Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish.
Bottom Line: The sex-specific linkage maps spanned a total of 4,495.1 cM in females and 2,593.7 cM in males, presenting a ratio of 1.7 : 1 between female and male in recombination fraction.After integration with the previously established physical map, over 87% of physical map contigs were anchored to the linkage groups that covered a physical length of 867 Mb, accounting for ∼90% of the catfish genome.The integrated map provides a valuable tool for validating and improving the catfish whole-genome assembly and facilitates fine-scale QTL mapping and positional cloning of genes responsible for economically important traits.
Affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA.Show MeSH
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Mentions: The integration of linkage map and physical map enabled the cross-validation of the quality of the physical map and the genetic map (Table 6). A total of 1,467 physical map contigs containing 2,961 BES-associated SNPs were placed onto linkage maps. With 615 physical map contigs, two or more SNP markers were mapped that allowed the determination of orientation of the physical map contigs along the chromosome. With such contigs, the quality of physical map contigs was assessed based on the mapped markers. An illustration with linkage group 12 is provided in Fig. 6. The vast majority of physical contigs were validated, but a small fraction of physical map contigs was found incorrectly assembled, or the ordering of the SNP markers onto the map was in error.Table 6.
Affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA.