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Vitis vinifera (Muscat Variety) Seed Ethanolic Extract Preserves Activity Levels of Enzymes and Histology of the Liver in Adult Male Rats with Diabetes.

Giribabu N, Eswar Kumar K, Swapna Rekha S, Muniandy S, Salleh N - Evid Based Complement Alternat Med (2015)

Bottom Line: Additionally, the levels of ALT, AST, and TBARS in liver homogenates were decreased.Histopathological changes in the liver were reduced.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
The effect of V. vinifera seeds on carbohydrate metabolizing enzymes and other enzymes of the liver in diabetes is currently unknown. We therefore investigated changes in the activity levels of these enzymes following V. vinifera seed extract administration to diabetic rats. Methods. V. vinifera seed ethanolic extract (250 and 500 mg/kg/day) or glibenclamide (600 μg/kg/day) was administered to streptozotocin-induced male diabetic rats for 28 consecutive days. At the end of treatment, liver was harvested and activity levels of various liver enzymes were determined. Levels of thiobarbituric acid reactive substances (TBARS) were measured in liver homogenates and liver histopathological changes were observed. Results. V. vinifera seed ethanolic extract was able to prevent the decrease in ICDH, SDH, MDH, and G-6-PDH and the increase in LDH activity levels in liver homogenates. The seed extract also caused serum levels of ALT, AST, ALP, ACP, GGT, and total bilirubin to decrease while causing total proteins to increase. Additionally, the levels of ALT, AST, and TBARS in liver homogenates were decreased. Histopathological changes in the liver were reduced. Conclusion. Near normal activity levels of various enzymes and histology of the liver following V. vinifera seed ethanolic extract administration may be due to decrease in liver oxidative stress in diabetes.

No MeSH data available.


Related in: MedlinePlus

Effect of the seed ethanolic extract of V. vinifera on liver histology. Representative images of the liver in (a) normal, (b) STZ-induced diabetic rats, (c) diabetic rats treated with 250 mg/kg/day V. vinifera seed extract, (d) diabetic rats treated with 500 mg/kg/day V. vinifera seed extract, and (e) diabetic rats treated with 600 μg/kg/day glibenclamide. Images were taken under 20× magnification. Scale bar represents 50 μm. In diabetic rats, several areas of moderate to severe necrosis could be seen around the central vein. Mild to absence of necrotic changes could be seen following treatment with 500 mg/kg/day V. vinifera or glibenclamide to diabetic rats. H = hepatocytes, CV = central vein, S = sinusoid, and N = necrosis (40× magnification).
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fig1: Effect of the seed ethanolic extract of V. vinifera on liver histology. Representative images of the liver in (a) normal, (b) STZ-induced diabetic rats, (c) diabetic rats treated with 250 mg/kg/day V. vinifera seed extract, (d) diabetic rats treated with 500 mg/kg/day V. vinifera seed extract, and (e) diabetic rats treated with 600 μg/kg/day glibenclamide. Images were taken under 20× magnification. Scale bar represents 50 μm. In diabetic rats, several areas of moderate to severe necrosis could be seen around the central vein. Mild to absence of necrotic changes could be seen following treatment with 500 mg/kg/day V. vinifera or glibenclamide to diabetic rats. H = hepatocytes, CV = central vein, S = sinusoid, and N = necrosis (40× magnification).

Mentions: Figure 1 shows histopathological changes while Table 3 shows semiquantitative analyses of inflammatory changes in the liver of diabetic rats receiving V. vinifera seed extract or glibenclamide treatment. In normal, nondiabetic rats, a distinct and well-arranged hepatocytes, sinusoids, and central vein could be seen (Figure 1(a)). Meanwhile, in diabetic rats, hepatocytes were disorganized with several areas of necrosis. Sinusoids were enlarged with the wall of veins thickened (Figure 1(b)). V. vinifera seed extract or glibenclamide treatments prevented these changes as evidenced by lesser signs of necrosis, lack of central hemorrhagic necrosis, mild sinusoid hyperemia, and mild connective tissue inflammation in the portal region (Figures 1(c), 1(d), and 1(e)).


