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The impact of host diet on Wolbachia titer in Drosophila.

Serbus LR, White PM, Silva JP, Rabe A, Teixeira L, Albertson R, Sullivan W - PLoS Pathog. (2015)

Bottom Line: Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer.Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system.These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America; Biomolecular Sciences Institute, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America.

ABSTRACT
While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

No MeSH data available.


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Overview of the nutrient-induced TORC1 signaling pathway.
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ppat.1004777.g001: Overview of the nutrient-induced TORC1 signaling pathway.

Mentions: Here we examined how host diet affects Wolbachia titer in Drosophila melanogaster. The data demonstrate that yeast-enriched diets suppress Wolbachia titer and lead to altered nucleoid morphology during oogenesis. Genetic and chemical disruptions indicate that the somatic insulin and TORC1 pathways (Fig. 1) are required for yeast-based suppression of oocyte Wolbachia titer. The data also indicate that sucrose-enriched diets increased oocyte Wolbachia titer, with little impact on nucleoid morphology. Evidence indicates that yeast-enriched diets substantially increase somatic Wolbachia titers, though this was not the case in the central nervous system (CNS). These studies demonstrate that Wolbachia, and likely other bacterial endosymbionts, exhibit distinct, tissue-specific responses to host nutrients that involve conserved signaling and metabolic pathways.


The impact of host diet on Wolbachia titer in Drosophila.

Serbus LR, White PM, Silva JP, Rabe A, Teixeira L, Albertson R, Sullivan W - PLoS Pathog. (2015)

Overview of the nutrient-induced TORC1 signaling pathway.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380406&req=5

ppat.1004777.g001: Overview of the nutrient-induced TORC1 signaling pathway.
Mentions: Here we examined how host diet affects Wolbachia titer in Drosophila melanogaster. The data demonstrate that yeast-enriched diets suppress Wolbachia titer and lead to altered nucleoid morphology during oogenesis. Genetic and chemical disruptions indicate that the somatic insulin and TORC1 pathways (Fig. 1) are required for yeast-based suppression of oocyte Wolbachia titer. The data also indicate that sucrose-enriched diets increased oocyte Wolbachia titer, with little impact on nucleoid morphology. Evidence indicates that yeast-enriched diets substantially increase somatic Wolbachia titers, though this was not the case in the central nervous system (CNS). These studies demonstrate that Wolbachia, and likely other bacterial endosymbionts, exhibit distinct, tissue-specific responses to host nutrients that involve conserved signaling and metabolic pathways.

Bottom Line: Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer.Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system.These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America; Biomolecular Sciences Institute, Florida International University Modesto A. Maidique Campus, Miami, Florida, United States of America.

ABSTRACT
While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

No MeSH data available.


Related in: MedlinePlus