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Facilitation of synaptic transmission in the anterior cingulate cortex in viscerally hypersensitive rats.

Wang J, Zhang X, Cao B, Liu J, Li Y - Cereb. Cortex (2013)

Bottom Line: However, only a few reports have indicated the synaptic plasticity of ACC in vivo.In conclusion, we demonstrated for the first time that visceral hypersensitivity is associated with alterations of synaptic plasticity in the ACC.The ACC synaptic strengthening in chronic visceral pain may engage signal transduction pathways that are in common with those activated by electrical stimulation, and serves as an attractive cellular model of functional visceral pain.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Laboratory, Department of Biology and Chemistry, Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Hong Kong, China Key Laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen 518057, PR China.

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Effects of GABAA receptor antagonist on LTP-like synaptic plasticity in control and VH rats. (A) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in control rats. (B) There was no obvious change in LTP-like plasticity in the MT-ACC synapse in control rats. (C) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in VH rats. (D) LTP-like plasticity in the MT-ACC synapse was facilitated in VH rats after BIC application. Results are expressed as mean ± SEM.
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BHT273F5: Effects of GABAA receptor antagonist on LTP-like synaptic plasticity in control and VH rats. (A) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in control rats. (B) There was no obvious change in LTP-like plasticity in the MT-ACC synapse in control rats. (C) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in VH rats. (D) LTP-like plasticity in the MT-ACC synapse was facilitated in VH rats after BIC application. Results are expressed as mean ± SEM.

Mentions: In separate groups of control (n = 8) and VH rats (n = 10), GABAA receptor antagonist BIC (100 µM) was applied to the ACC through reverse microdialysis before induction of LTP-like plasticity by TBS to the MT. Representative recording curves of LFP in response to MT stimulation (400 µA, evoked 15% of maximum amplitude of the LFP) pre- and post-TBS in control and VH rats treated with vehicle (ACSF) or BIC are shown in Figure 5A,C. In our experimental conditions, there were no significant changes in the LTP-like synaptic plasticity in control rats after BIC application (n = 4) compared with those treated with vehicle (n = 4) (Fig 5B). However, we found that, in VH rats, LTP-like synaptic plasticity was significantly facilitated only if GABAAergic tone was reduced by local application of GABAA receptor antagonist (n = 5), but not vehicle (n = 5). The LFP amplitude following TBS conditioning reached 125.9 ± 2.7% of pre-TBS values and lasted for at least 40 min in VH rats (Fig. 5D).Figure 5.


Facilitation of synaptic transmission in the anterior cingulate cortex in viscerally hypersensitive rats.

Wang J, Zhang X, Cao B, Liu J, Li Y - Cereb. Cortex (2013)

Effects of GABAA receptor antagonist on LTP-like synaptic plasticity in control and VH rats. (A) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in control rats. (B) There was no obvious change in LTP-like plasticity in the MT-ACC synapse in control rats. (C) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in VH rats. (D) LTP-like plasticity in the MT-ACC synapse was facilitated in VH rats after BIC application. Results are expressed as mean ± SEM.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379994&req=5

BHT273F5: Effects of GABAA receptor antagonist on LTP-like synaptic plasticity in control and VH rats. (A) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in control rats. (B) There was no obvious change in LTP-like plasticity in the MT-ACC synapse in control rats. (C) Representative curves of LFP responses in the ACC to MT stimuli pre- and post-TBS after vehicle or BIC application in VH rats. (D) LTP-like plasticity in the MT-ACC synapse was facilitated in VH rats after BIC application. Results are expressed as mean ± SEM.
Mentions: In separate groups of control (n = 8) and VH rats (n = 10), GABAA receptor antagonist BIC (100 µM) was applied to the ACC through reverse microdialysis before induction of LTP-like plasticity by TBS to the MT. Representative recording curves of LFP in response to MT stimulation (400 µA, evoked 15% of maximum amplitude of the LFP) pre- and post-TBS in control and VH rats treated with vehicle (ACSF) or BIC are shown in Figure 5A,C. In our experimental conditions, there were no significant changes in the LTP-like synaptic plasticity in control rats after BIC application (n = 4) compared with those treated with vehicle (n = 4) (Fig 5B). However, we found that, in VH rats, LTP-like synaptic plasticity was significantly facilitated only if GABAAergic tone was reduced by local application of GABAA receptor antagonist (n = 5), but not vehicle (n = 5). The LFP amplitude following TBS conditioning reached 125.9 ± 2.7% of pre-TBS values and lasted for at least 40 min in VH rats (Fig. 5D).Figure 5.

Bottom Line: However, only a few reports have indicated the synaptic plasticity of ACC in vivo.In conclusion, we demonstrated for the first time that visceral hypersensitivity is associated with alterations of synaptic plasticity in the ACC.The ACC synaptic strengthening in chronic visceral pain may engage signal transduction pathways that are in common with those activated by electrical stimulation, and serves as an attractive cellular model of functional visceral pain.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Laboratory, Department of Biology and Chemistry, Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Hong Kong, China Key Laboratory of Biochip Technology, Shenzhen Biotech and Health Centre, City University of Hong Kong, Shenzhen 518057, PR China.

Show MeSH
Related in: MedlinePlus