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Low mir-372 expression correlates with poor prognosis and tumor metastasis in hepatocellular carcinoma.

Wu G, Wang Y, Lu X, He H, Liu H, Meng X, Xia S, Zheng K, Liu B - BMC Cancer (2015)

Bottom Line: However, results have been conflicting regarding its expression levels and role in HCC.Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression. miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis.Upregulated expression by mir-372 mimic transfection inhibited proliferation and invasion capacity in HCC cells. miR-372 may play an important role in hepatic carcinogenesis and may serve as a new target or method to detect and treat HCC in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. wgzwl@hotmail.com.

ABSTRACT

Background: Recent studies have shown that miR-372 plays important roles in hepatocellular carcinoma (HCC) progression. However, results have been conflicting regarding its expression levels and role in HCC.

Methods: RT-PCR and in situ hybridization was used to evaluate miR-372 expression in HCC tissues and cell lines. The methylation status of neighboring CpG islands upstream of the miR-372 promoter was analyzed by methylation-specific PCR (MSP). Transfection of miR-372 mimic into HCC cell lines was used to evaluate cellular proliferation and invasion. Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression.

Results: miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis. Hypermethylation of miR-372 was detected in HCC cell lines and tissues, and miR-372 expression was restored upon 5-aza-dCyd treatment. Upregulated expression by mir-372 mimic transfection inhibited proliferation and invasion capacity in HCC cells.

Conclusions: miR-372 may play an important role in hepatic carcinogenesis and may serve as a new target or method to detect and treat HCC in the future.

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Related in: MedlinePlus

Overall survival of HCC patients in relation to mir-372 expression levels according to in situ hybridization. Survival of HCC patients with high mir-372 expression versus low expression.
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Fig6: Overall survival of HCC patients in relation to mir-372 expression levels according to in situ hybridization. Survival of HCC patients with high mir-372 expression versus low expression.

Mentions: We next analyzed the association between the miR-372 expression and various clinicopathological factors of the HCC patients. Interestingly, the expression levels of miR-372 were significantly lower in HCC tissues with advanced TNM stage compared with those with early TNM stage (P = 0.003) and miR-372 low expression tissues had more tumor metastasis (P < 0.001, Table 1). Patients with lower miR-372 expression had poorer prognosis. The overall survival was significantly lower in patients with low miR-372 expression than in patients with high miR-372 expression (P = 0.004) (Figure 6). In addition, multivariate analysis demonstrated that miR-372 levels, metastasis and AFP status were significant prognostic factors for HCC patients (Table 2).Table 1


Low mir-372 expression correlates with poor prognosis and tumor metastasis in hepatocellular carcinoma.

Wu G, Wang Y, Lu X, He H, Liu H, Meng X, Xia S, Zheng K, Liu B - BMC Cancer (2015)

Overall survival of HCC patients in relation to mir-372 expression levels according to in situ hybridization. Survival of HCC patients with high mir-372 expression versus low expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4379970&req=5

Fig6: Overall survival of HCC patients in relation to mir-372 expression levels according to in situ hybridization. Survival of HCC patients with high mir-372 expression versus low expression.
Mentions: We next analyzed the association between the miR-372 expression and various clinicopathological factors of the HCC patients. Interestingly, the expression levels of miR-372 were significantly lower in HCC tissues with advanced TNM stage compared with those with early TNM stage (P = 0.003) and miR-372 low expression tissues had more tumor metastasis (P < 0.001, Table 1). Patients with lower miR-372 expression had poorer prognosis. The overall survival was significantly lower in patients with low miR-372 expression than in patients with high miR-372 expression (P = 0.004) (Figure 6). In addition, multivariate analysis demonstrated that miR-372 levels, metastasis and AFP status were significant prognostic factors for HCC patients (Table 2).Table 1

Bottom Line: However, results have been conflicting regarding its expression levels and role in HCC.Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression. miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis.Upregulated expression by mir-372 mimic transfection inhibited proliferation and invasion capacity in HCC cells. miR-372 may play an important role in hepatic carcinogenesis and may serve as a new target or method to detect and treat HCC in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110001, China. wgzwl@hotmail.com.

ABSTRACT

Background: Recent studies have shown that miR-372 plays important roles in hepatocellular carcinoma (HCC) progression. However, results have been conflicting regarding its expression levels and role in HCC.

Methods: RT-PCR and in situ hybridization was used to evaluate miR-372 expression in HCC tissues and cell lines. The methylation status of neighboring CpG islands upstream of the miR-372 promoter was analyzed by methylation-specific PCR (MSP). Transfection of miR-372 mimic into HCC cell lines was used to evaluate cellular proliferation and invasion. Prognostic significance was analyzed by the Kaplan-Meier survival method and Cox regression.

Results: miR-372 was expressed at lower levels in HCC tissues compared with controls and was related to tumor metastasis and poor prognosis. Hypermethylation of miR-372 was detected in HCC cell lines and tissues, and miR-372 expression was restored upon 5-aza-dCyd treatment. Upregulated expression by mir-372 mimic transfection inhibited proliferation and invasion capacity in HCC cells.

Conclusions: miR-372 may play an important role in hepatic carcinogenesis and may serve as a new target or method to detect and treat HCC in the future.

Show MeSH
Related in: MedlinePlus