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Prognostic significance of MYH9 expression in resected non-small cell lung cancer.

Katono K, Sato Y, Jiang SX, Kobayashi M, Nagashio R, Ryuge S, Fukuda E, Goshima N, Satoh Y, Saegusa M, Masuda N - PLoS ONE (2015)

Bottom Line: Recently, MYH9 has been thought to be associated with cancer cell migration, invasion, and metastasis.Kaplan-Meier survival analysis and Cox proportional hazards models were used to estimate the effect of MYH9 expression on survival.MYH9 expression was significantly correlated with the adenocarcinoma histology (P = 0.014), poorer differentiation ((P = 0.033), intratumoral vascular invasion and lymphatic invasion ((P = 0.013 and P = 0.045 respectively), and a poorer prognosis ((P = 0.032).

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, School of Medicine, Kitasato University, Kanagawa, Japan.

ABSTRACT

Introduction: Myosin-9 (MYH9) belongs to the myosin superfamily of actin-binding motor protein. Recently, MYH9 has been thought to be associated with cancer cell migration, invasion, and metastasis. The aims of this study were to immunohistochemically examine MYH9 expression in surgically resected non-small cell lung cancer (NSCLC), and evaluate its correlations with clinicopathological parameters and the prognosis of patients.

Methods: MYH9 expression was immunohistochemically studied in 266 consecutive resected NSCLCs, and its associations with clinicopathological parameters were evaluated. Kaplan-Meier survival analysis and Cox proportional hazards models were used to estimate the effect of MYH9 expression on survival.

Results: MYH9 expression was detected in 102 of 266 (38.3%) NSCLCs. MYH9 expression was significantly correlated with the adenocarcinoma histology (P = 0.014), poorer differentiation ((P = 0.033), intratumoral vascular invasion and lymphatic invasion ((P = 0.013 and P = 0.045 respectively), and a poorer prognosis ((P = 0.032). In addition, multivariable analysis revealed that MYH9 expression independently predicted a poorer survival (HR, 2.15; 95%CI, 1.17-3.92; (P = 0.01).

Conclusion: The present study revealed that MYH9 is expressed in a subset of NSCLC with a more malignant nature, and its expression is an indicator of a poorer survival probability.

No MeSH data available.


Related in: MedlinePlus

Immunoprecipitation with KU-Lu-6 antibody.Lane 1: molecular weight marker, lane 2: LC-2/ad-cis lysate combined with KU-Lu-6 antibody, lane 3: LC-2/ad-cis lysate combined with protein L, lane 4: KU-Lu-6 antibody combined with protein L, lane 5: LC-2/ad-cis lysate. Lanes 3 and 4 are negative controls, and specific immunoprecipitated product with KU-Lu-6 antibody was detected in lane 2 (arrow).
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pone.0121460.g003: Immunoprecipitation with KU-Lu-6 antibody.Lane 1: molecular weight marker, lane 2: LC-2/ad-cis lysate combined with KU-Lu-6 antibody, lane 3: LC-2/ad-cis lysate combined with protein L, lane 4: KU-Lu-6 antibody combined with protein L, lane 5: LC-2/ad-cis lysate. Lanes 3 and 4 are negative controls, and specific immunoprecipitated product with KU-Lu-6 antibody was detected in lane 2 (arrow).

Mentions: In order to identify the antigen protein recognized by the KU-Lu-6 antibody, we performed IP with lysate from LC-2/ad-cis cells. The results of IP are shown in Fig. 2. The antigenic protein was observed at roughly 250 kDa in lane 2 (Fig. 3). No signal was observed in lane 3 and 4 as a negative control (Fig. 3). To determine the antigenic protein recognized by KU-Lu-6 antibody, we excised and collected the spot from the Zn-stained gel, and proceeded with in-gel digestion. After analysis employing a MALDI-TOF/TOF MS and MASCOT search, the protein was determined as isoform 1 of myosin-9 (MYH9, accession: IPI00019502), which is composed of 1,960 amino acids with a predicted M.W. of 226,532 Da. The immunoglobulin isotype of KU-Lu-6 antibody was determined as IgM, k. By the antibody absorption test, the stainability of KU-Lu-6 antibody was gradually reduced depending on the concentration of MYH9 protein. The stainability of KU-Lu-6 antibody was completely lost with 0.48 ug of MYH9 protein (Fig. 2).


