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Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.

Gu Z, Imai F, Kim IJ, Fujita H, Katayama Ki, Mori K, Yoshihara Y, Yoshida Y - PLoS ONE (2015)

Bottom Line: Further analyses show that Ocam is expressed by obturator but not quadriceps motor neurons, suggesting that Ocam may regulate sensory-motor specificity in these sensory-motor reflex arcs.Since a subset of Ocam+ motor neurons also express Alcam, Alcam or other functionally redundant IgSF molecules may compensate for Ocam in controlling sensory-motor specificity.Taken together, these results reveal that IgSF molecules are broadly expressed by sensory and motor neurons during development, and that Ocam and other IgSF molecules may have redundant functions in controlling the specificity of sensory-motor circuits.

View Article: PubMed Central - PubMed

Affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.

ABSTRACT
Cell adhesion molecules belonging to the immunoglobulin superfamily (IgSF) control synaptic specificity through hetero- or homophilic interactions in different regions of the nervous system. In the developing spinal cord, monosynaptic connections of exquisite specificity form between proprioceptive sensory neurons and motor neurons, however, it is not known whether IgSF molecules participate in regulating this process. To determine whether IgSF molecules influence the establishment of synaptic specificity in sensory-motor circuits, we examined the expression of 157 IgSF genes in the developing dorsal root ganglion (DRG) and spinal cord by in situ hybridization assays. We find that many IgSF genes are expressed by sensory and motor neurons in the mouse developing DRG and spinal cord. For instance, Alcam, Mcam, and Ocam are expressed by a subset of motor neurons in the ventral spinal cord. Further analyses show that Ocam is expressed by obturator but not quadriceps motor neurons, suggesting that Ocam may regulate sensory-motor specificity in these sensory-motor reflex arcs. Electrophysiological analysis shows no obvious defects in synaptic specificity of monosynaptic sensory-motor connections involving obturator and quadriceps motor neurons in Ocam mutant mice. Since a subset of Ocam+ motor neurons also express Alcam, Alcam or other functionally redundant IgSF molecules may compensate for Ocam in controlling sensory-motor specificity. Taken together, these results reveal that IgSF molecules are broadly expressed by sensory and motor neurons during development, and that Ocam and other IgSF molecules may have redundant functions in controlling the specificity of sensory-motor circuits.

No MeSH data available.


Related in: MedlinePlus

Expression of Mcam in the developing DRG and spinal cord.(A-I) In situ hybridizations for Mcam on lumbar spinal cord sections from E16.5 (A-C), P0 (D-F), and P4 (G-I) wild-type mice. Mcam was strongly expressed by a subset of sensory and motor neurons at all stages. Expression of Mcam in the white matter was detected at P0 and P4 (D, E, G, and H).
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pone.0121550.g004: Expression of Mcam in the developing DRG and spinal cord.(A-I) In situ hybridizations for Mcam on lumbar spinal cord sections from E16.5 (A-C), P0 (D-F), and P4 (G-I) wild-type mice. Mcam was strongly expressed by a subset of sensory and motor neurons at all stages. Expression of Mcam in the white matter was detected at P0 and P4 (D, E, G, and H).

Mentions: We performed in situ hybridization assays for Mcam mRNA expression in the mouse DRG and spinal cord, and observed strong expression of Mcam in a subset of motor neurons at E16.5, P0, and P4 (Fig. 4A-4B, 4D-4E, and 4G-4H). In the DRG, Mcam was expressed by a subset of sensory neurons (Fig. 4C, 4F, and 4I). Since strong expression of Mcam in motor neurons was even detected at P0 and P4, Mcam may also be involved in synaptogenesis and the maintenance of sensory-motor connections.


Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.

Gu Z, Imai F, Kim IJ, Fujita H, Katayama Ki, Mori K, Yoshihara Y, Yoshida Y - PLoS ONE (2015)

Expression of Mcam in the developing DRG and spinal cord.(A-I) In situ hybridizations for Mcam on lumbar spinal cord sections from E16.5 (A-C), P0 (D-F), and P4 (G-I) wild-type mice. Mcam was strongly expressed by a subset of sensory and motor neurons at all stages. Expression of Mcam in the white matter was detected at P0 and P4 (D, E, G, and H).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4380438&req=5

pone.0121550.g004: Expression of Mcam in the developing DRG and spinal cord.(A-I) In situ hybridizations for Mcam on lumbar spinal cord sections from E16.5 (A-C), P0 (D-F), and P4 (G-I) wild-type mice. Mcam was strongly expressed by a subset of sensory and motor neurons at all stages. Expression of Mcam in the white matter was detected at P0 and P4 (D, E, G, and H).
Mentions: We performed in situ hybridization assays for Mcam mRNA expression in the mouse DRG and spinal cord, and observed strong expression of Mcam in a subset of motor neurons at E16.5, P0, and P4 (Fig. 4A-4B, 4D-4E, and 4G-4H). In the DRG, Mcam was expressed by a subset of sensory neurons (Fig. 4C, 4F, and 4I). Since strong expression of Mcam in motor neurons was even detected at P0 and P4, Mcam may also be involved in synaptogenesis and the maintenance of sensory-motor connections.

Bottom Line: Further analyses show that Ocam is expressed by obturator but not quadriceps motor neurons, suggesting that Ocam may regulate sensory-motor specificity in these sensory-motor reflex arcs.Since a subset of Ocam+ motor neurons also express Alcam, Alcam or other functionally redundant IgSF molecules may compensate for Ocam in controlling sensory-motor specificity.Taken together, these results reveal that IgSF molecules are broadly expressed by sensory and motor neurons during development, and that Ocam and other IgSF molecules may have redundant functions in controlling the specificity of sensory-motor circuits.

View Article: PubMed Central - PubMed

Affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.

ABSTRACT
Cell adhesion molecules belonging to the immunoglobulin superfamily (IgSF) control synaptic specificity through hetero- or homophilic interactions in different regions of the nervous system. In the developing spinal cord, monosynaptic connections of exquisite specificity form between proprioceptive sensory neurons and motor neurons, however, it is not known whether IgSF molecules participate in regulating this process. To determine whether IgSF molecules influence the establishment of synaptic specificity in sensory-motor circuits, we examined the expression of 157 IgSF genes in the developing dorsal root ganglion (DRG) and spinal cord by in situ hybridization assays. We find that many IgSF genes are expressed by sensory and motor neurons in the mouse developing DRG and spinal cord. For instance, Alcam, Mcam, and Ocam are expressed by a subset of motor neurons in the ventral spinal cord. Further analyses show that Ocam is expressed by obturator but not quadriceps motor neurons, suggesting that Ocam may regulate sensory-motor specificity in these sensory-motor reflex arcs. Electrophysiological analysis shows no obvious defects in synaptic specificity of monosynaptic sensory-motor connections involving obturator and quadriceps motor neurons in Ocam mutant mice. Since a subset of Ocam+ motor neurons also express Alcam, Alcam or other functionally redundant IgSF molecules may compensate for Ocam in controlling sensory-motor specificity. Taken together, these results reveal that IgSF molecules are broadly expressed by sensory and motor neurons during development, and that Ocam and other IgSF molecules may have redundant functions in controlling the specificity of sensory-motor circuits.

No MeSH data available.


Related in: MedlinePlus