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Long non-coding RNA expression profile in the kidneys of male, low birth weight rats exposed to maternal protein restriction at postnatal day 1 and day 10.

Li Y, Wang X, Li M, Pan J, Jin M, Wang J, Li X, Feng X - PLoS ONE (2015)

Bottom Line: Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups.According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Children's Hospital of Soochow University, Suzhou, China; Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, China.

ABSTRACT

Background: Long non-coding RNAs (lncRNAs), which are involved in a variety of biological functions and aberrantly expressed in many types of diseases, are required for postnatal development. In this study, we aimed to investigate the lncRNA profiles in low birth weight (LBW) rats with reduced nephron endowment induced by the restriction of maternal protein intake. LBW by reduced nephron endowment is a risk factor for hypertension and end-stage renal disease in adulthood.

Methods: Kidneys were obtained from LBW rats fed a low-protein diet throughout gestation and lactation as well as from normal control rats born from dams fed normal protein diets at postnatal day 1 (p1) and 10 (p10). The total number of glomeruli in the kidneys was counted at p10. LncRNA expression profiles were analyzed by sequencing and screening using the Agilent Rat lncRNA Array. Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.

Results: The total number of glomeruli per kidney at p10 was significantly lower in LBW rats than in controls. A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups. According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR. Three lncRNAs (TCONS_00014139, TCONS_00014138, and TCONS_00017119), which were significantly correlated with the mRNA expression of mitogen-activated protein kinase 4, were aberrantly expressed in LBW rats, compared with controls, at both p1 and p10.

Conclusions: LncRNAs are aberrantly expressed in the kidneys of LBW rats, compared with controls, during nephron development, which indicates that lncRNAs might be involved in impaired nephron endowment.

No MeSH data available.


Related in: MedlinePlus

Comparison of lncRNA expression in different tissues.Comparison of the expression of lncRNA TCONS_00017119 in different tissues from low birth weight and control rats at postnatal day 10 by quantitative real-time PCR. The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to lncRNA level in the brain of normal control rats. Values are means with SEM. p Value was calculated by the Student’s t-test. Black bars, control rats (n = 5); open bars, low birth weight rats (n = 5). *p <0.05 vs. low birth weight rats, #p <0.05 vs. normal kidney.
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pone.0121587.g006: Comparison of lncRNA expression in different tissues.Comparison of the expression of lncRNA TCONS_00017119 in different tissues from low birth weight and control rats at postnatal day 10 by quantitative real-time PCR. The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to lncRNA level in the brain of normal control rats. Values are means with SEM. p Value was calculated by the Student’s t-test. Black bars, control rats (n = 5); open bars, low birth weight rats (n = 5). *p <0.05 vs. low birth weight rats, #p <0.05 vs. normal kidney.

Mentions: To determine whether the differential expression of lncRNAs was specific to the kidneys, we examined the expression level of these three lncRNAs in kidney, liver, brain, and lung tissues, in a separate experiment, from LBW and normal control rats at p10 using qRT-PCR (n = 5 for each group). The expression of lncRNA TCONS_0014139 was negligible in the rat tissues of liver, brain, and lung, suggesting that TCONS_0014139 expression might be specific to the kidneys. LncRNA TCONS_00014138 was detectable in normal liver and lung. However, the expression of TCONS_00014138 in LBW rat tissues of liver (p = 0.987) and lung (p = 0.212) did not significantly differ from that in controls at p10. LncRNA TCONS_00017119 was detected in rat tissues of liver, brain, and lung, and expressed at high levels in normal liver and lung. As shown in Fig 6, the expression of TCONS_00017119 was higher in normal liver than in kidney (p = 0.010). There was a significant decrease in the expression of TCONS_00017119 in liver of LBW rats compared with normal controls at p10 (p = 0.009). There was no significant difference in the expression of TCONS_00017119 in normal lung compared with normal kidney (p = 0.092). There was also no difference in TCONS_00017119 expression in lung of LBW rats compared with controls (p = 0.896). These results indicate that the changes of these potential lncRNAs expression in LBW rats might be specific to the kidneys.


