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Long non-coding RNA expression profile in the kidneys of male, low birth weight rats exposed to maternal protein restriction at postnatal day 1 and day 10.

Li Y, Wang X, Li M, Pan J, Jin M, Wang J, Li X, Feng X - PLoS ONE (2015)

Bottom Line: Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups.According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Children's Hospital of Soochow University, Suzhou, China; Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, China.

ABSTRACT

Background: Long non-coding RNAs (lncRNAs), which are involved in a variety of biological functions and aberrantly expressed in many types of diseases, are required for postnatal development. In this study, we aimed to investigate the lncRNA profiles in low birth weight (LBW) rats with reduced nephron endowment induced by the restriction of maternal protein intake. LBW by reduced nephron endowment is a risk factor for hypertension and end-stage renal disease in adulthood.

Methods: Kidneys were obtained from LBW rats fed a low-protein diet throughout gestation and lactation as well as from normal control rats born from dams fed normal protein diets at postnatal day 1 (p1) and 10 (p10). The total number of glomeruli in the kidneys was counted at p10. LncRNA expression profiles were analyzed by sequencing and screening using the Agilent Rat lncRNA Array. Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.

Results: The total number of glomeruli per kidney at p10 was significantly lower in LBW rats than in controls. A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups. According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR. Three lncRNAs (TCONS_00014139, TCONS_00014138, and TCONS_00017119), which were significantly correlated with the mRNA expression of mitogen-activated protein kinase 4, were aberrantly expressed in LBW rats, compared with controls, at both p1 and p10.

Conclusions: LncRNAs are aberrantly expressed in the kidneys of LBW rats, compared with controls, during nephron development, which indicates that lncRNAs might be involved in impaired nephron endowment.

No MeSH data available.


Related in: MedlinePlus

Correlation analysis of lncRNAs expression with glomerular number.The number of glomeruli was positively correlated with the relative expression of lncRNAs TCONS_0014139 (A: r = 0.88; p <0.001; n = 14) and TCONS_00014138 (B: r = 0.77; p = 0.001; n = 14), but negatively with lncRNA TCONS_00017119 (C: r = -0.80; p = 0.001; n = 14) in the kidneys at postnatal day 10 (p10). Black circles, control rats (n = 7); open circles, low birth weight rats (n = 7). The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to the controls at p10. r = Spearman’s correlation coefficient.
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pone.0121587.g005: Correlation analysis of lncRNAs expression with glomerular number.The number of glomeruli was positively correlated with the relative expression of lncRNAs TCONS_0014139 (A: r = 0.88; p <0.001; n = 14) and TCONS_00014138 (B: r = 0.77; p = 0.001; n = 14), but negatively with lncRNA TCONS_00017119 (C: r = -0.80; p = 0.001; n = 14) in the kidneys at postnatal day 10 (p10). Black circles, control rats (n = 7); open circles, low birth weight rats (n = 7). The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to the controls at p10. r = Spearman’s correlation coefficient.

Mentions: The correlations between these three lncRNAs and glomerular number are shown in Fig 5. The total number of glomeruli per kidney was significantly positively correlated with the relative expression level of lncRNAs TCONS_0014139 and TCONS_00014138, but negatively with lncRNA TCONS_00017119, at p10.


Long non-coding RNA expression profile in the kidneys of male, low birth weight rats exposed to maternal protein restriction at postnatal day 1 and day 10.

Li Y, Wang X, Li M, Pan J, Jin M, Wang J, Li X, Feng X - PLoS ONE (2015)

Correlation analysis of lncRNAs expression with glomerular number.The number of glomeruli was positively correlated with the relative expression of lncRNAs TCONS_0014139 (A: r = 0.88; p <0.001; n = 14) and TCONS_00014138 (B: r = 0.77; p = 0.001; n = 14), but negatively with lncRNA TCONS_00017119 (C: r = -0.80; p = 0.001; n = 14) in the kidneys at postnatal day 10 (p10). Black circles, control rats (n = 7); open circles, low birth weight rats (n = 7). The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to the controls at p10. r = Spearman’s correlation coefficient.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380357&req=5

pone.0121587.g005: Correlation analysis of lncRNAs expression with glomerular number.The number of glomeruli was positively correlated with the relative expression of lncRNAs TCONS_0014139 (A: r = 0.88; p <0.001; n = 14) and TCONS_00014138 (B: r = 0.77; p = 0.001; n = 14), but negatively with lncRNA TCONS_00017119 (C: r = -0.80; p = 0.001; n = 14) in the kidneys at postnatal day 10 (p10). Black circles, control rats (n = 7); open circles, low birth weight rats (n = 7). The level of lncRNA was calculated relative to GAPDH. The data are expressed as relative to the controls at p10. r = Spearman’s correlation coefficient.
Mentions: The correlations between these three lncRNAs and glomerular number are shown in Fig 5. The total number of glomeruli per kidney was significantly positively correlated with the relative expression level of lncRNAs TCONS_0014139 and TCONS_00014138, but negatively with lncRNA TCONS_00017119, at p10.

Bottom Line: Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups.According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Children's Hospital of Soochow University, Suzhou, China; Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, China.

ABSTRACT

Background: Long non-coding RNAs (lncRNAs), which are involved in a variety of biological functions and aberrantly expressed in many types of diseases, are required for postnatal development. In this study, we aimed to investigate the lncRNA profiles in low birth weight (LBW) rats with reduced nephron endowment induced by the restriction of maternal protein intake. LBW by reduced nephron endowment is a risk factor for hypertension and end-stage renal disease in adulthood.

Methods: Kidneys were obtained from LBW rats fed a low-protein diet throughout gestation and lactation as well as from normal control rats born from dams fed normal protein diets at postnatal day 1 (p1) and 10 (p10). The total number of glomeruli in the kidneys was counted at p10. LncRNA expression profiles were analyzed by sequencing and screening using the Agilent Rat lncRNA Array. Quantitative real-time PCR (qRT-PCR) analysis of these lncRNAs confirmed the identity of some genes.

Results: The total number of glomeruli per kidney at p10 was significantly lower in LBW rats than in controls. A total of 42 lncRNAs were identified to be significantly differentially expressed, with fold-changes ≥2.0, between the two groups. According to correlation analysis between the differentially expressed lncRNAs and mRNAs involved in kidney development, we randomly selected a number of lncRNAs for comparison analysis between LBW and control kidneys at the two time-points, p1 and p10, using qRT-PCR. Three lncRNAs (TCONS_00014139, TCONS_00014138, and TCONS_00017119), which were significantly correlated with the mRNA expression of mitogen-activated protein kinase 4, were aberrantly expressed in LBW rats, compared with controls, at both p1 and p10.

Conclusions: LncRNAs are aberrantly expressed in the kidneys of LBW rats, compared with controls, during nephron development, which indicates that lncRNAs might be involved in impaired nephron endowment.

No MeSH data available.


Related in: MedlinePlus