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Alterations in oral [1-(14)C] 18:1n-9 distribution in lean wild-type and genetically obese (ob/ob) mice.

Wang X, Feng J, Yu C, Shen QW, Wang Y - PLoS ONE (2015)

Bottom Line: Altered FA distribution in obese individuals is poorly understood.The (14)C concentration was constant in adipose tissue and muscle of obese mice from 4 h to 168 h. (14)C-label content in adipose tissue was significantly affected by genotype, whereas muscle (14)C-label content was affected by genotype, time and the interaction between genotype and time.Gene expression suggested acyl CoA binding protein and fatty acid binding protein are important for FA distribution in adipose tissue and muscle.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Hangzhou, Zhejiang, P. R. China.

ABSTRACT
Obesity may result from altered fatty acid (FA) disposal. Altered FA distribution in obese individuals is poorly understood. Lean wild-type C57BL/6J and obese C57BL/6Job/ob mice received an oral dose of [1-(14)C]18:1n-9 (oleic acid), and the radioactivity in tissues was evaluated at various time points. The (14)C concentration decreased rapidly in gastrointestinal tract but gradually increased and peaked at 96 h in adipose tissue, muscle and skin in lean mice. The (14)C concentration was constant in adipose tissue and muscle of obese mice from 4 h to 168 h. (14)C-label content in adipose tissue was significantly affected by genotype, whereas muscle (14)C-label content was affected by genotype, time and the interaction between genotype and time. There was higher total (14)C retention (47.7%) in obese mice than in lean mice (9.0%) at 168 h (P<0.05). The (14)C concentrations in the soleus and gastrocnemius muscle were higher in obese mice than in lean mice (P<0.05). Perirenal adipose tissue contained the highest (14)C content in lean mice, whereas subcutaneous adipose tissue (SAT) had the highest (14)C content and accounted for the largest proportion of total radioactivity among fat depots in obese mice. More lipid radioactivity was recovered as TAG in SAT from obese mice than from lean mice (P<0.05). Gene expression suggested acyl CoA binding protein and fatty acid binding protein are important for FA distribution in adipose tissue and muscle. The FA distribution in major tissues was altered in ob/ob mice, perhaps contributing to obesity. Understanding the disparity in FA disposal between lean and obese mice may reveal novel targets for the treatment and prevention of obesity.

No MeSH data available.


Related in: MedlinePlus

Comparative study of the mRNA expression of ACSL1, FAT, ACBP, FABP3, and FATP1 in the EDL, GAS and SOL between lean and obese mice.The data are presented as the mean ± SEM (n = 3). Asterisks (*) indicate significant differences between lean and obese mice (P<0.05). ACSL, long-chain fatty acyl-CoA synthetase; FAT, fatty acid translocase; ACBP, acyl CoA binding protein; FABP, fatty acid binding protein; FATP, fatty acid transport protein, EDL, extensor digitorum longus; GAS, gastrocnemius; SOL, soleus.
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pone.0122028.g004: Comparative study of the mRNA expression of ACSL1, FAT, ACBP, FABP3, and FATP1 in the EDL, GAS and SOL between lean and obese mice.The data are presented as the mean ± SEM (n = 3). Asterisks (*) indicate significant differences between lean and obese mice (P<0.05). ACSL, long-chain fatty acyl-CoA synthetase; FAT, fatty acid translocase; ACBP, acyl CoA binding protein; FABP, fatty acid binding protein; FATP, fatty acid transport protein, EDL, extensor digitorum longus; GAS, gastrocnemius; SOL, soleus.

Mentions: Alterations in FA uptake are important for the regulation of FA disposal; therefore, the expression of genes involved in LCFA uptake in muscle was analyzed. The gene expression of ACSL1, FATP1, FAT/CD36, ACBP and FABP3 was highest in the SOL of lean and obese mice (S1 Fig). The SOL and GAS exhibited significantly higher expression levels of ACSL1, FAT/CD36, ACBP and FABP3 in obese mice than in lean mice (P<0.05, Fig 4). ACSL1 and FAT/CD36 expression was significantly lower in the EDL from obese mice than from lean mice (P<0.05, Fig 4).


