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In vivo measurement of dose distribution in patients' lymphocytes: helical tomotherapy versus step-and-shoot IMRT in prostate cancer.

Zwicker F, Swartman B, Roeder F, Sterzing F, Hauswald H, Thieke C, Weber KJ, Huber PE, Schubert K, Debus J, Herfarth K - J. Radiat. Res. (2014)

Bottom Line: The isolated leukocytes were fixed 2 h after radiation.The dose exposure range, between 45 and 100%, was equal with both radiation techniques.Differences in the constructional buildup of the different linear accelerator systems, e.g. the flattening filter, may be the cause thereof.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany Clinical Cooperation Unit Radiation Oncology, German Cancer Research Center (DKFZ), Heidelberg, Germany f.zwicker@dkfz.de.

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The average of mean number of gamma-H2AX foci per nucleus in irradiated lymphocytes and negative controls of 20 patients per group is shown (SSIMRT and TOMO). Standard errors are shown. All patients were irradiated in the prostate region, and venous blood was taken before (control) and 10 min after their first irradiation fraction. Lymphocytes were fixed 2 h after the end of the irradiation. In the negative control, 50 lymphocytes were analyzed per patient; in the irradiated samples, 300 lymphocytes were analyzed per patient.
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RRU096F2: The average of mean number of gamma-H2AX foci per nucleus in irradiated lymphocytes and negative controls of 20 patients per group is shown (SSIMRT and TOMO). Standard errors are shown. All patients were irradiated in the prostate region, and venous blood was taken before (control) and 10 min after their first irradiation fraction. Lymphocytes were fixed 2 h after the end of the irradiation. In the negative control, 50 lymphocytes were analyzed per patient; in the irradiated samples, 300 lymphocytes were analyzed per patient.

Mentions: For the investigated lymphocytes of 20 patients per group, the mean numbers of gamma-H2AX foci per nucleus were 0.47 (SSIMRT) and 0.24 (TOMO) (P < 0.05) in the irradiated samples (Fig. 2), whereas the non-irradiated controls scored 0.037 gamma-H2AX foci per nucleus on average (P < 0.05).Fig. 2.


In vivo measurement of dose distribution in patients' lymphocytes: helical tomotherapy versus step-and-shoot IMRT in prostate cancer.

Zwicker F, Swartman B, Roeder F, Sterzing F, Hauswald H, Thieke C, Weber KJ, Huber PE, Schubert K, Debus J, Herfarth K - J. Radiat. Res. (2014)

The average of mean number of gamma-H2AX foci per nucleus in irradiated lymphocytes and negative controls of 20 patients per group is shown (SSIMRT and TOMO). Standard errors are shown. All patients were irradiated in the prostate region, and venous blood was taken before (control) and 10 min after their first irradiation fraction. Lymphocytes were fixed 2 h after the end of the irradiation. In the negative control, 50 lymphocytes were analyzed per patient; in the irradiated samples, 300 lymphocytes were analyzed per patient.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4380044&req=5

RRU096F2: The average of mean number of gamma-H2AX foci per nucleus in irradiated lymphocytes and negative controls of 20 patients per group is shown (SSIMRT and TOMO). Standard errors are shown. All patients were irradiated in the prostate region, and venous blood was taken before (control) and 10 min after their first irradiation fraction. Lymphocytes were fixed 2 h after the end of the irradiation. In the negative control, 50 lymphocytes were analyzed per patient; in the irradiated samples, 300 lymphocytes were analyzed per patient.
Mentions: For the investigated lymphocytes of 20 patients per group, the mean numbers of gamma-H2AX foci per nucleus were 0.47 (SSIMRT) and 0.24 (TOMO) (P < 0.05) in the irradiated samples (Fig. 2), whereas the non-irradiated controls scored 0.037 gamma-H2AX foci per nucleus on average (P < 0.05).Fig. 2.

Bottom Line: The isolated leukocytes were fixed 2 h after radiation.The dose exposure range, between 45 and 100%, was equal with both radiation techniques.Differences in the constructional buildup of the different linear accelerator systems, e.g. the flattening filter, may be the cause thereof.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany Clinical Cooperation Unit Radiation Oncology, German Cancer Research Center (DKFZ), Heidelberg, Germany f.zwicker@dkfz.de.

Show MeSH
Related in: MedlinePlus