Prognostic significance of MYH9 expression in resected non-small cell lung cancer.

Katono K, Sato Y, Jiang SX, Kobayashi M, Nagashio R, Ryuge S, Fukuda E, Goshima N, Satoh Y, Saegusa M, Masuda N - PLoS ONE (2015)

Immunoprecipitation with KU-Lu-6 antibody.Lane 1: molecular weight marker, lane 2: LC-2/ad-cis lysate combined with KU-Lu-6 antibody, lane 3: LC-2/ad-cis lysate combined with protein L, lane 4: KU-Lu-6 antibody combined with protein L, lane 5: LC-2/ad-cis lysate. Lanes 3 and 4 are negative controls, and specific immunoprecipitated product with KU-Lu-6 antibody was detected in lane 2 (arrow).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380450&req=5

pone.0121460.g003: Immunoprecipitation with KU-Lu-6 antibody.Lane 1: molecular weight marker, lane 2: LC-2/ad-cis lysate combined with KU-Lu-6 antibody, lane 3: LC-2/ad-cis lysate combined with protein L, lane 4: KU-Lu-6 antibody combined with protein L, lane 5: LC-2/ad-cis lysate. Lanes 3 and 4 are negative controls, and specific immunoprecipitated product with KU-Lu-6 antibody was detected in lane 2 (arrow).
Mentions: In order to identify the antigen protein recognized by the KU-Lu-6 antibody, we performed IP with lysate from LC-2/ad-cis cells. The results of IP are shown in Fig. 2. The antigenic protein was observed at roughly 250 kDa in lane 2 (Fig. 3). No signal was observed in lane 3 and 4 as a negative control (Fig. 3). To determine the antigenic protein recognized by KU-Lu-6 antibody, we excised and collected the spot from the Zn-stained gel, and proceeded with in-gel digestion. After analysis employing a MALDI-TOF/TOF MS and MASCOT search, the protein was determined as isoform 1 of myosin-9 (MYH9, accession: IPI00019502), which is composed of 1,960 amino acids with a predicted M.W. of 226,532 Da. The immunoglobulin isotype of KU-Lu-6 antibody was determined as IgM, k. By the antibody absorption test, the stainability of KU-Lu-6 antibody was gradually reduced depending on the concentration of MYH9 protein. The stainability of KU-Lu-6 antibody was completely lost with 0.48 ug of MYH9 protein (Fig. 2).

Bottom Line: Recently, MYH9 has been thought to be associated with cancer cell migration, invasion, and metastasis.Kaplan-Meier survival analysis and Cox proportional hazards models were used to estimate the effect of MYH9 expression on survival.MYH9 expression was significantly correlated with the adenocarcinoma histology (P = 0.014), poorer differentiation ((P = 0.033), intratumoral vascular invasion and lymphatic invasion ((P = 0.013 and P = 0.045 respectively), and a poorer prognosis ((P = 0.032).

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, School of Medicine, Kitasato University, Kanagawa, Japan.

ABSTRACT

Introduction: Myosin-9 (MYH9) belongs to the myosin superfamily of actin-binding motor protein. Recently, MYH9 has been thought to be associated with cancer cell migration, invasion, and metastasis. The aims of this study were to immunohistochemically examine MYH9 expression in surgically resected non-small cell lung cancer (NSCLC), and evaluate its correlations with clinicopathological parameters and the prognosis of patients.

Methods: MYH9 expression was immunohistochemically studied in 266 consecutive resected NSCLCs, and its associations with clinicopathological parameters were evaluated. Kaplan-Meier survival analysis and Cox proportional hazards models were used to estimate the effect of MYH9 expression on survival.

Results: MYH9 expression was detected in 102 of 266 (38.3%) NSCLCs. MYH9 expression was significantly correlated with the adenocarcinoma histology (P = 0.014), poorer differentiation ((P = 0.033), intratumoral vascular invasion and lymphatic invasion ((P = 0.013 and P = 0.045 respectively), and a poorer prognosis ((P = 0.032). In addition, multivariable analysis revealed that MYH9 expression independently predicted a poorer survival (HR, 2.15; 95%CI, 1.17-3.92; (P = 0.01).

Conclusion: The present study revealed that MYH9 is expressed in a subset of NSCLC with a more malignant nature, and its expression is an indicator of a poorer survival probability.

No MeSH data available.


Related in: MedlinePlus