Alterations in oral [1-(14)C] 18:1n-9 distribution in lean wild-type and genetically obese (ob/ob) mice.

Wang X, Feng J, Yu C, Shen QW, Wang Y - PLoS ONE (2015)

Comparative study of the mRNA expression of ACSL1, FAT, ACBP, FABP3, and FATP1 in the EDL, GAS and SOL between lean and obese mice.The data are presented as the mean ± SEM (n = 3). Asterisks (*) indicate significant differences between lean and obese mice (P<0.05). ACSL, long-chain fatty acyl-CoA synthetase; FAT, fatty acid translocase; ACBP, acyl CoA binding protein; FABP, fatty acid binding protein; FATP, fatty acid transport protein, EDL, extensor digitorum longus; GAS, gastrocnemius; SOL, soleus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380473&req=5

pone.0122028.g004: Comparative study of the mRNA expression of ACSL1, FAT, ACBP, FABP3, and FATP1 in the EDL, GAS and SOL between lean and obese mice.The data are presented as the mean ± SEM (n = 3). Asterisks (*) indicate significant differences between lean and obese mice (P<0.05). ACSL, long-chain fatty acyl-CoA synthetase; FAT, fatty acid translocase; ACBP, acyl CoA binding protein; FABP, fatty acid binding protein; FATP, fatty acid transport protein, EDL, extensor digitorum longus; GAS, gastrocnemius; SOL, soleus.
Mentions: Alterations in FA uptake are important for the regulation of FA disposal; therefore, the expression of genes involved in LCFA uptake in muscle was analyzed. The gene expression of ACSL1, FATP1, FAT/CD36, ACBP and FABP3 was highest in the SOL of lean and obese mice (S1 Fig). The SOL and GAS exhibited significantly higher expression levels of ACSL1, FAT/CD36, ACBP and FABP3 in obese mice than in lean mice (P<0.05, Fig 4). ACSL1 and FAT/CD36 expression was significantly lower in the EDL from obese mice than from lean mice (P<0.05, Fig 4).

Bottom Line: Altered FA distribution in obese individuals is poorly understood.The (14)C concentration was constant in adipose tissue and muscle of obese mice from 4 h to 168 h. (14)C-label content in adipose tissue was significantly affected by genotype, whereas muscle (14)C-label content was affected by genotype, time and the interaction between genotype and time.Gene expression suggested acyl CoA binding protein and fatty acid binding protein are important for FA distribution in adipose tissue and muscle.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Hangzhou, Zhejiang, P. R. China.

ABSTRACT
Obesity may result from altered fatty acid (FA) disposal. Altered FA distribution in obese individuals is poorly understood. Lean wild-type C57BL/6J and obese C57BL/6Job/ob mice received an oral dose of [1-(14)C]18:1n-9 (oleic acid), and the radioactivity in tissues was evaluated at various time points. The (14)C concentration decreased rapidly in gastrointestinal tract but gradually increased and peaked at 96 h in adipose tissue, muscle and skin in lean mice. The (14)C concentration was constant in adipose tissue and muscle of obese mice from 4 h to 168 h. (14)C-label content in adipose tissue was significantly affected by genotype, whereas muscle (14)C-label content was affected by genotype, time and the interaction between genotype and time. There was higher total (14)C retention (47.7%) in obese mice than in lean mice (9.0%) at 168 h (P<0.05). The (14)C concentrations in the soleus and gastrocnemius muscle were higher in obese mice than in lean mice (P<0.05). Perirenal adipose tissue contained the highest (14)C content in lean mice, whereas subcutaneous adipose tissue (SAT) had the highest (14)C content and accounted for the largest proportion of total radioactivity among fat depots in obese mice. More lipid radioactivity was recovered as TAG in SAT from obese mice than from lean mice (P<0.05). Gene expression suggested acyl CoA binding protein and fatty acid binding protein are important for FA distribution in adipose tissue and muscle. The FA distribution in major tissues was altered in ob/ob mice, perhaps contributing to obesity. Understanding the disparity in FA disposal between lean and obese mice may reveal novel targets for the treatment and prevention of obesity.

No MeSH data available.


Related in: MedlinePlus