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The impact of host diet on Wolbachia titer in Drosophila.

Serbus LR, White PM, Silva JP, Rabe A, Teixeira L, Albertson R, Sullivan W - PLoS Pathog. (2015)

Bottom Line: Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer.Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system.These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America; Biomolecular Sciences Institute, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America.

ABSTRACT
While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

No MeSH data available.


Related in: MedlinePlus

Nutrients affect germline Wolbachia titer through the somatic insulin pathway.Dietary impact on oocyte Wolbachia titer was tested in flies that either carried or lacked functional IPCs in the brain. Wild-type flies were A) treated with DMSO or B) induced with Mifepristone over a 14-day period as a control. {dilp2:GS-GAL4}; {UAS-rpr} flies were also C) treated with DMSO as a control, or D) induced with Mifepristone over a 14-day period to drive IPC lethality. * indicates significant changes in titer.
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ppat.1004777.g005: Nutrients affect germline Wolbachia titer through the somatic insulin pathway.Dietary impact on oocyte Wolbachia titer was tested in flies that either carried or lacked functional IPCs in the brain. Wild-type flies were A) treated with DMSO or B) induced with Mifepristone over a 14-day period as a control. {dilp2:GS-GAL4}; {UAS-rpr} flies were also C) treated with DMSO as a control, or D) induced with Mifepristone over a 14-day period to drive IPC lethality. * indicates significant changes in titer.

Mentions: We first investigated whether mifepristone on its own modulates the yeast effect in wild-type flies. After completing a two-week exposure to either DMSO or mifepristone, flies were exposed to either control or yeast-enriched food for 3 days, and their oocyte titer levels were assessed. DMSO-treated flies exhibited substantial oocyte titer depletion in response to yeast-enriched food, down to 30% of the titer in the control condition (Fig. 5A). This was indicated by 785 +/- 64.8 Wolbachia per oocyte in the DMSO-control food condition (n = 24), in contrast to 191 +/- 26.9 Wolbachia in the DMSO-yeast-enriched condition (n = 25) (p <. 001) (Fig. 5A). Mifepristone-treated flies showed a similar titer reduction after exposure to yeast, exhibiting 21% of the titer seen in the control food condition (Fig. 5B). This was indicated by 896 +/- 77.2 Wolbachia per oocyte in the mifepristone-control food condition (n = 23), versus 264 +/- 39.5 Wolbachia in the mifepristone-yeast-enriched condition (n = 25) (Fig. 5B) (p <. 001). Therefore, mifepristone alone has no effect on yeast-based suppression of oocyte Wolbachia titer.


The impact of host diet on Wolbachia titer in Drosophila.

Serbus LR, White PM, Silva JP, Rabe A, Teixeira L, Albertson R, Sullivan W - PLoS Pathog. (2015)

Nutrients affect germline Wolbachia titer through the somatic insulin pathway.Dietary impact on oocyte Wolbachia titer was tested in flies that either carried or lacked functional IPCs in the brain. Wild-type flies were A) treated with DMSO or B) induced with Mifepristone over a 14-day period as a control. {dilp2:GS-GAL4}; {UAS-rpr} flies were also C) treated with DMSO as a control, or D) induced with Mifepristone over a 14-day period to drive IPC lethality. * indicates significant changes in titer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380406&req=5

ppat.1004777.g005: Nutrients affect germline Wolbachia titer through the somatic insulin pathway.Dietary impact on oocyte Wolbachia titer was tested in flies that either carried or lacked functional IPCs in the brain. Wild-type flies were A) treated with DMSO or B) induced with Mifepristone over a 14-day period as a control. {dilp2:GS-GAL4}; {UAS-rpr} flies were also C) treated with DMSO as a control, or D) induced with Mifepristone over a 14-day period to drive IPC lethality. * indicates significant changes in titer.
Mentions: We first investigated whether mifepristone on its own modulates the yeast effect in wild-type flies. After completing a two-week exposure to either DMSO or mifepristone, flies were exposed to either control or yeast-enriched food for 3 days, and their oocyte titer levels were assessed. DMSO-treated flies exhibited substantial oocyte titer depletion in response to yeast-enriched food, down to 30% of the titer in the control condition (Fig. 5A). This was indicated by 785 +/- 64.8 Wolbachia per oocyte in the DMSO-control food condition (n = 24), in contrast to 191 +/- 26.9 Wolbachia in the DMSO-yeast-enriched condition (n = 25) (p <. 001) (Fig. 5A). Mifepristone-treated flies showed a similar titer reduction after exposure to yeast, exhibiting 21% of the titer seen in the control food condition (Fig. 5B). This was indicated by 896 +/- 77.2 Wolbachia per oocyte in the mifepristone-control food condition (n = 23), versus 264 +/- 39.5 Wolbachia in the mifepristone-yeast-enriched condition (n = 25) (Fig. 5B) (p <. 001). Therefore, mifepristone alone has no effect on yeast-based suppression of oocyte Wolbachia titer.

Bottom Line: Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer.Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system.These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America; Biomolecular Sciences Institute, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America.

ABSTRACT
While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

No MeSH data available.


Related in: MedlinePlus