Radioprotector WR-2721 and mitigating peptidoglycan synergistically promote mouse survival through the amelioration of intestinal and bone marrow damage.
Bottom Line: Furthermore, combined PGN and WR-2721 treatment had an obviously synergistic radio-protective effect in nucleated cells in the bone marrow, including GEMM progenitors and CD45(+)CD34(+) cells 4 days after 10 Gy IR.The NF-κB p65 subunit was translocated to the nucleus, and phosphate-IκBα (Ser32/Ser36) was detected after stimulation with either PGN or WR-2721, which indicates that these two agents act synergistically through the activation of the NF-κB pathway.Administration of PGN in combination with WR-2721 was demonstrated to have a synergistic effect on the increase in haematopoietic cells and intestinal reconstitution, as well as improved survival in lethally irradiated mice, but resulted in some degree of an immune disorder.
Affiliation: School of Radiation Medicine and Protection, Soochow University, No. 199 Ren'ai Road, Suzhou 215123, PR China.Show MeSH
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Mentions: As xylose is not metabolised in the body, the d-xylose absorption test is used to measure the level of d-xylose in blood or urine samples to help diagnose problems that prevent the small intestine from absorbing nutrients in food. In this study, urine xylose levels were tested 2 h after a test feeding dose at 3, 7, 15 and 35 days after 10 Gy IR. Normal unirradiated mice demonstrated 100% xylose absorption. As expected, a progressive reduction in xylose absorption rates was observed in irradiated mice. However, intestinal absorptive function was elevated in the combined treatment group compared with the IR group (Fig. 4), indicating that the combination of WR-2721 and PGN best restituted the intestinal villi damaged by IR compared with WR-2721 or PGN treatment alone.Fig. 4.
Affiliation: School of Radiation Medicine and Protection, Soochow University, No. 199 Ren'ai Road, Suzhou 215123, PR China.