A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.
Bottom Line: Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs.Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized.These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
Affiliation: Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan email@example.com.Show MeSH
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Mentions: Based on the concatenated nucleotide sequences of seven housekeeping genes used for MLST, we determined the evolutionary relationships of all E. coli O-serogroup reference strains (Fig. 3). This analysis revealed that the members of five groups sharing the common O-AGCs (Gp8, Gp10, Gp11, Gp14, and Gp15) and two members (O17 and O77) of Gp9 were found in closely related lineages. However, the members of other groups (and three members of Gp9) were found in distinct evolutionary lineages. For example, O20 and O137, both carrying the Gp1 O-AGC, were found in two distinct lineages, each belonging to phylogroups A and E/D, respectively, and five serogroups (O17/O77, O44, O73, and O106) belonging to Gp9 were found in multiple lineages (A, E/D, and B1).Figure 3.
Affiliation: Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan firstname.lastname@example.org.