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Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish.

Li Y, Liu S, Qin Z, Waldbieser G, Wang R, Sun L, Bao L, Danzmann RG, Dunham R, Liu Z - DNA Res. (2014)

Bottom Line: In the present work, we constructed a high-density and high-resolution genetic map for channel catfish with three large resource families genotyped using the catfish 250K single-nucleotide polymorphism (SNP) array.After integration with the previously established physical map, over 87% of physical map contigs were anchored to the linkage groups that covered a physical length of 867 Mb, accounting for ∼90% of the catfish genome.The integrated map provides a valuable tool for validating and improving the catfish whole-genome assembly and facilitates fine-scale QTL mapping and positional cloning of genes responsible for economically important traits.

View Article: PubMed Central - PubMed

Affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA.

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Related in: MedlinePlus

Illustration of sex-averaged linkage map.
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DSU038F3: Illustration of sex-averaged linkage map.

Mentions: The sex-averaged map was constructed using markers that were heterozygous in both parents. As summarized in Table 4, a total of 29,081 SNPs were mapped, which consisted of 15,598 unique markers. The sex-averaged map spanned 3,505.4 cM with an average marker interval of 0.22 cM, ranging from 0.17 cM/marker (LG12) to 0.30 cM/marker (LG18). The genetic map is illustrated in Fig. 3. The detailed map information was provided in Supplementary data S1.Table 4.


Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish.

Li Y, Liu S, Qin Z, Waldbieser G, Wang R, Sun L, Bao L, Danzmann RG, Dunham R, Liu Z - DNA Res. (2014)

Illustration of sex-averaged linkage map.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4379976&req=5

DSU038F3: Illustration of sex-averaged linkage map.
Mentions: The sex-averaged map was constructed using markers that were heterozygous in both parents. As summarized in Table 4, a total of 29,081 SNPs were mapped, which consisted of 15,598 unique markers. The sex-averaged map spanned 3,505.4 cM with an average marker interval of 0.22 cM, ranging from 0.17 cM/marker (LG12) to 0.30 cM/marker (LG18). The genetic map is illustrated in Fig. 3. The detailed map information was provided in Supplementary data S1.Table 4.

Bottom Line: In the present work, we constructed a high-density and high-resolution genetic map for channel catfish with three large resource families genotyped using the catfish 250K single-nucleotide polymorphism (SNP) array.After integration with the previously established physical map, over 87% of physical map contigs were anchored to the linkage groups that covered a physical length of 867 Mb, accounting for ∼90% of the catfish genome.The integrated map provides a valuable tool for validating and improving the catfish whole-genome assembly and facilitates fine-scale QTL mapping and positional cloning of genes responsible for economically important traits.

View Article: PubMed Central - PubMed

Affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA.

Show MeSH
Related in: MedlinePlus