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Genome and infection characteristics of human parechovirus type 1: the interplay between viral infection and type I interferon antiviral system.

Chang JT, Yang CS, Chen YS, Chen BC, Chiang AJ, Chang YH, Tsai WL, Lin YS, Chao D, Chang TH - PLoS ONE (2015)

Bottom Line: Human parechoviruses (HPeVs), members of the family Picornaviridae, are associated with severe human clinical conditions such as gastrointestinal disease, encephalitis, meningitis, respiratory disease and neonatal sepsis.A facilitated cytopathic effect and increased viral titers were reached after serial viral passages in Vero cells, with viral genome mutation found in later passages.The characterization of the complete genome and infection features of HPeV1 provide comprehensive information about this newly isolated HPeV1 for further diagnosis, prevention or treatment strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan; Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; Department of Pediatrics; Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.

ABSTRACT
Human parechoviruses (HPeVs), members of the family Picornaviridae, are associated with severe human clinical conditions such as gastrointestinal disease, encephalitis, meningitis, respiratory disease and neonatal sepsis. A new contemporary strain of HPeV1, KVP6 (accession no. KC769584), was isolated from a clinical specimen. Full-genome alignment revealed that HPeV1 KVP6 shares high genome homology with the German strain of HPeV1, 7555312 (accession no. FM178558) and could be classified in the clade 1B group. An intertypic recombination was shown within the P2-P3 genome regions of HPeV1. Cell-type tropism test showed that T84 cells (colon carcinoma cells), A549 cells (lung carcinoma cells) and DBTRG-5MG cells (glioblastoma cells) were susceptible to HPeV1 infection, which might be relevant clinically. A facilitated cytopathic effect and increased viral titers were reached after serial viral passages in Vero cells, with viral genome mutation found in later passages. HPeV1 is sensitive to elevated temperature because 39C incubation impaired virion production. HPeV1 induced innate immunity with phosphorylation of interferon (IFN) regulatory transcription factor 3 and production of type I IFN in A549 but not T84 cells. Furthermore, type I IFN inhibited HPeV1 production in A549 cells but not T84 cells; T84 cells may be less responsive to type I IFN stimulation. Moreover, HPeV1-infected cells showed downregulated type I IFN activation, which indicated a type I IFN evasion mechanism. The characterization of the complete genome and infection features of HPeV1 provide comprehensive information about this newly isolated HPeV1 for further diagnosis, prevention or treatment strategies.

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Cell tropism of HPeV1.Vero, T84, A549, DBTRG-5MG, BHK21, HeLa and J774A.1 cells at2×105 were infected with HPeV1 for 6 h atmultiplicity of infection (MOI) = 1; HPeV1 VP0 was detected byimmunofluorescence assay with anti-VP0 antibody; images show the redfluorescence of VP0 staining in susceptible cell types. DAPI stainingindicated cell nucleus.
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pone.0116158.g002: Cell tropism of HPeV1.Vero, T84, A549, DBTRG-5MG, BHK21, HeLa and J774A.1 cells at2×105 were infected with HPeV1 for 6 h atmultiplicity of infection (MOI) = 1; HPeV1 VP0 was detected byimmunofluorescence assay with anti-VP0 antibody; images show the redfluorescence of VP0 staining in susceptible cell types. DAPI stainingindicated cell nucleus.

Mentions: To characterize the infection features of this newly isolated HPeV1, we testedthe cell type tropism of HPeV1; various cell types were infected with HPeV1, andtheir susceptibility to infection was measured by immunofluorescence assay withanti-HPeV1 VP0 antibody. Vero cells (monkey kidney epithelial cells) are widelyused in virology and show susceptibility for HPeV1 infection, which could be apositive control for infection. In addition, T84 cells (intestinal carcinoma),A549 cells (lung carcinoma cells) and DBTRG-5MG (glioblastoma cells) weresusceptible to HPeV infection; BHK21 cells (baby hamster kindney epithelialcells), HeLa cells (human cervival cancer cells) and J774A.1 cells (mousemacrophages) were not infected with HPeV1 (Fig. 2). Because HPeV1 infection causes clinicalsymptoms including respiratory- and gastrointestinal-tract and CNS symptoms[25], the celltropism results might reflect in part an association of HPeV1 infection and itstarget organs. Our data also support the cell tropism of HPeV1 Harris strain andother clinically isolated HPeV1 strains [31,37].