Long non-coding RNA expression profile in the kidneys of male, low birth weight rats exposed to maternal protein restriction at postnatal day 1 and day 10.

Li Y, Wang X, Li M, Pan J, Jin M, Wang J, Li X, Feng X - PLoS ONE (2015)

Comparison of lncRNA expression in different tissues.Comparison of the expression of lncRNA TCONS_00017119 in different tissues from low birth weight and control rats at postnatal day 10 by quantitative real-time PCR. The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to lncRNA level in the brain of normal control rats. Values are means with SEM. p Value was calculated by the Student’s t-test. Black bars, control rats (n = 5); open bars, low birth weight rats (n = 5). *p <0.05 vs. low birth weight rats, #p <0.05 vs. normal kidney.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4380357&req=5

pone.0121587.g006: Comparison of lncRNA expression in different tissues.Comparison of the expression of lncRNA TCONS_00017119 in different tissues from low birth weight and control rats at postnatal day 10 by quantitative real-time PCR. The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to lncRNA level in the brain of normal control rats. Values are means with SEM. p Value was calculated by the Student’s t-test. Black bars, control rats (n = 5); open bars, low birth weight rats (n = 5). *p <0.05 vs. low birth weight rats, #p <0.05 vs. normal kidney.
Mentions: To determine whether the differential expression of lncRNAs was specific to the kidneys, we examined the expression level of these three lncRNAs in kidney, liver, brain, and lung tissues, in a separate experiment, from LBW and normal control rats at p10 using qRT-PCR (n = 5 for each group). The expression of lncRNA TCONS_0014139 was negligible in the rat tissues of liver, brain, and lung, suggesting that TCONS_0014139 expression might be specific to the kidneys. LncRNA TCONS_00014138 was detectable in normal liver and lung. However, the expression of TCONS_00014138 in LBW rat tissues of liver (p = 0.987) and lung (p = 0.212) did not significantly differ from that in controls at p10. LncRNA TCONS_00017119 was detected in rat tissues of liver, brain, and lung, and expressed at high levels in normal liver and lung. As shown in Fig 6, the expression of TCONS_00017119 was higher in normal liver than in kidney (p = 0.010). There was a significant decrease in the expression of TCONS_00017119 in liver of LBW rats compared with normal controls at p10 (p = 0.009). There was no significant difference in the expression of TCONS_00017119 in normal lung compared with normal kidney (p = 0.092). There was also no difference in TCONS_00017119 expression in lung of LBW rats compared with controls (p = 0.896). These results indicate that the changes of these potential lncRNAs expression in LBW rats might be specific to the kidneys.

Bottom Line: Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups.According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Children's Hospital of Soochow University, Suzhou, China; Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, China.

ABSTRACT

Background: Long non-coding RNAs (lncRNAs), which are involved in a variety of biological functions and aberrantly expressed in many types of diseases, are required for postnatal development. In this study, we aimed to investigate the lncRNA profiles in low birth weight (LBW) rats with reduced nephron endowment induced by the restriction of maternal protein intake. LBW by reduced nephron endowment is a risk factor for hypertension and end-stage renal disease in adulthood.

Methods: Kidneys were obtained from LBW rats fed a low-protein diet throughout gestation and lactation as well as from normal control rats born from dams fed normal protein diets at postnatal day 1 (p1) and 10 (p10). The total number of glomeruli in the kidneys was counted at p10. LncRNA expression profiles were analyzed by sequencing and screening using the Agilent Rat lncRNA Array. Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.

Results: The total number of glomeruli per kidney at p10 was significantly lower in LBW rats than in controls. A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups. According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR. Three lncRNAs (TCONS_00014139, TCONS_00014138, and TCONS_00017119), which were significantly correlated with the mRNA expression of mitogen-activated protein kinase 4, were aberrantly expressed in LBW rats, compared with controls, at both p1 and p10.

Conclusions: LncRNAs are aberrantly expressed in the kidneys of LBW rats, compared with controls, during nephron development, which indicates that lncRNAs might be involved in impaired nephron endowment.

No MeSH data available.


Related in: MedlinePlus