Vitis vinifera (Muscat Variety) Seed Ethanolic Extract Preserves Activity Levels of Enzymes and Histology of the Liver in Adult Male Rats with Diabetes.

Giribabu N, Eswar Kumar K, Swapna Rekha S, Muniandy S, Salleh N - Evid Based Complement Alternat Med (2015)

Effect of the seed ethanolic extract of V. vinifera on liver histology. Representative images of the liver in (a) normal, (b) STZ-induced diabetic rats, (c) diabetic rats treated with 250 mg/kg/day V. vinifera seed extract, (d) diabetic rats treated with 500 mg/kg/day V. vinifera seed extract, and (e) diabetic rats treated with 600 μg/kg/day glibenclamide. Images were taken under 20× magnification. Scale bar represents 50 μm. In diabetic rats, several areas of moderate to severe necrosis could be seen around the central vein. Mild to absence of necrotic changes could be seen following treatment with 500 mg/kg/day V. vinifera or glibenclamide to diabetic rats. H = hepatocytes, CV = central vein, S = sinusoid, and N = necrosis (40× magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig1: Effect of the seed ethanolic extract of V. vinifera on liver histology. Representative images of the liver in (a) normal, (b) STZ-induced diabetic rats, (c) diabetic rats treated with 250 mg/kg/day V. vinifera seed extract, (d) diabetic rats treated with 500 mg/kg/day V. vinifera seed extract, and (e) diabetic rats treated with 600 μg/kg/day glibenclamide. Images were taken under 20× magnification. Scale bar represents 50 μm. In diabetic rats, several areas of moderate to severe necrosis could be seen around the central vein. Mild to absence of necrotic changes could be seen following treatment with 500 mg/kg/day V. vinifera or glibenclamide to diabetic rats. H = hepatocytes, CV = central vein, S = sinusoid, and N = necrosis (40× magnification).
Mentions: Figure 1 shows histopathological changes while Table 3 shows semiquantitative analyses of inflammatory changes in the liver of diabetic rats receiving V. vinifera seed extract or glibenclamide treatment. In normal, nondiabetic rats, a distinct and well-arranged hepatocytes, sinusoids, and central vein could be seen (Figure 1(a)). Meanwhile, in diabetic rats, hepatocytes were disorganized with several areas of necrosis. Sinusoids were enlarged with the wall of veins thickened (Figure 1(b)). V. vinifera seed extract or glibenclamide treatments prevented these changes as evidenced by lesser signs of necrosis, lack of central hemorrhagic necrosis, mild sinusoid hyperemia, and mild connective tissue inflammation in the portal region (Figures 1(c), 1(d), and 1(e)).

Bottom Line: Additionally, the levels of ALT, AST, and TBARS in liver homogenates were decreased.Histopathological changes in the liver were reduced.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

ABSTRACT
The effect of V. vinifera seeds on carbohydrate metabolizing enzymes and other enzymes of the liver in diabetes is currently unknown. We therefore investigated changes in the activity levels of these enzymes following V. vinifera seed extract administration to diabetic rats. Methods. V. vinifera seed ethanolic extract (250 and 500 mg/kg/day) or glibenclamide (600 μg/kg/day) was administered to streptozotocin-induced male diabetic rats for 28 consecutive days. At the end of treatment, liver was harvested and activity levels of various liver enzymes were determined. Levels of thiobarbituric acid reactive substances (TBARS) were measured in liver homogenates and liver histopathological changes were observed. Results. V. vinifera seed ethanolic extract was able to prevent the decrease in ICDH, SDH, MDH, and G-6-PDH and the increase in LDH activity levels in liver homogenates. The seed extract also caused serum levels of ALT, AST, ALP, ACP, GGT, and total bilirubin to decrease while causing total proteins to increase. Additionally, the levels of ALT, AST, and TBARS in liver homogenates were decreased. Histopathological changes in the liver were reduced. Conclusion. Near normal activity levels of various enzymes and histology of the liver following V. vinifera seed ethanolic extract administration may be due to decrease in liver oxidative stress in diabetes.

No MeSH data available.


Related in: MedlinePlus