Limits...
Brain isoform glycogen phosphorylase as a novel hepatic progenitor cell marker.

Huang YW, Chiu CC, Liang JD, Chiou LL, Huang GT, Yu MJ, Lee HS - PLoS ONE (2015)

Bottom Line: Immunoblotting results show that GPBB was expressed in two liver progenitor cell lines Lig-8 and WB-F344.Short hairpin RNA (shRNA)-mediated GPBB knockdown followed by glucose deprivation test shows that GPBB aids in liver progenitor cell survival under low glucose conditions.Furthermore, shRNA-mediated GPBB knockdown followed by SB-induced cell differentiation shows that reducing GPBB expression delayed liver progenitor cell differentiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan; Division of Medical Devices and Cosmetics, Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan.

ABSTRACT
An appropriate liver-specific progenitor cell marker is a stepping stone in liver regenerative medicine. Here, we report brain isoform glycogen phosphorylase (GPBB) as a novel liver progenitor cell marker. GPBB was identified in a protein complex precipitated by a monoclonal antibody Ligab generated from a rat liver progenitor cell line Lig-8. Immunoblotting results show that GPBB was expressed in two liver progenitor cell lines Lig-8 and WB-F344. The levels of GPBB expression decreased in the WB-F344 cells under sodium butyrate (SB)-induced cell differentiation, consistent with roles of GPBB as a liver progenitor cell marker. Short hairpin RNA (shRNA)-mediated GPBB knockdown followed by glucose deprivation test shows that GPBB aids in liver progenitor cell survival under low glucose conditions. Furthermore, shRNA-mediated GPBB knockdown followed by SB-induced cell differentiation shows that reducing GPBB expression delayed liver progenitor cell differentiation. We conclude that GPBB is a novel liver progenitor cell marker, which facilitates liver progenitor cell survival under low glucose conditions and cell differentiation.

No MeSH data available.


Related in: MedlinePlus

Time course expression of GPBB, GPLL and CK19 in WB-F344 cells during sodium butyrate (SB)-induced differentiation.(A) shows representative immunoblotting results and (B) shows a summary of 3 independent immunoblotting results. The cells were plated onto dishes on day 0 and SB was added into culture conditions on day 1. Y-axis: quantity, intensity of interest protein band over that of β-actin. Values are mean ± SEM. * P < 0.05, Student’s test against values of the day 1.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4380311&req=5

pone.0122528.g006: Time course expression of GPBB, GPLL and CK19 in WB-F344 cells during sodium butyrate (SB)-induced differentiation.(A) shows representative immunoblotting results and (B) shows a summary of 3 independent immunoblotting results. The cells were plated onto dishes on day 0 and SB was added into culture conditions on day 1. Y-axis: quantity, intensity of interest protein band over that of β-actin. Values are mean ± SEM. * P < 0.05, Student’s test against values of the day 1.

Mentions: To verify GPBB as a potential liver stem cell marker, sodium butyrate was used to induce WB-F344 cell differentiation followed by examining levels of GPBB protein along with mature cell makers CK19 and GPLL with immunoblotting. Upon sodium butyrate addition to the WB-F344 cells, the mature cell markers CK19 and GPLL were detected on day 3 and continuously increased on day 5 (Fig. 6), indicative of cell differentiation. During the cell differentiation process, GPBB protein levels decreased in a time-dependent manner to about 71% on day 3 and 56% on day 5 compared to those on day 1.


Brain isoform glycogen phosphorylase as a novel hepatic progenitor cell marker.

Huang YW, Chiu CC, Liang JD, Chiou LL, Huang GT, Yu MJ, Lee HS - PLoS ONE (2015)

Time course expression of GPBB, GPLL and CK19 in WB-F344 cells during sodium butyrate (SB)-induced differentiation.(A) shows representative immunoblotting results and (B) shows a summary of 3 independent immunoblotting results. The cells were plated onto dishes on day 0 and SB was added into culture conditions on day 1. Y-axis: quantity, intensity of interest protein band over that of β-actin. Values are mean ± SEM. * P < 0.05, Student’s test against values of the day 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380311&req=5

pone.0122528.g006: Time course expression of GPBB, GPLL and CK19 in WB-F344 cells during sodium butyrate (SB)-induced differentiation.(A) shows representative immunoblotting results and (B) shows a summary of 3 independent immunoblotting results. The cells were plated onto dishes on day 0 and SB was added into culture conditions on day 1. Y-axis: quantity, intensity of interest protein band over that of β-actin. Values are mean ± SEM. * P < 0.05, Student’s test against values of the day 1.
Mentions: To verify GPBB as a potential liver stem cell marker, sodium butyrate was used to induce WB-F344 cell differentiation followed by examining levels of GPBB protein along with mature cell makers CK19 and GPLL with immunoblotting. Upon sodium butyrate addition to the WB-F344 cells, the mature cell markers CK19 and GPLL were detected on day 3 and continuously increased on day 5 (Fig. 6), indicative of cell differentiation. During the cell differentiation process, GPBB protein levels decreased in a time-dependent manner to about 71% on day 3 and 56% on day 5 compared to those on day 1.

Bottom Line: Immunoblotting results show that GPBB was expressed in two liver progenitor cell lines Lig-8 and WB-F344.Short hairpin RNA (shRNA)-mediated GPBB knockdown followed by glucose deprivation test shows that GPBB aids in liver progenitor cell survival under low glucose conditions.Furthermore, shRNA-mediated GPBB knockdown followed by SB-induced cell differentiation shows that reducing GPBB expression delayed liver progenitor cell differentiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan; Division of Medical Devices and Cosmetics, Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan.

ABSTRACT
An appropriate liver-specific progenitor cell marker is a stepping stone in liver regenerative medicine. Here, we report brain isoform glycogen phosphorylase (GPBB) as a novel liver progenitor cell marker. GPBB was identified in a protein complex precipitated by a monoclonal antibody Ligab generated from a rat liver progenitor cell line Lig-8. Immunoblotting results show that GPBB was expressed in two liver progenitor cell lines Lig-8 and WB-F344. The levels of GPBB expression decreased in the WB-F344 cells under sodium butyrate (SB)-induced cell differentiation, consistent with roles of GPBB as a liver progenitor cell marker. Short hairpin RNA (shRNA)-mediated GPBB knockdown followed by glucose deprivation test shows that GPBB aids in liver progenitor cell survival under low glucose conditions. Furthermore, shRNA-mediated GPBB knockdown followed by SB-induced cell differentiation shows that reducing GPBB expression delayed liver progenitor cell differentiation. We conclude that GPBB is a novel liver progenitor cell marker, which facilitates liver progenitor cell survival under low glucose conditions and cell differentiation.

No MeSH data available.


Related in: MedlinePlus