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Fibrin gel as an injectable biodegradable scaffold and cell carrier for tissue engineering.

Li Y, Meng H, Liu Y, Lee BP - ScientificWorldJournal (2015)

Bottom Line: Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network.The ability for fibrin to cure in situ has been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues.Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.

ABSTRACT
Due to the increasing needs for organ transplantation and a universal shortage of donated tissues, tissue engineering emerges as a useful approach to engineer functional tissues. Although different synthetic materials have been used to fabricate tissue engineering scaffolds, they have many limitations such as the biocompatibility concerns, the inability to support cell attachment, and undesirable degradation rate. Fibrin gel, a biopolymeric material, provides numerous advantages over synthetic materials in functioning as a tissue engineering scaffold and a cell carrier. Fibrin gel exhibits excellent biocompatibility, promotes cell attachment, and can degrade in a controllable manner. Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network. The ability for fibrin to cure in situ has been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues. Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration. Here, we review the recent advancement in developing fibrin-based biomaterials for the development of injectable tissue engineering scaffold and cell carriers.

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Schematic illustration of two approaches to engineer desired tissue. Cells are isolated from biopsy and mixed with scaffold materials. Subsequently the mixture system is injected into patients' body (left). Alternatively, isolated cells are cultured on a scaffold in vitro and implanted into desired place after the formation of new functional tissue (right). Reprinted (adapted) with permission from [42]. Copyright (2001) American Chemical Society.
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fig2: Schematic illustration of two approaches to engineer desired tissue. Cells are isolated from biopsy and mixed with scaffold materials. Subsequently the mixture system is injected into patients' body (left). Alternatively, isolated cells are cultured on a scaffold in vitro and implanted into desired place after the formation of new functional tissue (right). Reprinted (adapted) with permission from [42]. Copyright (2001) American Chemical Society.

Mentions: Tissue engineering is a revolutionary strategy to solve the problem of shortage of donated organ or tissue. Cells are isolated from patient's tissue biopsy and seeded into a scaffold, which provides mechanical support for cell migration, proliferation, and tissue regeneration. There are two approaches to engineer tissues (Figure 2). One of them is to inject the mixture of scaffold precursor and cells into patients' body [42]. The other approach involves culturing the scaffold in vitro and implanting the subsequent engineered tissue into patients' body. Occasionally, it is necessary to encapsulate cells in a delivery carrier in order to improve the viability of transplanted cells and tissue regeneration. Therefore, cells will be mixed with delivery carrier first and then the mixture system will be delivered into a scaffold.


Fibrin gel as an injectable biodegradable scaffold and cell carrier for tissue engineering.

Li Y, Meng H, Liu Y, Lee BP - ScientificWorldJournal (2015)

Schematic illustration of two approaches to engineer desired tissue. Cells are isolated from biopsy and mixed with scaffold materials. Subsequently the mixture system is injected into patients' body (left). Alternatively, isolated cells are cultured on a scaffold in vitro and implanted into desired place after the formation of new functional tissue (right). Reprinted (adapted) with permission from [42]. Copyright (2001) American Chemical Society.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4380102&req=5

fig2: Schematic illustration of two approaches to engineer desired tissue. Cells are isolated from biopsy and mixed with scaffold materials. Subsequently the mixture system is injected into patients' body (left). Alternatively, isolated cells are cultured on a scaffold in vitro and implanted into desired place after the formation of new functional tissue (right). Reprinted (adapted) with permission from [42]. Copyright (2001) American Chemical Society.
Mentions: Tissue engineering is a revolutionary strategy to solve the problem of shortage of donated organ or tissue. Cells are isolated from patient's tissue biopsy and seeded into a scaffold, which provides mechanical support for cell migration, proliferation, and tissue regeneration. There are two approaches to engineer tissues (Figure 2). One of them is to inject the mixture of scaffold precursor and cells into patients' body [42]. The other approach involves culturing the scaffold in vitro and implanting the subsequent engineered tissue into patients' body. Occasionally, it is necessary to encapsulate cells in a delivery carrier in order to improve the viability of transplanted cells and tissue regeneration. Therefore, cells will be mixed with delivery carrier first and then the mixture system will be delivered into a scaffold.

Bottom Line: Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network.The ability for fibrin to cure in situ has been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues.Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.

ABSTRACT
Due to the increasing needs for organ transplantation and a universal shortage of donated tissues, tissue engineering emerges as a useful approach to engineer functional tissues. Although different synthetic materials have been used to fabricate tissue engineering scaffolds, they have many limitations such as the biocompatibility concerns, the inability to support cell attachment, and undesirable degradation rate. Fibrin gel, a biopolymeric material, provides numerous advantages over synthetic materials in functioning as a tissue engineering scaffold and a cell carrier. Fibrin gel exhibits excellent biocompatibility, promotes cell attachment, and can degrade in a controllable manner. Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network. The ability for fibrin to cure in situ has been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues. Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration. Here, we review the recent advancement in developing fibrin-based biomaterials for the development of injectable tissue engineering scaffold and cell carriers.

Show MeSH