Genome and infection characteristics of human parechovirus type 1: the interplay between viral infection and type I interferon antiviral system.

Chang JT, Yang CS, Chen YS, Chen BC, Chiang AJ, Chang YH, Tsai WL, Lin YS, Chao D, Chang TH - PLoS ONE (2015)

Cell tropism of HPeV1.Vero, T84, A549, DBTRG-5MG, BHK21, HeLa and J774A.1 cells at2×105 were infected with HPeV1 for 6 h atmultiplicity of infection (MOI) = 1; HPeV1 VP0 was detected byimmunofluorescence assay with anti-VP0 antibody; images show the redfluorescence of VP0 staining in susceptible cell types. DAPI stainingindicated cell nucleus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380134&req=5

pone.0116158.g002: Cell tropism of HPeV1.Vero, T84, A549, DBTRG-5MG, BHK21, HeLa and J774A.1 cells at2×105 were infected with HPeV1 for 6 h atmultiplicity of infection (MOI) = 1; HPeV1 VP0 was detected byimmunofluorescence assay with anti-VP0 antibody; images show the redfluorescence of VP0 staining in susceptible cell types. DAPI stainingindicated cell nucleus.
Mentions: To characterize the infection features of this newly isolated HPeV1, we testedthe cell type tropism of HPeV1; various cell types were infected with HPeV1, andtheir susceptibility to infection was measured by immunofluorescence assay withanti-HPeV1 VP0 antibody. Vero cells (monkey kidney epithelial cells) are widelyused in virology and show susceptibility for HPeV1 infection, which could be apositive control for infection. In addition, T84 cells (intestinal carcinoma),A549 cells (lung carcinoma cells) and DBTRG-5MG (glioblastoma cells) weresusceptible to HPeV infection; BHK21 cells (baby hamster kindney epithelialcells), HeLa cells (human cervival cancer cells) and J774A.1 cells (mousemacrophages) were not infected with HPeV1 (Fig. 2). Because HPeV1 infection causes clinicalsymptoms including respiratory- and gastrointestinal-tract and CNS symptoms[25], the celltropism results might reflect in part an association of HPeV1 infection and itstarget organs. Our data also support the cell tropism of HPeV1 Harris strain andother clinically isolated HPeV1 strains [31,37].

Bottom Line: Human parechoviruses (HPeVs), members of the family Picornaviridae, are associated with severe human clinical conditions such as gastrointestinal disease, encephalitis, meningitis, respiratory disease and neonatal sepsis.A facilitated cytopathic effect and increased viral titers were reached after serial viral passages in Vero cells, with viral genome mutation found in later passages.The characterization of the complete genome and infection features of HPeV1 provide comprehensive information about this newly isolated HPeV1 for further diagnosis, prevention or treatment strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan; Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; Department of Pediatrics; Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.

ABSTRACT
Human parechoviruses (HPeVs), members of the family Picornaviridae, are associated with severe human clinical conditions such as gastrointestinal disease, encephalitis, meningitis, respiratory disease and neonatal sepsis. A new contemporary strain of HPeV1, KVP6 (accession no. KC769584), was isolated from a clinical specimen. Full-genome alignment revealed that HPeV1 KVP6 shares high genome homology with the German strain of HPeV1, 7555312 (accession no. FM178558) and could be classified in the clade 1B group. An intertypic recombination was shown within the P2-P3 genome regions of HPeV1. Cell-type tropism test showed that T84 cells (colon carcinoma cells), A549 cells (lung carcinoma cells) and DBTRG-5MG cells (glioblastoma cells) were susceptible to HPeV1 infection, which might be relevant clinically. A facilitated cytopathic effect and increased viral titers were reached after serial viral passages in Vero cells, with viral genome mutation found in later passages. HPeV1 is sensitive to elevated temperature because 39C incubation impaired virion production. HPeV1 induced innate immunity with phosphorylation of interferon (IFN) regulatory transcription factor 3 and production of type I IFN in A549 but not T84 cells. Furthermore, type I IFN inhibited HPeV1 production in A549 cells but not T84 cells; T84 cells may be less responsive to type I IFN stimulation. Moreover, HPeV1-infected cells showed downregulated type I IFN activation, which indicated a type I IFN evasion mechanism. The characterization of the complete genome and infection features of HPeV1 provide comprehensive information about this newly isolated HPeV1 for further diagnosis, prevention or treatment strategies.

Show MeSH
Related in: MedlinePlus