagiano A, Guglielmino A - J. Assist. Reprod. Genet. (2015)

Embryokinetic from fresh oocytes and sibling vitrified/warmed oocytes from time 0 to 44 h
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4380896&req=5

Fig1: Embryokinetic from fresh oocytes and sibling vitrified/warmed oocytes from time 0 to 44 h
Mentions: The morphokinetic parameters of zygotes and embryos from fresh and vitrified/warmed oocytes in the studied population are shown in Fig. 1.Fig. 1

Bottom Line: Our aim was to study the effect of oocyte vitrification on zygote-embryo kinetics (pl).A 44-hours time-lapse analysis, from the time of ICSI (t0), of 179 fertilized fresh oocytes was compared to 168 fertilized sibling vitrified/warmed oocytes.Therefore, the predictive morphokinetic parameters on embryo competence found from fresh oocytes must be revised when applied on embryos from vitrified/warmed oocytes.

View Article: PubMed Central - PubMed

Affiliation: Unità Di Medicina della Riproduzione - Istituto HERA, via Barriera del Bosco n. 51/53, 95030, Sant'Agata Li Battiati, Catania, Italy, s.chamayou@yahoo.fr.

ABSTRACT

Purpose: Oocyte vitrification does not affect embryo quality after oocyte warming, making this method effective in the preservation of female fertility. Morphokinetic parameters can be used to predict the competence of an embryo produced from fresh oocytes. Our aim was to study the effect of oocyte vitrification on zygote-embryo kinetics (pl).

Methods: The embryo-kinetics of fresh and sibling vitrified/warmed oocytes were compared to determine the consequences of oocyte preservation on the timing of embryo development. A 44-hours time-lapse analysis, from the time of ICSI (t0), of 179 fertilized fresh oocytes was compared to 168 fertilized sibling vitrified/warmed oocytes.

Results: Oocyte vitrification accelerated pronuclear disappearance, one-cell stage timing and modified nucleoli activity by increasing their number and decreasing their diameter at the zygote stage. In contrast, embryo kinetics during cleavage were similar to those observed for fresh sibling oocytes based on the parameters examined in this study.

Conclusions: At the zygote stage, oocyte vitrification induces changes in pronuclei stability, probably due to pronuclei envelop instability as well as modifications in nucleoli functionality. Therefore, the predictive morphokinetic parameters on embryo competence found from fresh oocytes must be revised when applied on embryos from vitrified/warmed oocytes.

Show MeSH
Related in: MedlinePlus